Low-Level Laser Therapy Attenuates LPS-Induced Rats Mastitis by Inhibiting Polymorphonuclear Neutrophil Adhesion

Wang, Yueqiang; He, Xianjing; Hao, Dandan; Yu, Dan‐Na; Liang, Jianbin; Qu, Yanpeng; Sun, Dongbo; Yang, Bin; Yang, Keli; Wu, Rui; Wang, Jianfa

doi:10.1292/jvms.14-0061

Cited by 9 publications

(5 citation statements)

References 34 publications

(38 reference statements)

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“…There is a dichotomy that underlies the role of LLLT on inflammation: sometimes, LLLT has pro‐inflammatory effects , while at other times LLLT has mainly anti‐inflammatory effects . The anti‐inflammatory effects are usually manifested as a lesser influx of PMN into a given site of inflammation; for instance, 650 nm LLLT diminished the number of LPS‐induced PMN entering the breast alveoli in a rat model of mastitis , while a 830 nm LLLT protocol was able to significantly reduce the total number of cells and the number of PMN in a model in BALB/c mice in which LPS was used to induce a disease that mimicked acute respiratory distress syndrome (ARDS) . In addition, three out of four doses of 660 nm LLLT significantly reduced myeloperoxidase (MPO) activity in PMN in an ARDS model in mice .…”

Section: Discussionmentioning

confidence: 99%

“…Authors have used different models to study the pro‐inflammatory and anti‐inflammatory responses after LLLT. In order to recruit immune cells to a given anatomical site, some studies have employed chemical irritants such as formaldehyde to induce lung inflammation that mimics inflammation caused by pollutants , LPS to simulate mastitis in rats , or to cause acute respiratory distress syndrome (ARDS) in mice , or have even used live fungal cells to test the fungicidal response of neutrophils . Many reports focus on polymorphonuclear (PMN) cells or neutrophils, as they possess specific receptors that allow them to recognize fungi and bacteria and are one of the principal mediators of the innate immune response against pathogens .…”

Section: Introductionmentioning

confidence: 99%

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Low‐level laser therapy stimulates the oxidative burst in human neutrophils and increases their fungicidal capacity

Cerdeira

Brigagão

Carli

et al. 2016

Journal of Biophotonics

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Low-level laser therapy (LLLT) is known to enhance mitochondrial electron transfer and ATP production; thus, this study asked whether LLLT could stimulate the oxidative burst in human neutrophils (PMN) and improve their ability to kill microorganisms. Blood from healthy human subjects was collected and PMN were isolated from the samples. PMN were treated in vitro with 660 nm or 780 nm CW laser light at 40 mW power and increasing energies up to 19.2 J and were subsequently incubated with Candida albicans cells. Generation of hydroxyl radicals, hypochlorite anions and superoxide anions by PMN were checked using fluorescent probes and chemiluminescence assays; a microbicidal activity assay against C. albicans was also performed. LLLT excited PMN to a higher functional profile, which was translated as superior production of reactive oxygen species (ROS) and increased fungicidal capacity. The most efficacious energy was 19.2 J and, interestingly, the 660 nm light was even more efficacious than 780 nm at increasing the respiratory burst of PMN and the fungicidal capacity. Human neutrophils (PMN) were stimulated in vitro with 660 nm or 780 nm CW laser light at 40 mW of power and a total energy of 19.2 J. Low-level laser therapy (LLLT) excited PMN to a higher functional profile, which was translated as a superior production of reactive oxygen species (ROS) such as hydroxyl radicals (HO•) and hypochlorite anions (ClO−) (Figure) and increased fungicidal capacity against Candida albicans cells.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Low‐level laser therapy stimulates the oxidative burst in human neutrophils and increases their fungicidal capacity

Cerdeira

Brigagão

Carli

et al. 2016

Journal of Biophotonics

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“…TLR4 is activated; it can combine with the molecular chaperone heat shock protein 60, activating downstream nuclear factor κ B (NF- κ B), which finally induces macrophages to produce proinflammatory cytokines [ 12 ]. Such cytokine production is characterized by tumor necrosis factor α (TNF- α ), interleukin-1 β (IL-1 β ), and interleukin-6 (IL-6), which induces neutrophil recruitment, an inflammatory reaction, and tissue damage [ 13 , 14 ]. Penicillin, streptomycin, and ciprofloxacin are commonly used drugs for the treatment of mastitis, but with the frequent use of drugs, drug resistance continues to increase, and its therapeutic effect decreased [ 15 ].…”

Section: Introductionmentioning

confidence: 99%

Anti-Inflammatory Effects of Berberine Hydrochloride in an LPS-Induced Murine Model of Mastitis

