Low-intensity pulsed ultrasound alleviating myelosuppression of Sprague-Dawley rats after combined treating by paclitaxel and carboplatin

Abstract

Background: Our team has previously reported that low intensity pulsed ultrasound (LIPUS) can alleviate myelosuppression in rats induced by single chemotherapy drugs. But in clinics, chemotherapy is often performed with multiple drugs simultaneously. To be closer to the clinical status quo, this experiment was designed to show whether it was the same effect of LIPUS on myelosuppression caused by combination therapy of chemotherapy drugs.Methods: The rat model of myelosuppression was established by continuous injection of paclitaxel and carboplatin for 4 days. These myelosuppressive rats were randomly divided into LIPUS group (n=40) and control group (n=40). The LIPUS group was given continuous LIPUS irradiation for 7 days, while the control group was given sham irradiation (no energy output). The evaluation of blood cells counts, Hematoxylin-Eosin staining (H&E staining), scanning electron microscopy, enzyme-linked immunosorbent assay (ELISA) and real-time quantitative PCR (qPCR) was then performed. Results:The results showed in the LIPUS group the blood cells count, hematopoietic tissue of bone marrow, the colonies formed from adhering of bone marrow stromal cells, levels of hematopoietic regulators and adhesion molecules all increased (LIPUS group vs. control group, P<0.05). Conclusions:The results indicated that LIPUS can relieve myelosuppression induced by combined treatment of paclitaxel and carboplatin. The mechanisms may be LIPUS can increase the levels of hematopoietic regulators and adhesion molecules.

Enhancing the therapeutic efficacy of gefitinib on subcutaneously transplanted SKOV3 ovarian cancer tumors in nude mice via ultrasound‑stimulated microbubble cavitation

“…Notably, low-intensity ultrasound has been shown to suppress lipopolysaccharide-induced inflammatory responses by inhibiting TLR4 signal transduction. Therefore, low-intensity ultrasound may have biological effects in different tissues by affecting various signaling pathways (12)(13)(14)(15). A previous study reported that low-intensity ultrasound may reduce the phosphorylation of STAT3 in ovarian cancer stem cells through the interleukin 6 (IL-6)/STAT3 pathway (16).…”

Human Serum Albumin Nanoparticles as a Carrier of 20(S)-Protopanaxadiol via Intramuscular Injection to Alleviate Cyclophosphamide-Induced Myelosuppression

“…Myelosuppression caused by chemotherapy, as reflected by decreased numbers of red blood cells, white blood cells (WBCs), and platelets according to the National Cancer Institute, can affect the quality of life and prognosis of patients. Once myelosuppression is induced, patients will be at risk of anemia, hemorrhage, or fatal infections. − Unfortunately, complications may not only interrupt the course of chemotherapy or radiotherapy but also impede the therapeutic outcome. Currently, hematopoietic growth factors like granulocyte colony growth factors (G-CSFs), sargramostim (GM-CSF), and erythropoietin stimulating agents are used to regulate the hematopoietic function but they are expensive and are associated with adverse effects like bone pain and the risk of myocardial infarction, heart failure, stroke, and thrombosis …”