Low genetic diversity of Anaplasma marginale in calves in an endemic area for bovine anaplasmosis in the state of São Paulo, Brazil

Silva, Jenevaldo Barbosa da; André, Marcos Rogério; Machado, Rosangela Zacarias

doi:10.1016/j.ttbdis.2015.07.018

Cited by 20 publications

(14 citation statements)

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“…In addition, for the isolates described from South America, the most common tandem repeats in the first repetition are 4, 16, 72, α and τ in Brazil (VIDOTTO et al, 2006;POHL et al, 2013;SILVA et al, 2015a, b) and α, τ and B in Argentina (RUYBAL et al, 2009). Previous studies have associated the presence of the strain α-β 3 -Γ with the occurrence of clinical anaplasmosis in Mexico (ALMAZÁN et al, 2008) and Brazil (SILVA et al, 2015b); however, the present study is the first that associates the occurrence of the τ-10-15 strain with animal mortality. Thus, we believe that the A. marginale strains τ-10-15 and α-β 3 -Γ may be involved in the occurrence of bovine anaplasmosis outbreaks in endemic areas.…”

Section: Discussionmentioning

confidence: 96%

“…In Brazil, significant variations in the MSP1a tandem repeats of strains of A. marginale have been found in the states of Paraná (VIDOTTO et al, 2006), Minas Gerais POHL et al, 2013), Rio de Janeiro (SILVA et al, 2015a) and São Paulo (SILVA et al, 2015b). In MSP1a tandem repeats of strains of A. marginale described in Brazil, the most frequent sequences are 16, α, τ in Paraná (VIDOTTO et al, 2006), 72 and α in Minas Gerais (POHL et al, 2013), 4 and τ in Rio de Janeiro (SILVA et al, 2015a) and, α and β in São Paulo (SILVA et al, 2015b). The present study aimed to understand the genetic diversity of A. marginale during an outbreak of bovine anaplasmosis in rural properties located in the municipalities of Lins, state of São Paulo (southeastern), and Mambaí, state of Goiás (central-western), Brazil.…”

Section: Introductionmentioning

confidence: 99%

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Outbreak of anaplasmosis associated with the presence of different Anaplasma marginale strains in dairy cattle in the states of São Paulo and Goiás, Brazil

Machado

Silva

André

et al. 2015

Rev. Bras. Parasitol. Vet.

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The present study reports the genetic diversity of Anaplasma marginale during anaplasmosis outbreaks in rural properties of the states of Goiás and São Paulo, Brazil. Mortality rates of 3.5% (37/1,050) in calves, 4.7% (45/954) in heifers and 1.1% (25/2,200) in lactating cows were observed in a cattle herd of the municipality of Mambaí, state of Goiás, central-western Brazil. In a cattle herd from the municipality of Lins, state of São Paulo, in southeastern Brazil, none of the animals died, despite presenting clinical signs suggestive of bovine anaplasmosis and exhibiting a drastic decrease in milk production. Thus, blood samples were collected from 100 animals with clinical signs suggestive of bovine anaplasmosis in the municipalities of Mambaí and Lins. Based on the microsatellite structure of the MSP1a of A. marginale, the genotypes E and H were observed in Lins, and the C, D and E genotypes were found in Mambaí. The analysis of the tandem repeat structures of the MSP1a showed nine different strains (τ-10 -15, α-β 2 , α-β 3 -13, α-β 2 192, τ-β-100, α-β 2 -Γ, 193-β-100, 191-13-Γ and 191-13-18) in Lins and two (α-β 3 -Γ and E-F-φ 2 -F 2 ) in Mambaí. Three new tandem repeats of MSP1a (191, 192 and 193) were described. The τ-10-15 and α-β 3 -Γ strains were predominantly associated with the occurrence of clinical anaplasmosis and mortality in calves, heifers and lactating cows.

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Section: Discussionmentioning

confidence: 96%

Section: Introductionmentioning

confidence: 99%

Outbreak of anaplasmosis associated with the presence of different Anaplasma marginale strains in dairy cattle in the states of São Paulo and Goiás, Brazil

Machado

Silva

André

et al. 2015

Rev. Bras. Parasitol. Vet.

