Low-level light treatment ameliorates immune thrombocytopenia

Abstract

Immune thrombocytopenia (ITP) is an immune-mediated acquired bleeding disorder characterized by abnormally low platelet counts. We reported here the ability of low-level light treatment (LLLT) to alleviate ITP in mice. The treatment is based on noninvasive whole body illumination 30 min a day for a few consecutive days by near infrared light (830 nm) transmitted by an array of light-emitting diodes (LEDs). LLLT significantly lifted the nadir of platelet counts and restored tail bleeding time when applied to two passive ITP models induced by anti-CD41 antibody. The anti-platelet antibody hindered megakaryocyte differentiation from the progenitors, impaired proplatelet and platelet formation, and induced apoptosis of platelets. These adverse effects of anti-CD41 antibody were all mitigated by LLLT to varying degrees, owing to its ability to enhance mitochondrial biogenesis and activity in megakaryocytes and preserve mitochondrial functions in platelets in the presence of the antibody. The observations argue not only for contribution of mitochondrial stress to the pathology of ITP, but also clinical potentials of LLLT as a safe, simple, and cost-effective modality of ITP.

Photobiomodulation of extracellular matrix enzymes in human nucleus pulposus cells as a potential treatment for intervertebral disk degeneration

“…Numerous studies have investigated the effects of PBM in ameliorating or treating various diseases. PBM can lead to reduction of inflammation, cell proliferation, increased synthesis of ATP, and regulation of ECM components 25 – 27 , 35 , 52 – 54 . Low-level light irradiation suppresses the inflammatory response in human adipose-derived stem cells by modulating the activity of cyclic AMP and NF-κB 55 .…”

“…PBM, refers to the use of photons at a non-thermal irradiance to regulate biological activity. Moreover, PBM is efficacious for enabling tissue repair, exerts anti-inflammatory effects, and promotes wound healing in various cells 25 – 35 . During this process, a molecule excited by PBM can interact with a neighboring molecule to enhance photobiological effects, including regulation of a target gene, destruction of enzymes in a catabolic response, and secretion of a specific protein by target cells.…”

Therapeutic potential of intranasal photobiomodulation therapy for neurological and neuropsychiatric disorders: a narrative review

“…In addition to the described changes in tissue oxygenation and modifications of the RBC structure, the systemic effect of nostril-based i-PBMT via irradiation of blood cells could be directly related to the mechanism of hemostasis modulation. On one side, reversible suppression of the sensitivity of platelets to different agonists, and reduced activity of enzymes in the arachidonic acid cascade in a dose-dependent manner of PBMT was reported (Brill et al, 2000;Rola et al, 2017;Drohomirecka et al, 2018) leading to the conclusion that the PBMT could prevent platelets from undergoing apoptosis and prolong their lifespan under pathological conditions (Yang et al, 2016;Zhang et al, 2016Zhang et al, , 2018. Evidence has shown that the mitochondrial respiratory rate and ATP levels are impaired in the blood platelets of patients with depression (Hroudová et al, 2013).…”

Aging of lymphoid organs: Can photobiomodulation reverse age‐associated thymic involution via stimulation of extrapineal melatonin synthesis and bone marrow stem cells?

“…The improvement in heart attack outcome was also shown to work in a large animal model of myocardial infarction in Yorkshire swine [67]. Another group found that non-invasive PBM to the bone marrow could increase the platelet count and ameliorate thrombocytopenia caused by chemotherapy [68] or by an autoimmune disease [69]. …”