The selection and validation of a marker set for the differentiation of onion cultivars from the Czech Republic

Abstract

Mitrová K., Svoboda P., Ovesná J. (2015): The selection and validation of a marker set for the differentiation of onion cultivars from the Czech Republic. Czech J. Genet. Plant Breed., 51: 62-67.The onion (Allium cepa L.) constitutes an important part of the human diet and is of economic importance on the vegetable market. Methods that allow unambiguous differentiation of cultivars and breeding lines are required for market control and the protection of plant breeders' rights. DNA analyses, such as single sequence repeat polymorphism (SSRs), represent such a tool. Based on the evaluation of polymorphism in 21 SSR loci, we established a panel of 15 easy to score SSR markers that differentiated 16 commercial onion cultivars in the Czech Republic. The polymorphism ranged from 2-3 alleles per locus, indicating a low level of diversity among the cultivars. The panel can also be used to evaluate genetic resources.

Antioxidant activity, S-alk(en)yl-l-cysteine sulfoxide and polyphenol content in onion (Allium cepa L.) cultivars are associated with their genetic background

“…Molecular markers, namely SSR, are widely used in plant breeding as they are associated with certain traits (Collard & Mackill 2008). Presented data confirmed that a previously developed SSR marker set (Mitrová et al 2015) could be used not only for cultivar authentication but also as quality markers associated with a certain value of antioxidant capacity. A broader set of onion genotypes cultivated under different environmental conditions or farming practices has to be analysed to validate individual SSR markers as these factors may have impacts upon analysed traits.…”

“…A set of 15 microsatellite markers ( Table 2; Jakše et al 2005) were used to establish the DNA profiles of the analysed onion cultivars. PCR was performed as described by Mitrová et al (2015). The amplification products were separated by capillary electrophoresis in an ABI PRISM 3130 sequencer (Applied Biosystems, Foster City, USA).…”

Molecular characterization of Turkish onion germplasm using SSR markers

“…Other authors reported different allele sizes compared to those described in the original publications. For example, ACM004 gave bands between 201 bp and 212 bp in Czech onion varieties (Mitrová et al 2015); 220 bp and 230 bp in Spanish onion accessions (Mallor et al 2014); and 201 bp and 210 bp in Indian onion accessions (Khar et al 2011). We observed 224 bp and 256 bp bands in Turkish onion accessions using the same marker.…”

“…The ACM238 produced the highest number of bands (14). The EST-SSRs markers showed a higher percentage of amplification than in previous reports (Jakše et al 2005;Khar et al 2011;Mallor et al 2014;Mitrová et al 2015). The bands of ACM315 (290 bp), ACM238 (153 bp) and ACM300 (184 bp, 207 bp, 216 bp) were excluded from further evaluation because of being monomorphic across the studied set of 96 accessions.…”

“…Among these marker systems, simple sequence repeats (SSR; Fischer & Bachmann 2000) and random amplified polymorphic DNA (RAPD; Bradeen & Havey 1995), which are polymerase chain reaction (PCR) based markers, are simple, fast, cheap, and easily automated (Jones et al 1997). Co-dominant simple sequence repeat (SSR) markers have been successfully used to detect genetic diversity and relationships in onion germplasms (Mahajan et al 2009;Anandhan et al 2014;Mitrová et al 2015). The characteristics of genomic microsatellites (gSSR) are abundant in doi: 10.17221/162/2015-CJGPB plant genomes, with reproducibility, high level of polymorphism and codominant inheritance (Nicot et al 2004).…”