Anti‐Inflammatory Effects of Berberine Hydrochloride in an LPS‐Induced Murine Model of Mastitis

Wang, Xichun; Shi, Feng; Ding, Nana; Yang, He; Li, Cheng; Li, Manman; Ding, Xuedong; Ding, Hongyan; Li, Jinchun; Wu, Jiawei; Li, Yu

doi:10.1155/2018/5164314

Cited by 29 publications

(22 citation statements)

References 24 publications

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“…4), which suggest that BBR has anti-inflammatory effects mainly through NF-κB deactivation in LPS-exposed mIMCD-3 cells. Some previous researches have reported that BBR inhibits inflammatory mediators and NF-κB signaling in various different disease models (19,(63)(64)(65)(66)(67). However, there is no report that BBR could have anti-inflammatory and protective effects in lPS-exposed inner medullary collecting duct (iMcd) cells.…”

Section: Discussionmentioning

confidence: 99%

Berberine ameliorates lipopolysaccharide‑induced inflammatory responses in mouse inner medullary collecting duct‑3 cells by downregulation of NF‑κB pathway

Kim

Choi

et al. 2019

Mol Med Report

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The major role of inner medullary collecting duct (iMcd) cells is to maintain water and sodium homeostasis. in addition to the major role, it also participates in the protection of renal and systemic inflammation. Although IMCD cells could take part in renal and systemic inflammation, investigations on renal inflammation in IMCD cells have rarely been reported. Although berberine (BBR) has been reported to show diverse pharmacological effects, its anti-inflammatory and protective effects on IMCD cells have not been studied. Therefore, in the present study, we examined the anti-inflammatory and protective effects of BBR in mouse IMCD-3 (mIMCD-3) cells against lipopolysaccharide (lPS). an MTT assay was carried out to investigate the toxicity of BBR on mIMCD-3 cells. Reverse transcription quantitative-PCR and western blotting were performed to analysis pro-inflammatory molecules and cytokines. Mechanisms of BBR were examined by western blotting and immunocytochemistry. According to previous studies, pro-inflammatory molecules, such as inducible nitric oxide synthase and cyclooxygenase-2, and pro-inflammatory cytokines, such as interleukin (il)-1β, il-6 and tumor necrosis factor-α are increased in LPS-exposed mIMCD-3 cells. However, the production of these pro-inflammatory molecules is significantly inhibited by treatment with BBR. In addition, BBR inhibited translocation of nuclear factor (NF)-κB p65 from the cytosol to the nucleus, and degradation of inhibitory κ-Bα in LPS-exposed mIMCD-3 cells. In conclusion, BBR could inhibit renal inflammatory responses via inhibition of NF-κB signaling and ultimately contribute to amelioration of renal injury during systemic inflammation.

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Section: Discussionmentioning

confidence: 99%

Berberine ameliorates lipopolysaccharide‑induced inflammatory responses in mouse inner medullary collecting duct‑3 cells by downregulation of NF‑κB pathway

Kim

Choi

et al. 2019

Mol Med Report

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“…Firstly, the major compounds of CBT were identified by HPLC, and it was confirmed that it contains crocin (28.671 mg/g), berberine hydrochloride (6.255 mg/g), and glycyrrhizic acid (6.249 mg/g). Previous studies have reported these compounds have anti-inflammatory and anti-oxidant effects (Fagot et al, 2018;Wang et al, 2018).…”

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confidence: 99%

Chijabyukpi-Tang Inhibits Pro-Inflammatory Cytokines and Chemokines via the Nrf2/HO-1 Signaling Pathway in TNF-α/IFN-γ-Stimulated HaCaT Cells and Ameliorates 2,4-Dinitrochlorobenzene-Induced Atopic Dermatitis-Like Skin Lesions in Mice

Lee

Lim

et al. 2020

Front. Pharmacol.

