Low Genetic Diversity Among Garlic (Allium sativum L.) Accessions Detected Using Random Amplified Polymorphic DNA (RAPD)

Abstract

A B S T R A C TGarlic (Allium sativum L.) is a species of vegetative propagation, showing high morphological diversity. Besides, its clones have specific adaptations to different agroclimatic regions. The objective of this study was to determine the genetic diversity of 65 garlic clones collected in Chile and introduced from different countries, by using RAPD (Random Amplified Polymorphic DNA). Fourty random primers of 10 mers generated a total of 398 bands with an 87% of polymorphism. Each primer amplified between two and 20 bands. The size of the fragments obtained fluctuated between 3200 and 369 bp. The results showed that the clones analyzed had a genetic similarity rate of 94%. In addition, 70% of them were clustered in one major group. However, in spite of that situation several clones have different agronomic characteristics.

Inter Simple Sequence Repeat Fingerprints for Assess Genetic Diversity of Tunisian Garlic Populations

“…Accession BJ 17 does not belong to any of those groups, but seems closer to the first one. Neither our results nor the results of Ipek et al (2003) or Paredes et al (2008) noted an association between banding patterns and geographical origins. AFTD analysis reveals grouping that does not reflect morphological variation, or physiological characteristics, particularly the bolting character.…”

“…Depending on the primer, 7 to 21 polymorphic bands were generated with an average of 12, which is comparable to those detected by AFLP in garlic produced from true seeds (12.8-13.9, Ipek et al, 2005) and in populations of leek (11, Smilde et al, 1999). This number of ISSR markers per primer is higher than those generated by RAPD in Brazilian and Chilean garlic (Buso et al, 2008;Paredes et al, 2008). This difference can be due mainly to the method of detection and possibly to the fact that primers are longer, and hence PCR conditions are more stringent (Godwin et al, 1997).…”

“…Tunisia being in the south coast of the Mediterranean basin, great diversity in garlic germplasm is expected. Buso et al (2008) revealed that considerable genetic variation exists among Brazilian garlic cultivars, ranging between 16 and 98% using the RAPD markers, whereas Paredes et al (2008) found little genetic diversity among 69 Chilean garlic clones analyzed by same markers, the percentage of the similarity ranged between 94 and 98%.…”

Lack of genetic diversity of a critically endangered important medicinal plant Chlorophytum borivilianum in Central India revealed by AFLP markers

“…Jatav et al (2011) also observed higher genetic similarity (78 %) using RAPD markers among C. borivilianum accessions collected from different places of Central India. Our results are also in agreements with other vegetatively propagated species which exhibit extremely low or no genetic variations such as Ligusticum chuanxiong (Chen et al 2010), Allium sativum (Paredes et al 2008), Eichhornia crassipes (Li et al 2006), Potamogeton maackianus (Li et al 2004). The presence of a greater morphologic variability for agronomic traits in the collected accessions as compared to the molecular data can be assigned to the different evolutionary and environmental factors.…”

The selection and validation of a marker set for the differentiation of onion cultivars from the Czech Republic

“…Several markers are currently available. RAPD (Random Amplified Polymorphic DNA) is still used for diversity estimates (Paredes et al 2008;Mukherjee et al 2013;Tedeschi et al 2014), as well as AFLP (Amplified Fragment Length Polymorphism), which could be converted into other marker types (Lampasona et al 2003;Filjushin et al 2011;Santos et al 2011;Simo et al 2014). Plant genomes contain polymorphic repetitive sequences that are currently used as DNA markers.…”