Low Level of O2 Inhibits Commitment of Cultured Mesenchymal Stromal Precursor Cells from the Adipose Tissue in Response to Osteogenic Stimuli

Abstract

Mesenchymal stromal precursor cells from human lipoaspirate (lMSC) cultured at 5% O(2) formed 50% less mineralized matrix in response to osteogenic induction than cells cultured under standard conditions (20% O(2)). After lMSC percultured at 5% O(2) were transferred to normoxic conditions (20% O(2)), they produced the same amount of matrix as lMSC permanently cultured at 20% O(2). Hence, hypoxia inhibited the commitment of lMSC under the effect of osteogenic stimuli, which can be important in reparative and regenerative medicine.

Tissue‐Related Hypoxia Attenuates Proinflammatory Effects of Allogeneic PBMCs on Adipose‐Derived Stromal Cells In Vitro

“…Proliferative activity of ASCs was slightly increased during coculture with PBMCs ( Figure 4(b) ). ASC osteogenic capacity revealed by mineralised matrix production was not affected by interaction with PBMCs and was less marked under 5% O 2 both in monoculture and after coculture with PBMCs (Figures 4(c) and 4(d) ), confirming our recent findings [ 25 ].…”

Noninvasive Oxygen Monitoring in Three-Dimensional Tissue Cultures Under Static and Dynamic Culture Conditions

“…Similar observations have been observed by others in the past. 5 , 11 , 13 , 45–48 However, in our dynamic 3D culture system in the collagen scaffold, signs of osteogenic differentiation were found after 4 weeks of static and dynamic culture, despite the lack of osteogenic supplements in the culture medium. In this study, the 3D structure itself together with the matrix material composition of collagen, which is the major structural component of the human musculoskeletal system, might be the main stimulus for spontaneous osteogenic differentiation of the mesenchymal stem cell population.…”

“… 10 The influence of physiological hypoxia (5–10%) on stem cell growth and differentiation is currently a significant research interest. 11–13 On the other hand, an oxygen level at 1% or below can only be tolerated by the cells for a limited time, but can lead toward cell death when prolonged. 14 …”

Resistance of Multipotent Mesenchymal Stromal Cells to Anoxia In Vitro

“…In hypoxia (5% O 2 ) MMSCs demonstrated enhanced growth exceeding that in normoxia (20% O 2 ) in 2.9+0.2 folds (p<0.05) . The osteogenic differentiation capacity of MMSCs was significantly reduced in hypoxia vs normoxia [Grinakovskaya et al, 2009].…”