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Chijabyukpi-tang (CBT) is an oriental herbal formula consisting of three herbs (Gardeniae Fructus (Gardenia jasminoides J.Ellis.), Phellodendri Cortex (Phellodendron amurense Rupr.), Glycyrrhizae Radix (Glycyrrhiza uralensis Fisch. ex DC.) at the ratio of 2: 2: 1. CBT has traditionally been used to treat eczema with inflammation in Northeast Asia. The components of CBT have been shown to have anti-inflammatory and anti-oxidant properties, but the exact role and mechanism of CBT on atopic dermatitis (AD) remain unclear. In this study, we investigated the anti-inflammatory effect and mechanism of CBT in the HaCaT human keratinocyte cell line and investigated the anti-atopic effect in mice models of atopic dermatitis-like skin lesions. In the tumor necrosis factor alpha (TNF)-a/ interferon (IFN)-g-stimulated HaCaT cells, CBT inhibited the production of proinflammatory cytokines and chemokines and elevated the nuclear translocation of NF-E2 p45 related factors 2 (Nrf2) and subsequent production of heme oxygenase-1 (HO-1). CBT improved the symptoms of atopic dermatitis-like lesions in 2,4-dinitrochlorobenzene (DNCB)-treated mice by suppressing the levels of serum immunoglobulin E (IgE), and various pro-inflammatory cytokines and chemokines. The improvement effect of CBT on atopic dermatitis-like lesions can be predicted to be due to increased Nrf2 and HO-1 gene expression. These results suggest that CBT is an herbal medicine with the potential for use as a therapeutic agent for inflammatory skin diseases such as atopic dermatitis.

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“…BBRH has been widely used as a therapeutic agent for infections and heart failure (Fu et al 2015) due to its antiviral, antibacterial, and blood pressure-reducing activities. It was previously demonstrated that BBRH could be effectively used against LPS-triggered endometritis in mice (Wang et al 2018). Neutrophil infiltration and MPO function was repressed by BBRH via NFκB stimulation (Shao et al 2015).…”

Section: Discussionmentioning

confidence: 98%

Berberine hydrochloride mitigates acute pancreatitis by suppressing the TLR4/IκBα/NFκB pathway

Liu

et al. 2020

All Life

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Severe acute pancreatitis (SAP) usually causes multiple-organ dysfunction syndrome and exhibits a high mortality rate. It has been previously reported that berberine, as a dominant alkaloid, can be effectively used against inflammation. However, there is limited data on the effects of berberine hydrochloride (BBRH) on inflammation in SAP rat models. Male Sprague-Dawley rats were given preliminary intraperitoneal injections of BBRH 30 min prior to retrograde infusion of 5% sodium taurocholate in their biliopancreatic ducts. The rats were sacrificed 12 h after taurocholate administration, and tissues and blood samples were then acquired for histological, chemical, and molecular analyses. The SAP models exhibited enhanced edema, ascitic fluid volume, neutrophil infiltration, serum amylase and lipase levels, and interleukin (IL)-6, tumor necrosis factor-α, and IL-1β levels, and reduced IL-10 concentration, while BBRH administration reversed these changes. Further study showed that in the SAP model, protein and mRNA levels of nuclear factor kappa-light-chainenhancer of activated B (NFκB) p65 and toll-like receptor 4 (TLR4) were increased and inhibitor of nuclear factor kappa B (IκBα) expression was decreased, while pretreatment with BBRH significantly decreased the activation of the TLR4/IκBα/NFκB pathway. The data obtained displayed that BBRH mitigated acute pancreatitis by suppressing the TLR4/IκB/NFκB pathway.

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Anti‐Inflammatory Effects of Berberine Hydrochloride in an LPS‐Induced Murine Model of Mastitis

Cited by 29 publications

References 24 publications

Berberine ameliorates lipopolysaccharide‑induced inflammatory responses in mouse inner medullary collecting duct‑3 cells by downregulation of NF‑κB pathway

Berberine ameliorates lipopolysaccharide‑induced inflammatory responses in mouse inner medullary collecting duct‑3 cells by downregulation of NF‑κB pathway

Chijabyukpi-Tang Inhibits Pro-Inflammatory Cytokines and Chemokines via the Nrf2/HO-1 Signaling Pathway in TNF-α/IFN-γ-Stimulated HaCaT Cells and Ameliorates 2,4-Dinitrochlorobenzene-Induced Atopic Dermatitis-Like Skin Lesions in Mice

Berberine hydrochloride mitigates acute pancreatitis by suppressing the TLR4/IκBα/NFκB pathway

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