Low intestinal colonization of Escherichia coli clone ST131 producing CTX-M-15 in Jordanian infants

Abstract

Over a period of 3 years' study (2012)(2013)(2014), a total of 518 faecal samples were collected and cultured to isolate Escherichia coli. Of these, 338 (65.3 %) E. coli isolates were recovered from infants, and 142/338 (42 %) were multidrug-resistant (MDR) to $3 drug classes using the antimicrobial susceptibility disc diffusion method. A total of 125/142 (88 %) of E. coli isolates were extended-spectrum b-lactamase (ESBL) producers. blaCTX-M-15 types were observed in 80/125 (64 %) of the isolates, and 60/80 (75 %) were positive for blaCTX-M-15. Out of 338 E. coli isolates, 9 (2.6 %) were positive for ST131/O25b clone and each isolate was associated with several plasmids of different sizes (1-21.2 kb). The identities of these nine isolates were confirmed by sequencing for presence of pabB (347 bp) and trpA (427 bp) genes. This study demonstrates low prevalence rate of the highly virulent E. coli ST131 clone producing blaCTX-M-15 in the intestines of Jordanian infants.

Investigating possible association between multidrug resistance and isolate origin with some virulence factors ofEscherichia colistrains isolated from infant faeces and fresh green vegetables

“…Escherichia coli faecal strains (68 strains) were isolated from healthy infants (<1 year old). The isolates were collected, isolated and identified in a previous work (Badran et al 2016). In that work, the collection of the isolates was performed after obtaining the necessary ethical approval and consent forms (Badran et al 2016).…”

“…The isolates were collected, isolated and identified in a previous work (Badran et al 2016). In that work, the collection of the isolates was performed after obtaining the necessary ethical approval and consent forms (Badran et al 2016). Escherichia coli Nissle 1917, a probiotic nonpathogenic microorganism was the kind gift of Ardeypharm GmbH (Herdecke, Germany).…”

“…Escherichia coli-specific gene yccT was used as an internal standard (Clifford et al 2012). This gene was chosen to confirm the identity of E. coli isolates that were obtained from other studies (Burjaq and Shehabi 2013;Badran et al 2016) as mentioned earlier, in addition to its role as an internal standard. The reaction mixture (20 ll) contained the following: DNA (2 ll), 400 nmol l À1 final concentration of ST primers (200 nmol l À1 for siderophores, LT, yccT), 10 ll KAPA SYBR Fast â Universal qPCR (KAPA Biosystems, Wilmington, MA).…”

High incidence of multidrug-resistant fecal E. coli producing ESBLs and carried ST131 in Jordanian adults

“…Class of CTX-M type enzymes are the most prevalent extended-spectrum β-lactamases (ESBLs) worldwide including Arab Middle East countries [2,3]. Among the CTX-M type enzymes, CTX-M-15 has been frequently found in Arab countries over the last decade [3][4][5][6][7].…”

“…This new discovered ST131 clone has been recently isolated from a diverse range of infections caused in community and hospitalized patients across the world, and it has been found as ESBL CTX-M-15-producer with a high virulence potential. In addition, ST131 clone has been reported to cause major infection complications from cystitis to lifethreatening sepsis in many countries [4,[12][13][14]. Fluoroquinolones and trimethoprim are no longer effective for empiric therapy of E. coli ST131 infections [13][14][15][16].…”