Low intensity pulsed ultrasound promotes the migration of bone marrow- derived mesenchymal stem cells via activating FAK-ERK1/2 signalling pathway

Jiang, Jingwei; Wang, Wei; Qin, Juan; Chen, Jinyun; Chen, Wenzhi; Wang, Yan

doi:10.1080/21691401.2019.1657878

Cited by 30 publications

(25 citation statements)

References 39 publications

(35 reference statements)

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“…MSC migration in vitro can be enhanced by LIPUS, and this phenomenon has been associated with increases in CXCR4, integrin-1β [ 49 ] and CCR-2 [ 42 ] expression, and also with cytoskeletal rearrangements involving the focal adhesion kinase (FAK)-ERK1/2 signaling pathway [ 50 ]. LIPUS treatment increases the migration of periodontal ligament stem cells (PDLSC) concomitant with an upregulation of TWIST1 and SDF-1, suggesting the involvement of the SDF1/CXCR4 signaling axis [ 51 ].…”

Section: Biological Effects Of Ultrasoundmentioning

confidence: 99%

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Ultrasound Therapy: Experiences and Perspectives for Regenerative Medicine

Lucas

Pérez

Bernal

et al. 2020

Genes

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Ultrasound has emerged as a novel tool for clinical applications, particularly in the context of regenerative medicine. Due to its unique physico-mechanical properties, low-intensity ultrasound (LIUS) has been approved for accelerated fracture healing and for the treatment of established non-union, but its utility has extended beyond tissue engineering to other fields, including cell regeneration. Cells and tissues respond to acoustic ultrasound by switching on genetic repair circuits, triggering a cascade of molecular signals that promote cell proliferation, adhesion, migration, differentiation, and extracellular matrix production. LIUS also induces angiogenesis and tissue regeneration and has anti-inflammatory and anti-degenerative effects. Accordingly, the potential application of ultrasound for tissue repair/regeneration has been tested in several studies as a stand-alone treatment and, more recently, as an adjunct to cell-based therapies. For example, ultrasound has been proposed to improve stem cell homing to target tissues due to its ability to create a transitional and local gradient of cytokines and chemokines. In this review, we provide an overview of the many applications of ultrasound in clinical medicine, with a focus on its value as an adjunct to cell-based interventions. Finally, we discuss the various preclinical and clinical studies that have investigated the potential of ultrasound for regenerative medicine.

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Section: Biological Effects Of Ultrasoundmentioning

confidence: 99%

“…Ultrasound has great potential to enhance the recruitment and migration of stem cells to target tissue [ 50 ]. In the context of bone fractures, LIPUS induced the recruitment of MSC to the injury site, which resulted in improved callus formation and microarchitecture likely mediated by SDF-1 [ 54 ].…”

Section: Biological Effects Of Ultrasoundmentioning

confidence: 99%

Ultrasound Therapy: Experiences and Perspectives for Regenerative Medicine

Lucas

Pérez

Bernal

et al. 2020

Genes

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“…While the present study did not investigate the mechanisms underlying this effect, possible pathways include the mitogen-activated protein kinase 47 , focal adhesion kinase activates-extracellular signal-regulated kinase 1/2 48 , and stromal cell-derived factor-1/C-X-C chemokine receptor type 4 49 . These pathways have already been shown to be activated following stimulation of stem cells with low-intensity pulsed ultrasound (LIPUS) [47][48][49] . Future work will aim to investigate these mechanisms systemically and the effects of different acoustic parameters on these pathways, in order to better determine how to effectively modulate the function of MSCs.…”

Section: Discussionmentioning

confidence: 93%

The Paracrine Function of Mesenchymal Stem Cells in Response to Pulsed Focused Ultrasound

et al. 2020

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We studied the paracrine function of mesenchymal stem cells (MSCs) derived from various sources in response to pulsed focused ultrasound (pFUS). Human adipose tissue (AD), bone marrow (BM), and umbilical cord (UC) derived MSCs were exposed to pFUS at two intensities: 0.45 W/cm2 ISATA (310 kPa PNP) and 1.3 W/cm2 ISATA (540 kPa PNP). Following pFUS, the viability and proliferation of MSCs were assessed using a hemocytometer and confocal microscopy, and their secreted cytokine profile determined using a multiplex ELISA. Our findings showed that pFUS can stimulate the production of immunomodulatory, anti-inflammatory, and angiogenic cytokines from MSCs which was dependent on both the source of MSC being studied and the acoustic intensity employed. These important findings set the foundation for additional mechanistic and validation studies using this novel noninvasive and clinically translatable technology for modulating MSC biology.

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“…Therefore, a safer, more effective As an emerging physical therapy, low-intensity pulsed ultrasound (LIPUS) has been approved as a safe noninvasive method for the treatment of bone healing, softtissue regeneration, inhibition of inflammation, noninvasive neuromodulation, and other applications (13). Our previous experimental study showed that LIPUS can promote the proliferation, differentiation and migration of bone marrow mesenchymal stem cells (14)(15)(16), and also found that LIPUS can treat leukopenia caused by cyclophosphamide chemotherapy and elevate leukocytes count after cytarabine chemotherapy (17,18).…”

Section: Original Articlementioning

confidence: 99%

Low-intensity pulsed ultrasound alleviating myelosuppression of Sprague-Dawley rats after combined treating by paclitaxel and carboplatin

Luo¹,

Chen²,

Wang³

et al. 2021

Transl Cancer Res TCR

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Background: Our team has previously reported that low intensity pulsed ultrasound (LIPUS) can alleviate myelosuppression in rats induced by single chemotherapy drugs. But in clinics, chemotherapy is often performed with multiple drugs simultaneously. To be closer to the clinical status quo, this experiment was designed to show whether it was the same effect of LIPUS on myelosuppression caused by combination therapy of chemotherapy drugs.Methods: The rat model of myelosuppression was established by continuous injection of paclitaxel and carboplatin for 4 days. These myelosuppressive rats were randomly divided into LIPUS group (n=40) and control group (n=40). The LIPUS group was given continuous LIPUS irradiation for 7 days, while the control group was given sham irradiation (no energy output). The evaluation of blood cells counts, Hematoxylin-Eosin staining (H&E staining), scanning electron microscopy, enzyme-linked immunosorbent assay (ELISA) and real-time quantitative PCR (qPCR) was then performed. Results:The results showed in the LIPUS group the blood cells count, hematopoietic tissue of bone marrow, the colonies formed from adhering of bone marrow stromal cells, levels of hematopoietic regulators and adhesion molecules all increased (LIPUS group vs. control group, P<0.05). Conclusions:The results indicated that LIPUS can relieve myelosuppression induced by combined treatment of paclitaxel and carboplatin. The mechanisms may be LIPUS can increase the levels of hematopoietic regulators and adhesion molecules.

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Low intensity pulsed ultrasound promotes the migration of bone marrow- derived mesenchymal stem cells via activating FAK-ERK1/2 signalling pathway

Abstract: (2019) Low intensity pulsed ultrasound promotes the migration of bone marrowderived mesenchymal stem cells via activating FAK-ERK1/2 signalling pathway, Artificial Cells,

Cited by 30 publications

References 39 publications

Ultrasound Therapy: Experiences and Perspectives for Regenerative Medicine

Ultrasound Therapy: Experiences and Perspectives for Regenerative Medicine

The Paracrine Function of Mesenchymal Stem Cells in Response to Pulsed Focused Ultrasound

Low-intensity pulsed ultrasound alleviating myelosuppression of Sprague-Dawley rats after combined treating by paclitaxel and carboplatin

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