Low intensity pulse ultrasound stimulate chondrocytes growth in a 3-D alginate scaffold through improved porosity and permeability

Guo, Gepu; Lu, Lu; Ji, Hongfei; Ma, Yong; Dong, Rui; Tu, Juan; Guo, Xiaojun; Qiu, Yuanyuan; Wu, Junru; Zhang, Dong

doi:10.1016/j.ultras.2014.12.001

Cited by 13 publications

(11 citation statements)

References 33 publications

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“…Previous studies demonstrated that non-thermal US can enhance the diffusion of solutes contained within hydrogels, even causing disruption of hydrogel cross-linking [35] or increases in porosity [59]. In this study, US enhanced in vitro dextran release, which correlated with an increase in fibrin degradation in fibrin scaffolds (Figure 4).…”

Section: Discussionsupporting

confidence: 63%

In vitro and in vivo assessment of controlled release and degradation of acoustically responsive scaffolds

et al. 2016

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Spatiotemporally controlled release of growth factors (GFs) is critical for regenerative processes such as angiogenesis. A common strategy is to encapsulate the GF within hydrogels, with release being controlled via diffusion and/or gel degradation (i.e., hydrolysis and/or proteolysis). However, simple encapsulation strategies do not provide spatial or temporal control of GF delivery, especially non-invasive, on-demand controlled release post implantation. We previously demonstrated that fibrin hydrogels, which are widely used in tissue engineering and GF delivery applications, can be doped with perfluorocarbon emulsion, thus yielding an acoustically responsive scaffold (ARS) that can be modulated with focused ultrasound, specifically via a mechanism termed acoustic droplet vaporization. This study investigates the impact of ARS and ultrasound properties on controlled release of a surrogate payload (i.e., fluorescently-labeled dextran) and fibrin degradation in vitro and in vivo. Ultrasound exposure (2.5 MHz, peak rarefactional pressure: 8 MPa, spatial peak time average intensity: 86.4 mW/cm2), generated up to 7.7 and 21.7-fold increases in dextran release from the ARSs in vitro and in vivo, respectively. Ultrasound also induced morphological changes in the ARS. Surprisingly, up to 2.9-fold greater blood vessel density was observed in ARSs compared to fibrin when implanted subcutaneously, even without delivery of pro-angiogenic GFs. The results demonstrate the potential utility of ARSs in generating controlled release for tissue regeneration.

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Section: Discussionsupporting

confidence: 63%

In vitro and in vivo assessment of controlled release and degradation of acoustically responsive scaffolds

et al. 2016

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“…This study demonstrates that LIUS treatment at 5.0‐MHz (14.0‐kPa and <20 mW cm −2 ) significantly enhances the rate of cell proliferation and the colony‐formation efficiency of hMSCs when compared to non‐stimulated controls. In comparison, previous studies that employ LIUS at 1.5‐MHz to enhance the proliferation of various cell types report inconsistent findings ranging from increase in cell numbers, no change and a negative impact . Perhaps these discrepancies lie in the geometry of the culture chamber, culture environment (2D vs. 3D), US frequency and related intensity employed coupled with the cell type.…”

Section: Discussionmentioning

confidence: 85%

Low‐Intensity Ultrasound Upregulates the Expression of Cyclin‐D1 and Promotes Cellular Proliferation in Human Mesenchymal Stem Cells

Budhiraja

Sahu

Subramanian

2018

Biotechnology Journal

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Human mesenchymal stem cells (hMSCs) hold great potential for cellular based therapeutics and tissue engineering applications and their expansion is an interesting prospect due to their low availability from in vivo sources. Therefore, this study investigated the effect of continuous-wave low-intensity ultrasound (LIUS) at 5.0-MHz and 14.0-kPa (<20 mW cm ) on the proliferative capacity, colony-formation efficiency, genetic stability, and differentiation potential of hMSCs. Additionally, potential signaling pathways involved in LIUS-mediated proliferation of hMSCs are studied. Compared to non-stimulated controls, LIUS-treated hMSCs shows a 1.9-fold greater colony-forming efficiency and 2.5-fold higher rate of cell proliferation, respectively. Differential staining and qRT-PCR analysis for selective chondrogenic, osteogenic, and adipogenic markers further confirmed that the LIUS treatment did not impact the multipotency of hMSCs. LIUS-treated hMSCs expressed normal male karyotype. The synthesis of cyclin-D1, a master regulator of cellular proliferation, is upregulated under LIUS and its enhanced mRNA expression under LIUS is noted to be mediated by the activation of both MAPK/ERK and PI3K/AKT pathways. In conclusion, LIUS promotes proliferation and self-renewal capacity of hMSCs.

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“…From the reviewed articles, eight studies have been assessed the pore size influence and two studies demonstrated the effect of porosity on cell behaviors . Scaffolds have been fabricated using various methods including salt leaching, modified lyophilization, freezing, or crosslinking at various temperatures and various acoustic pressure amplitudes to create different pore sizes and porosities . Zhang et al fabricated the collagen scaffolds with the pore size range from 178 to 435 µm.…”

Section: Resultsmentioning

confidence: 99%

Polymeric scaffolds in tissue engineering: a literature review

et al. 2015

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The tissue engineering scaffold acts as an extracellular matrix that interacts to the cells prior to forming new tissues. The chemical and structural characteristics of scaffolds are major concerns in fabricating of ideal three-dimensional structure for tissue engineering applications. The polymer scaffolds used for tissue engineering should possess proper architecture and mechanical properties in addition to supporting cell adhesion, proliferation, and differentiation. Much research has been done on the topic of polymeric scaffold properties such as surface topographic features (roughness and hydrophilicity) and scaffold microstructures (pore size, porosity, pore interconnectivity, and pore and fiber architectures) that influence the cell-scaffold interactions. In this review, efforts were given to evaluate the effect of both chemical and structural characteristics of scaffolds on cell behaviors such as adhesion, proliferation, migration, and differentiation. This review would provide the fundamental information which would be beneficial for scaffold design in future. © 2015 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater, 105B: 431-459, 2017.

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Low intensity pulse ultrasound stimulate chondrocytes growth in a 3-D alginate scaffold through improved porosity and permeability

Cited by 13 publications

References 33 publications

In vitro and in vivo assessment of controlled release and degradation of acoustically responsive scaffolds

In vitro and in vivo assessment of controlled release and degradation of acoustically responsive scaffolds

Low‐Intensity Ultrasound Upregulates the Expression of Cyclin‐D1 and Promotes Cellular Proliferation in Human Mesenchymal Stem Cells

Polymeric scaffolds in tissue engineering: a literature review

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