Low-intensity pulsed ultrasound enhances immunomodulation and facilitates osteogenesis of human periodontal ligament stem cells by inhibiting the NF-κB pathway

Lin, Haiyan; Wang, Qing; Quan, Chuntian; Ren, Qingyuan; He, Wenting; Xiao, Hui

doi:10.1007/s10561-022-10010-y

Cited by 10 publications

(4 citation statements)

References 54 publications

(67 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…It has been demonstrated that ALP activity in osteoblasts increases from day 5 after daily US stimulation, which can be an indicative of enhanced osteogenesis [22,41]. Also, hPLFs have been reported to synthetize more ALP up to seven days after US stimulation [42,43]. In our study, the ALP expression was constant throughout the experiment for all conditions, with no statistically significant differences being observed between stimulated cells and the control group over three days of daily stimulation.…”

Section: Discussionsupporting

confidence: 49%

Influence of Ultrasound Stimulation on the Viability, Proliferation and Protein Expression of Osteoblasts and Periodontal Ligament Fibroblasts

Pascoal,

Oliveira,

Monteiro

et al. 2024

Biomedicines

View full text Add to dashboard Cite

Among the adjunctive procedures to accelerate orthodontic tooth movement (OTM), ultrasound (US) is a nonsurgical form of mechanical stimulus that has been explored as an alternative to the currently available treatments. This study aimed to clarify the role of US in OTM by exploring different stimulation parameters and their effects on the biological responses of cells involved in OTM. Human fetal osteoblasts and periodontal ligament fibroblasts cell lines were stimulated with US at 1.0 and 1.5 MHz central frequencies and power densities of 30 and 60 mW/cm2 in continuous mode for 5 and 10 min. Cellular proliferation, metabolic activity and protein expression were analyzed. The US parameters that significantly improved the metabolic activity were 1.0 MHz at 30 mW/cm2 for 5 min and 1.0 MHz at 60 mW/cm2 for 5 and 10 min for osteoblasts; and 1.0 MHz at 30 mW/cm2 for 5 min and 1.5 MHz at 60 mW/cm2 for 5 and 10 min for fibroblasts. By stimulating with these parameters, the expression of alkaline phosphatase was maintained, while osteoprotegerin synthesis was induced after three days of US stimulation. The US stimulation improved the biological activity of both osteoblasts and periodontal ligament fibroblasts, inducing their osteogenic differentiation.

show abstract

Section: Discussionsupporting

confidence: 49%

Influence of Ultrasound Stimulation on the Viability, Proliferation and Protein Expression of Osteoblasts and Periodontal Ligament Fibroblasts

Pascoal,

Oliveira,

Monteiro

et al. 2024

Biomedicines

View full text Add to dashboard Cite

show abstract

“…Therefore, this study limits the direct applicability of the results to adult humans or skeletally mature individuals. Another study identified the role for LIPUS in immunomodulation and osteogenesis in hPDLSCs and showed that LIPUS enhanced the immunomodulation and osteogenic differentiation of hPDLSCs by inhibiting the NF-κB signaling pathway in a dose-dependent manner ( 48 ).…”

Section: Mechanisms Involved In the Biological Effects Of Lipus On St...mentioning

confidence: 99%

Latest progress in low-intensity pulsed ultrasound for studying exosomes derived from stem/progenitor cells

He,

Wang,

Deng

et al. 2023

Front. Endocrinol.

View full text Add to dashboard Cite

Stem cells have self-renewal, replication, and multidirectional differentiation potential, while progenitor cells are undifferentiated, pluripotent or specialized stem cells. Stem/progenitor cells secrete various factors, such as cytokines, exosomes, non-coding RNAs, and proteins, and have a wide range of applications in regenerative medicine. However, therapies based on stem cells and their secreted exosomes present limitations, such as insufficient source materials, mature differentiation, and low transplantation success rates, and methods addressing these problems are urgently required. Ultrasound is gaining increasing attention as an emerging technology. Low-intensity pulsed ultrasound (LIPUS) has mechanical, thermal, and cavitation effects and produces vibrational stimuli that can lead to a series of biochemical changes in organs, tissues, and cells, such as the release of extracellular bodies, cytokines, and other signals. These changes can alter the cellular microenvironment and affect biological behaviors, such as cell differentiation and proliferation. Here, we discuss the effects of LIPUS on the biological functions of stem/progenitor cells, exosomes, and non-coding RNAs, alterations involved in related pathways, various emerging applications, and future perspectives. We review the roles and mechanisms of LIPUS in stem/progenitor cells and exosomes with the aim of providing a deeper understanding of LIPUS and promoting research and development in this field.

show abstract

“…LIPUS could facilitate the osteogenesis and microvascular formation of periodontal ligament stem cells [123,124]. Stimulated by LIPUS, autologous ADSC transplantation with fibrin can lead to superior bone-tendon healing quality in the patella-patellar tendon junctions when compared with LIPUS or ADSCs alone.…”

Section: Bone-ligament or Bone-tendon Repairmentioning

confidence: 99%

Musculoskeletal Biomaterials: Stimulated and Synergized with Low Intensity Pulsed Ultrasound

Jia,

Zhou,

Zhan

2023

JFB

View full text Add to dashboard Cite

Clinical biophysical stimulating strategies, which have significant effects on improving the function of organs or treating diseases by causing the salutary response of body, have shown many advantages, such as non-invasiveness, few side effects, and controllable treatment process. As a critical technique for stimulation, the low intensity pulsed ultrasound (LIPUS) has been explored in regulating osteogenesis, which has presented great promise in bone repair by delivering a combined effect with biomaterials. This review summarizes the musculoskeletal biomaterials that can be synergized with LIPUS for enhanced biomedical application, including bone regeneration, spinal fusion, osteonecrosis/osteolysis, cartilage repair, and nerve regeneration. Different types of biomaterials are categorized for summary and evaluation. In each subtype, the verified biological mechanisms are listed in a table or graphs to prove how LIPUS was effective in improving musculoskeletal tissue regeneration. Meanwhile, the acoustic excitation parameters of LIPUS that were promising to be effective for further musculoskeletal tissue engineering are discussed, as well as their limitations and some perspectives for future research. Overall, coupled with biomimetic scaffolds and platforms, LIPUS may be a powerful therapeutic approach to accelerate musculoskeletal tissue repair and even in other regenerative medicine applications.

show abstract

Low-intensity pulsed ultrasound enhances immunomodulation and facilitates osteogenesis of human periodontal ligament stem cells by inhibiting the NF-κB pathway

Cited by 10 publications

References 54 publications

Influence of Ultrasound Stimulation on the Viability, Proliferation and Protein Expression of Osteoblasts and Periodontal Ligament Fibroblasts

Influence of Ultrasound Stimulation on the Viability, Proliferation and Protein Expression of Osteoblasts and Periodontal Ligament Fibroblasts

Latest progress in low-intensity pulsed ultrasound for studying exosomes derived from stem/progenitor cells

Musculoskeletal Biomaterials: Stimulated and Synergized with Low Intensity Pulsed Ultrasound

Contact Info

Product

Resources

About