Low Molecular Weight Heparin Nebulization Attenuates Acute Lung Injury

Abstract

Background As acute lung injury (ALI) caused high mortality rate, it is important to explore the protection and treatment of ALI. The aim of the current study is to evaluate the effect of low molecular weight heparin (LMWH) nebulization on attenuating acute lung injury and the associated mechanism. Methods The arterial blood gas, total protein content in bronchoalveolar lavage fluid, lung wet/dry weight ratio, malondialdehyde (MDA) content, superoxide dismutase (SOD) and glutathione peroxidase (GSH-Px) activity, and Akt phosphorylation were evaluated after the ALI rabbits were treated with or without LMWH nebulization. Results PaO2 was increased and lung wet/dry weight ratio as well as total protein content in BALF was decreased after LMWH nebulization. After the application of LMWH nebulization therapy, the SOD and GSH-Px activity was rebounded and the increase of MDA content was significantly inhibited. The Akt protein phosphorylation level was decreased after LMWH nebulization therapy. Conclusions LMWH nebulization treatment can relieve the traumatic ALI in rabbits and inhibit oxidative stress possibly by suppressing the Akt phosphorylation.

Therapeutic versus prophylactic anticoagulation for severe COVID-19: A randomized phase II clinical trial (HESACOVID)

“…Despite evidence about coagulation activation and impaired fibrinolysis leading to fibrin deposition in the alveolar compartment in non-COVID-19 ARDS patients [18], data about therapeutic anticoagulation's efficacy and safety is very limited in these patients [19]. Some recent investigations showed that low molecular weight heparin (LMWH) could reduce acute lung injury (ALI) in experimental models [20,21] [15]. However, these individual trials' methodological quality was relatively low, and they were available only in Chinese databases.…”

Anti-diabetic activity of field cricket glycosaminoglycan by ameliorating oxidative stress

“…7. The postulated anti-oxidative action of cricket glycosaminoglycan in type 2 diabetic mice is supported by findings suggesting that GAG such as the low-molecular-weight heparin, increases SOD levels and inhibits oxidative stress [31] and that glycosaminoglycan derived from Urechis unicinctus significantly enhances liver SOD and GSH-Px activities [24]. Further clinical studies are needed to verify the proposed mechanism.…”

Low molecular weight heparin treatment reduced apoptosis and oxidative cytotoxicity in the thrombocytes of patients with recurrent pregnancy loss and thrombophilia: Involvements of TRPM2 and TRPV1 channels

“…Because the oxidation of cysteine groups is required for the activation of TRPM2 and TRPV1, recent studies suggested that the decrease of GSH and GSH‐Px in plasma and cells induces the activation of TRPM2 and TRPV1 26,51,52 . Through the elevation of GSH and GSH‐Px in the patients' blood, LMWH therapy has an antioxidant effect 29 . As a result, LPO and cytROS formation is reduced by antioxidant treatments or by boosting antioxidants in body cells 13,14,52 .…”

“…[26][27][28] Patients with reproductive abnormalities are treated with LMWH, and this medication has an antioxidant effect by increasing the levels of GSH and GSH-Px in the patients' blood. 29,30 The regulation of TRPM2 activation, TRPV1 stimulation, production of oxidative toxicity, and apoptotic indicators may result in the effects.…”

“…However, the negative apoptotic and oxidative effects in the cells of abortion patients were reduced by the therapy with antioxidants, which inhibited the TRPM2 and TRPV1 26–28 . Patients with reproductive abnormalities are treated with LMWH, and this medication has an antioxidant effect by increasing the levels of GSH and GSH‐Px in the patients' blood 29,30 . The regulation of TRPM2 activation, TRPV1 stimulation, production of oxidative toxicity, and apoptotic indicators may result in the effects.…”