Wang

Shi

Ding

et al. 2018

Evidence-Based Complementary and Alternative Medicine

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Berberine hydrochloride is an isoquinoline type alkaloid extracted from Berberidaceae, Rutaceae, and other plants. Previous reports have shown that berberine hydrochloride has anti-inflammatory properties. However, the underlying molecular mechanisms remain unclear. In this study, a lipopolysaccharide- (LPS-) induced murine model of mastitis was established to explore the anti-inflammatory action of berberine hydrochloride. Sixty mice that had been lactating for 5–7 days were randomly divided into six groups, including control, LPS, three berberine hydrochloride treatment groups (5, 10, and 20 mg/kg), and a dexamethasone (DEX) (5 mg/kg) group. Berberine hydrochloride was administered intraperitoneally 1 h before and 12 h after LPS-induced mastitis, and all mice were sacrificed 24 h after LPS induction. The pathological and histopathological changes of the mammary glands were observed. The concentrations and mRNA expressions of TNF-α, IL-1β, and IL-6 were measured by ELISA and qRT-PCR. The activation of TLR4 and NF-κB signaling pathways was analyzed by Western blot. Results indicated that berberine hydrochloride significantly attenuated neutrophil infiltration and dose-dependently decreased the secretion and mRNA expressions of TNF-α, IL-1β, and IL-6 within a certain range. Furthermore, berberine hydrochloride suppressed LPS-induced TLR4 and NF-κB p65 activation and the phosphorylation of I-κB. Berberine hydrochloride can provide mice robust protection from LPS-induced mastitis, potentially via the TLR4 and NF-κB pathway.

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“…10 The photo-biological effects of PBMT and their interference with the immune system are very well known. [11][12][13][14] Studies also reported the effect of PBMT on cytokine (IL-4, IL-10, or IL-13) and chemokine (CCL2, CXCL10, and TNF-α) production and elucidate the mechanism via histone modification of cytokine expression in monocytes, as well as enhanced cytokine and chemokine expression in mRNA and protein levels. 15 The effect of PBMT on proinflammatory cytokines or other mediators and their important role in the pathophysiology of inflammatory diseases such as osteoarthritis (OA) has also been reported.…”

Section: Introductionmentioning

confidence: 99%

The Probable Protective Effect of Photobiomodulation on the Immunologic Factor’s mRNA Expression Level in the Lung: An Extended COVID-19 Preclinical and Clinical Meta-analysis

Arjmand

Rahim

2023

Clin Med�Insights�Pathol

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Background: Different expression of cytokine genes in the body determines the type of immune response (Th1 or Th2), which can play an important role in the pathogenesis of the COVID-19 disease. Aims: This meta-analysis was conducted to evaluate the probable effect of photobiomodulation (PBMT) therapy on the cytokine’s mRNA expression in the lung. Methods: We systematically searched indexing databases, including PubMed/Medline, ISI web of science, Scopus, EMBASE, and Cochrane central, using standard terms without language, study region or type restrictions. Studies on using PBM in lung injury modeling with samples collected from lung tissue to observe IL-1β, TNF-α, IL-10, and IL-6 mRNA expression were included. RevMan 5.3 software was used for data analysis and standardized mean difference as effect size. Results: Of the 438 studies found through initial searches, 17 met the inclusion criteria. The main properties of 13 articles on 384 animals included in this meta-analysis with a wide range of species include rats (n = 10) and rabbits (n = 3). The analysis revealed that PBMT reduced the mRNA expression of TNFα (SMD: −3.70, 95% CI: −6.29, −1.11, P = .005, I2 = 71%) and IL-1β (SMD: −5.85, 95% CI: −8.01, −3.69, P < .00001, I2 = 37%) significantly, but no statistically significant reduction in IL-6 (SMD: −2.89, 95% CI: −5.79, 0.01, P = .05, I2 = 88%) was observed compared with the model controls. Also, PBMT increased IL-10 mRNA expression significantly compared with the model controls (SMD: 1.04, 95% CI: 0.43, 1.64, P = .0008, I2 = 17%). Conclusion: This meta-analysis revealed that the PBMT utilizes beneficial anti-inflammatory effects and modulation of the immune system on lung damage in animal models and clinical studies. However, animal models and clinical studies appear limited considering the evidence’s quality; therefore, large clinical trials are still required.

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Low-Level Laser Therapy Attenuates LPS-Induced Rats Mastitis by Inhibiting Polymorphonuclear Neutrophil Adhesion

Cited by 9 publications

References 34 publications

Low‐level laser therapy stimulates the oxidative burst in human neutrophils and increases their fungicidal capacity

Low‐level laser therapy stimulates the oxidative burst in human neutrophils and increases their fungicidal capacity

Anti-Inflammatory Effects of Berberine Hydrochloride in an LPS-Induced Murine Model of Mastitis

The Probable Protective Effect of Photobiomodulation on the Immunologic Factor’s mRNA Expression Level in the Lung: An Extended COVID-19 Preclinical and Clinical Meta-analysis

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