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“…The iELISA results indicated a high occurrence for A. marginale in the studied region. In the Brazilian territory, this pathogen occurrence in domestic ruminants estimated by iElisa can range from 16.3% in the semiarid Sergipe to close to 100% in Minas Gerais, Paraíba, Bahia, Paraná, Pará, Rio de Janeiro, São Paulo, and Goiás, being considered an endemic agent in Brazil (Oliveira et al, 2013;Silva et al, 2016). Calves and cows were also exposed to A. marginale, due to the constant presence of the tick Rhipicephalus microplus, considered a biological vector, and hematophagous dipterans parasitizing both.…”

Section: Discussionmentioning

confidence: 99%

“…Losses result from low weight gain, reduced milk yield, abortion, treatment costs and mortality (Kocan et al, 2003). Brazil is endemic for A. marginale, with a molecular prevalence in domestic ruminants varying from 7.5% to close to or equal to 100% (Silva et al, 2016). Bovine babesiosis is a hemoparasitosis caused by the protozoa Babesia bovis and Babesia bigemina, which cause fever, anemia, anorexia, lethargy, hemoglobinuria, and muscle tremors (Singh et al, 2009).…”

Section: Introductionmentioning

confidence: 99%

Serological occurrence for tick-borne agents in beef cattle in the Brazilian Pantanal

Ramos

Mello

Mendes

et al. 2020

Rev. Bras. Parasitol. Vet.

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This study investigated the seropositivity for five different tick-borne agents, namely Anaplasma marginale, Babesia bovis, Babesia bigemina, Coxiella burnetii, Anaplasma phagocytophilum, and Trypanosoma vivax in beef cattle in the Brazilian Pantanal. The serum samples collected from animals (200 cows; 200 calves) were used in indirect enzyme-linked immunosorbent assays (iELISA) to detect IgG antibodies against A. marginale, B. bovis, B. bigemina, and T. vivax, and Indirect Fluorescent Antibody Test (IFAT) for detecting IgG antibodies against C. burnetii and A. phagocytophilum. No correlation was observed between seropositivity for C. burnetii and A. phagocytophilum with other agents whereas moderate correlation was observed for A. marginalexB. bigemina x B. bovis. Cows were more seropositive for T. vivax whereas calves were more seropositive for B. bovis and B. bigemina. The highest number of seropositive animals by a single agent was observed for T. vivax (15.2%). Co-seropositivity for T. vivax + A. marginale was higher in cows (25.5%) and for T. vivax + B. bovis + B. bigemina + A. marginale was higher in calves (57.5%). The high seropositivity correlation for A. marginale x B. bovis x B. bigemina is probably due to the presence of the tick biological vector, Rhipicephalus microplus, in the studied farms. Common transmission pathways, mediated by hematophagous dipterans and fomites, may explain the high co-seropositivity of cows for A. marginale and T. vivax. Low seropositivity to C. burnetii is probably due to the type of breeding system employed (extensive). Seropositivity for A. phagocytophilum in only one animal suggests the occurrence of a cross-serological reaction with another agent of the genus Anaplasma.

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“…Erythrocytes are the major sites of infection in cattle [22], in which A. marginale may be seen near the margins of the infected cell. A. marginale can be transmitted transplacentally from dam to calves [24]. A. marginale was previously distributed only in the Okinawa Prefecture [25] although an outbreak was reported in imported cattle in Tokyo in 1989 [26].…”

Section: Introductionmentioning

confidence: 99%

Anaplasma species of veterinary importance in Japan

Ybañez

Inokuma

2016

Vet World

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Anaplasma species of the family Anaplasmataceae, order Rickettsiales are tick-borne organisms that can cause disease in animals and humans. In Japan, all recognized species of Anaplasma (except for Anaplasma ovis) and a potentially novel Anaplasma sp. closely related to Anaplasma phagocytophilum have been reported. Most of these detected tick-borne pathogens are believed to be lowly pathogenic in animals in Japan although the zoonotic A. phagocytophilum has recently been reported to cause clinical signs in a dog and in humans. This review documents the studies and reports about Anaplasma spp. in Japan.

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Low genetic diversity of Anaplasma marginale in calves in an endemic area for bovine anaplasmosis in the state of São Paulo, Brazil

Cited by 20 publications

References 23 publications

Outbreak of anaplasmosis associated with the presence of different Anaplasma marginale strains in dairy cattle in the states of São Paulo and Goiás, Brazil

Outbreak of anaplasmosis associated with the presence of different Anaplasma marginale strains in dairy cattle in the states of São Paulo and Goiás, Brazil

Serological occurrence for tick-borne agents in beef cattle in the Brazilian Pantanal

Anaplasma species of veterinary importance in Japan

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