Low load strength training, associated with or without blood flow restriction increased NO production and decreased production of reactive oxygen species in rats aorta

Garcia, Nádia Fagundes; Moraes, Camila de; Rebelo, M.S.; Castro, Fábio Morato; Peters, Savanah Maria Gertrudis Rosa; Pernomian, Laena; Mello, Marcela M. Blascke de; Castro, Michele M.; Puggina, Enrico Fuini

doi:10.1016/j.lfs.2022.120350

Cited by 2 publications

(3 citation statements)

References 33 publications

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“…The phenylephrine sensitivity was reduced by 5-fold in the ITR group. A similar result was observed in intact or endothelium-denuded superior mesenteric artery from male Wistar submitted to swimming training 37 On the other hand, continuous training increased the alfa-adrenergic sensitivity by 4-fold and this result was similar to those observed in aortic rings from male Wistar rats that underwent physical exercise for four weeks 34 . An increase in male Sprague Dawley femoral artery vasoconstriction was seen after high-intensity treadmill exercise due to an augmented sympathetic stimulation 39 .…”

Section: Discussionsupporting

confidence: 67%

“…The pioneer studies showing the effect of physical exercise on endothelial cells were conducted in the 90s. It has been proposed that the increase of pulsatile blood flow on endothelial cells, promotes a potential mechanical stimulus for NO production 33 as was seen after 4 weeks of exercise training 34 . In addition, the bioavailability of NO could be increased either by the rise of antioxidant enzyme expression or reduction of reactive oxygen species (ROS) 9,11 .…”

Section: Discussionmentioning

confidence: 99%

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Interval or continuous aerobic exercise performed 3 days a week increases endothelium-dependent relaxation in female rats fed with fructose

Joaquim

Oharomari

Vale³

et al. 2022

Motriz: rev. educ. fis.

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We investigated the effects of continuous or interval aerobic exercise training on vascular reactivity of female rats fed with fructose. Methods: Female Wistar rats (8-wk old) were divided into: sedentary (SD), continuous training (CTR), and interval training (ITR). Moderate intensity training protocols consisted of running 3 days/week for 7 weeks. CTR ran 40 min at 30%À 40% of the maximal speed (MS) and TRI consisted of 7 sets of 1 min at 70% of MS followed by 3 min at 35% of MS. Animals were fed with standard chow and fructose (10%) in drinking water. Concentration-response curves to acetylcholine and phenylephrine, and oxidative stress biomarkers, were determined in the aorta. Body weight gain, visceral fat, and plasma triglycerides and glucose were also evaluated. Results:Endothelium-dependent relaxation was significantly increased by both exercise regimens (CTR: Emax = 85 ± 6% and ITR: Emax = 84 ± 1%) compared to sedentary rats (SD: Emax = 62 ± 5%). The contractile maximal response was not different but phenylephrine potency was increased in CTR (pEC 50 : 8.41 ± 0.19) and reduced in ITR (pEC 50 : 7.06 ± 0.11) compared to SD (pEC 50 : 7.77 ± 0.08). In addition, the generation of superoxide was lower in trained groups as compared with sedentary (about −28% in CTR and −22% in ITR). TBARS and nitrate/nitrite levels were not modified. Compared to the SD group, ITR gained 39% less body weight and CTR has 29% less visceral fat. Glucose and triglycerides were not modified. Conclusion: CTR and ITR, carried out 3 days/week, were efficient to improve endothelium-dependent relaxation and reduce superoxide generation in the aorta from female rats fed with fructose.

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Section: Discussionsupporting

confidence: 67%

Section: Discussionmentioning

confidence: 99%

Interval or continuous aerobic exercise performed 3 days a week increases endothelium-dependent relaxation in female rats fed with fructose

Joaquim

Oharomari

Vale³

et al. 2022

Motriz: rev. educ. fis.

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“…squats), but they are less applicable to studies of BFR due to their inability to generate hindlimb-specific movement, thus potentially introducing compensation by the non-targeted limbs. Other studies that combine BFR and resistance training in rats use low-current neuromuscular electrostimulation in anesthetized rats, which allows for careful control of occlusion pressure but only stimulates contraction of a single muscle 45,47,52 . Further, prolonged exposure to systemic anesthesia can cause reductions in body temperature and impact blood flow through distal vasculature, affecting accurate measurement of blood flow occlusion 53 .…”

Section: Introductionmentioning

confidence: 99%

A Comprehensive Model of Blood Flow Restriction in the Post-surgical Rat

Fosam,

Ramos Nakandakari,

Ohashi

et al. 2024

Preprint

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Blood flow restriction (BFR) with low-load exercise (BFR-exercise) is an increasingly popular tool used to increase muscle strength and attenuate muscle atrophy, especially after injury or surgery. However, the mechanisms underlying BFR-mediated muscle growth are not well understood. Likely contributing to the mechanistic knowledge gap, rodent models of BFR-exercise have not been well described. In this methods paper, we demonstrate a comprehensive, clinically relevant protocol to establish BFR-exercise in awake rats. This protocol includes generating a muscle loss state via bilateral ACL-R, determining targeted blood flow occlusion pressures, and performing weighted hind-limb knee extension exercises with BFR. These methods can be used for further application in mechanistic and physiologic studies of BFR-exercise.

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Low load strength training, associated with or without blood flow restriction increased NO production and decreased production of reactive oxygen species in rats aorta

Cited by 2 publications

References 33 publications

Interval or continuous aerobic exercise performed 3 days a week increases endothelium-dependent relaxation in female rats fed with fructose

Interval or continuous aerobic exercise performed 3 days a week increases endothelium-dependent relaxation in female rats fed with fructose

A Comprehensive Model of Blood Flow Restriction in the Post-surgical Rat

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