Low-level bisphenol A increases production of glial fibrillary acidic protein in differentiating astrocyte progenitor cells through excessive STAT3 and Smad1 activation

Yamaguchi, Hideaki; Jing, Zhu; Tang, Yu; Sasaki, Kazuo; Umetsu, Hironori; Kidachi, Yumi; Ryoyama, Kazuo

doi:10.1016/j.tox.2006.06.011

Cited by 17 publications

(26 citation statements)

References 44 publications

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“…The observation that ntC values in Gliacultures superseded those measured in ntC Glia+ suggests that the glia plays a role in the regulation of transcription, regardless of the type of receptor examined; even when cells were only treated with BPA alone, consequential decrease in TR transcription was more evident if the glia was present in the culture. While these observations are in concordance with previous results (Yamaguchi et al, 2006;Fauquier et al, 2014), our data also show that the effects of BPA on TR transcription are multifactorial and, in addition, differ depending on the presence or the absence of glia.…”

Section: Receptor Mrna Levelssupporting

confidence: 94%

Bisphenol A influences oestrogen- and thyroid hormone-regulated thyroid hormone receptor expression in rat cerebellar cell culture

Somogyi

Horváth

Tóth

et al. 2016

Acta Veterinaria Hungarica

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Thyroid hormones (THs) and oestrogens are crucial in the regulation of cerebellar development. TH receptors (TRs) mediate these hormone effects and are regulated by both hormone families. We reported earlier that THs and oestradiol (E 2 ) determine TR levels in cerebellar cell culture. Here we demonstrate the effects of low concentrations (10 -10 M) of the endocrine disruptor (ED) bisphenol A (BPA) on the hormonal (THs, E 2 ) regulation of TRα,β in rat cerebellar cell culture. Primary cerebellar cell cultures, glia-containing and glia-destroyed, were treated with BPA or a combination of BPA and E 2 and/or THs. Oestrogen receptor and TH receptor mRNA and protein levels were determined by real-time qPCR and Western blot techniques. The results show that BPA alone decreases, while BPA in combination with THs and/or E 2 increases TR mRNA expression. In contrast, BPA alone increased receptor protein expressions, but did not further increase them in combination with THs and/or E 2 . The modulatory effects of BPA were mediated by the glia; however, the degree of changes also depended on the specific hormone ligand used. The results signify the importance of the regulatory mechanisms interposed between transcription and translation and raise the possibility that BPA could act to influence nuclear hormone receptor levels independently of ligand-receptor interaction.Key words: Endocrine disruptors, thyroid receptor, transcription, translation, bisphenol A, rat cerebellum Thyroid hormones (THs) and oestrogens (mostly 17β-oestradiol, E 2 ) play critical roles in the regulation of central nervous system (CNS) development, in-* Corresponding author; Zsarnovszky.Attila@mkk.szie.hu; Phone: 0036 (28) 522-062/1620 (office); 0036 (30) 665-3717 (mobile); Fax: 0036 (28) 522-062

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Section: Receptor Mrna Levelssupporting

confidence: 94%

Bisphenol A influences oestrogen- and thyroid hormone-regulated thyroid hormone receptor expression in rat cerebellar cell culture

Somogyi

Horváth

Tóth

et al. 2016

Acta Veterinaria Hungarica

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“…SFME cells were originally derived from a 16-d-old whole mouse embryo 29) and have been documented to increase GFAP, the astrocyte intermediate filament marker protein, following the addition of serum, TGF-b, 56,57) LIF 58) or BMPs 59) or following the addition of LIF plus BMP2. 60) These cells offer advantages for studying cell differentiation because they serve as progenitor cells and proliferate without senescence in vitro, 34,59) and have long been considered to differentiate into only an astrocytic lineage. Our present study showed that the cells express early neural, neuronal and oligodendrocytic markers, as well as the astrocytic marker.…”

Section: Resultsmentioning

confidence: 99%

Differentiation of Serum-Free Mouse Embryo Cells into an Astrocytic Lineage is Associated with the Asymmetric Production of Early Neural, Neuronal and Glial Markers

Yamaguchi

Kidachi

Umetsu

et al. 2008

Biological & Pharmaceutical Bulletin

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Serum-free mouse embryo (SFME) cells, the astrocyte progenitor cells in the central nervous system (CNS), were exposed to 10 ng/ml leukemia inhibitory factor (LIF) and 10 ng/ml bone morphogenic protein 2 (BMP2) to induce differentiation, and expression of cell-type specific markers. Nestin, a marker of early neural lineage, b bIII-tubulin, a marker of neuronal lineage, oligodendrocyte marker O4 (O4), a marker of oligodendrocytic lineage and glial fibrillary acidic protein (GFAP), a marker of astrocytic lineage, were analyzed. Characteristics of SFME cells, as a CNS progenitor, were identified and a possible mechanism, underlying SFME cell specification into an astrocytic lineage upon differentiation, was investigated. These markers were present, both at the initial proliferative phase and after induction of differentiation. GFAP expression increased strongly upon differentiation, while expression of the other markers changed very little. These results indicate that astrocytic differentiation is associated with the asymmetric production of these markers, rather than through induction of astrocytic markers.

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“…Although the study contains suggestive mechanistic information, it is not useful for the evaluation process. Yamaguchi et al (2006), supported by the Promotion and Mutual Aid Corporation for Private Schools of Japan, examined the effects of low-level bisphenol A exposure on the differentiation of serum-free mouse embryo astrocyte progenitor cells into astrocytes. Astrocyte progenitor cells were grown on fibronectin-coated Petri dishes under standard incubator conditions.…”

Section: Utility (Adequacy) For Cerhr Evaluation Processmentioning

confidence: 99%

NTP‐CERHR expert panel report on the reproductive and developmental toxicity of bisphenol A

Chapin

Adams

Boekelheide

et al. 2008

Birth Defects Research Pt B

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Low-level bisphenol A increases production of glial fibrillary acidic protein in differentiating astrocyte progenitor cells through excessive STAT3 and Smad1 activation

Cited by 17 publications

References 44 publications

Bisphenol A influences oestrogen- and thyroid hormone-regulated thyroid hormone receptor expression in rat cerebellar cell culture

Bisphenol A influences oestrogen- and thyroid hormone-regulated thyroid hormone receptor expression in rat cerebellar cell culture

Differentiation of Serum-Free Mouse Embryo Cells into an Astrocytic Lineage is Associated with the Asymmetric Production of Early Neural, Neuronal and Glial Markers

NTP‐CERHR expert panel report on the reproductive and developmental toxicity of bisphenol A

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