Low-intensity pulsed ultrasound (LIPUS) stimulates mineralization of MC3T3-E1 cells through calcium and phosphate uptake

Tassinary, João Alberto Fioravante; Lunardelli, Adroaldo; Basso, Bruno de Souza; Dias, Henrique Bregolin; Catarina, Anderson Velasque; Stülp, Simone; Haute, Gabriela Viegas; Martha, Bianca Andrade; Melo, Denizar Alberto da Silva; Nunes, Fernanda Bordignon; Donadio, Márcio Vinı́cius Fagundes; Oliveira, Jarbas Rodrigues de

doi:10.1016/j.ultras.2017.11.011

Cited by 24 publications

(16 citation statements)

References 40 publications

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“…Similarly, the mechanism of LIPUS-induced bone regeneration is also associated with ions uptake and angiogenesis. Tassinary et al 29 proved that LIPUS was capable of stimulating differentiation and mineralization of MC3T3-E1 cells through Ca 2+ and PO 4 3uptake. Meanwhile, LIPUS can increase the mRNA level of vascular endothelial growth factor A 26 .…”

Section: Discussionmentioning

confidence: 99%

Does low-intensity pulsed ultrasound accelerate phasic calcium phosphate ceramic-induced bone formation?

et al. 2023

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Purpose: Low-intensity pulsed ultrasound (LIPUS) has been used to stimulate the healing of the fresh fracture, delayed union, and non-union in both animal and clinical studies. Besides, biphasic calcium phosphate ceramic (BCP) is a promising biomaterial for bone repair as it shows favorable biocompatibility, osteoinduction, and osteoconduction. However, scarcity is known about the combined effect of LIPUS and BCP on bone formation. Methods: The combined effect of LIPUS and BCP was studied in a beagle model. Twelve dogs were used. BCP granules without any additions were implanted into bilateral erector spinae muscles. One side is the BCP group, while the counterlateral side is LIPUS + BCP group. Histological and histomorphometric analyses, and quantitative real-time polymerase chain reaction were evaluated. Results: Compared with BCP alone, the LIPUS + BCP showed no advantages in early bone formation. Furthermore, the Notch signaling pathway-related mRNA has no significant difference between the two groups. Conclusions: The preliminary results showed that the BCP, which has intrinsic osteoinduction nature, was an effective and promising material. However, LIPUS has no enhanced effect in BCP induced ectopic bone formation. Furthermore, LIPUS has no effect on the Notch signaling pathway. Whether costly LIPUS could be used in combination with BCP should be a rethink.

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Section: Discussionmentioning

confidence: 99%

Does low-intensity pulsed ultrasound accelerate phasic calcium phosphate ceramic-induced bone formation?

et al. 2023

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“…The primary mechanisms by which LIPUS is believed to stimulate osseous formation are summarized in Table 3, 45,47–61 as well as conceptually illustrated in Figure 4. Pressure waves produced by LIPUS in biological tissues are detected by integrins, which translate the biomechanical input to biochemical signaling cascades.…”

Section: Discussionmentioning

confidence: 99%

Low‐Intensity Pulsed Ultrasound as a Potential Adjuvant Therapy to Promote Spinal Fusion

Cottrill

Downey

Pennington

et al. 2020

J of Ultrasound Medicine

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Despite extensive research, nonunion continues to affect a nontrivial proportion of patients undergoing spinal fusion. Recently, preclinical studies have suggested that low‐intensity pulsed ultrasound (LIPUS) may increase rates of spinal fusion. In this study, we summarized the available in vivo literature evaluating the effect of LIPUS on spinal fusion and performed a meta‐analysis of the available data to estimate the degree to which LIPUS may mediate higher fusion rates. Across 13 preclinical studies, LIPUS was associated with a 9‐fold increase in the odds of successful spinal fusion. Future studies are necessary to establish the benefit of LIPUS on spinal fusion in clinical populations.

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“…As extensively shown in vitro studies, LIPUS increases the expression of genes related to bone formation, such as Runx-2, osteocalcin, TGF-β, collagen type I, and X, alkaline phosphatase (ALP), aggrecan, Insulin-like growth factor-1 (IGF-1) and bone sulfur protein (Pan et al, 2019b;He et al, 2021;Maung et al, 2021). Apart from that, LIPUS has also been described to promote protein synthesis and calcium absorption in different osteoblast cell lines (Tassinary et al, 2018). Mechanotransduction is the mechanism that has been put forward to explain the process where the mechanical signal is converted into a biological signal.…”

Section: Lipus In Bone Regeneration Therapymentioning

confidence: 99%

LIPUS as a potential strategy for periodontitis treatment: A review of the mechanisms

Aimaijiang

Liu²,

Zhang³

et al. 2023

Front. Bioeng. Biotechnol.

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Periodontitis is a chronic inflammatory condition triggered by oral bacteria. A sustained inflammatory state in periodontitis could eventually destroy the alveolar bone. The key objective of periodontal therapy is to terminate the inflammatory process and reconstruct the periodontal tissues. The traditional Guided tissue regeneration (GTR) procedure has unstable results due to multiple factors such as the inflammatory environment, the immune response caused by the implant, and the operator’s technique. Low-intensity pulsed ultrasound (LIPUS), as acoustic energy, transmits the mechanical signals to the target tissue to provide non-invasive physical stimulation. LIPUS has positive effects in promoting bone regeneration, soft-tissue regeneration, inflammation inhibition, and neuromodulation. LIPUS can maintain and regenerate alveolar bone during an inflammatory state by suppressing the expression of inflammatory factors. LIPUS also affects the cellular behavior of periodontal ligament cells (PDLCs), thereby protecting the regenerative potential of bone tissue in an inflammatory state. However, the underlying mechanisms of the LIPUS therapy are still yet to be summarized. The goal of this review is to outline the potential cellular and molecular mechanisms of periodontitis-related LIPUS therapy, as well as to explain how LIPUS manages to transmit mechanical stimulation into the signaling pathway to achieve inflammatory control and periodontal bone regeneration.

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Low-intensity pulsed ultrasound (LIPUS) stimulates mineralization of MC3T3-E1 cells through calcium and phosphate uptake

Cited by 24 publications

References 40 publications

Does low-intensity pulsed ultrasound accelerate phasic calcium phosphate ceramic-induced bone formation?

Does low-intensity pulsed ultrasound accelerate phasic calcium phosphate ceramic-induced bone formation?

Low‐Intensity Pulsed Ultrasound as a Potential Adjuvant Therapy to Promote Spinal Fusion

LIPUS as a potential strategy for periodontitis treatment: A review of the mechanisms

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