Low Frequency Electric Field Induced Changes in the Shape and Motility of Amoebas

Friend, A. W.; Finch, E. D.; Schwan, Herman P.

doi:10.1126/science.1111109

Cited by 46 publications

(21 citation statements)

References 24 publications

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“…3b). Much higher field strengths result in stretching and deformation of the cells, budding and vesicle formation, particularly if the field strength of the a-c field exceeds the breakdown voltage of the membrane [20,58]. At the same time, streaming of the cytoplasm and the organelles is observed (unpublished results) very similar to the phenomenon of cyclosis seen in plant cells and slime molds [3,29,66,67].…”

Section: Membrane Contactsupporting

confidence: 51%

Electric field-induced cell-to-cell fusion

1982

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Cell-to-cell fusion has great potential in membrane research, membrane reconstitution and genetic mapping [25,34,49]. Furthermore, both somatic hybridization and genetic engineering offer a way of modifying plant cells and, in turn, of improving crops [49,53]. If lymphocyte cells are fused with a permanent cell line so-called hybridoma cells are produced [32] which are capable of producing monoclonal antibodies of predetermined antigenic specificity. These hybridoma cells (antibody-producing hybrids) can be envisaged as important tools in future clinical diagnosis and therapy as well as in the purification and enrichment of compounds of cell-biological and medical interest [18,65, 72]. However, despite many promising results and considerable efforts, fusion still seems to be something of an art rather than a precise science.Phenomenologically, cell-to-cell fusion is achieved presently in vitro by chemicals or inactivated virus [2,30,31,33,34,48,64]. Fusion can usually only be achieved by using membrane-disrupting agents and procedures and/or unphysiological conditions (e.g., high Ca/+ concentrations, high or low pH values, hypotonic conditions, etc.)The field of chemically and virus-induced fusion has been excellently reviewed by several authors over the last years [17,34,45,47,48]. In view of the bewildering array of data on chemically and virusinduced fusion we are obliged to conclude that we are still far from solving the molecular mechanism underlying the fusion process.Any progress in this field and in the development of novel fusion techniques will have to be evaluated by comparison with the inherent limitations of the current fusion procedures, which are listed below:

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Section: Membrane Contactsupporting

confidence: 51%

Electric field-induced cell-to-cell fusion

1982

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“…He found no observable effects at 100 V/m, but at 300 V/m morphology of the worms was modified and at 500 V/m they were killed. It is approximately these field strengths which were observed by Friend et al [7] to bring about acute changes in the shape and orientation of amoebae.…”

Section: Discussionmentioning

confidence: 80%

60 Hz electric field parameters associated with the perturbation of a eukaryotic cell system

Miller

Carstensen

Robertson

et al. 1980

Radiat Environ Biophys

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Roots of Pisum sativum were exposed for seven days to 60 Hz electric fields ranging from 70--430 V/m in an aqueous medium whose conductivity was approximately 0.07 mho/m. (Corresponding current densities in the exposure medium associated with these field strengths ranged from 0.5--3.0 mA/cm2). Control and exposed roots were grown concomitantly in the same tank whose growth medium was continuously circulated. Temperature in the exposure medium was held at a constant 19 degrees C. All experiments were conducted "double blind." Root growth rates were determined daily. No perturbations in root growth were observed with electric fields of 150 V/m; there was a slight effect at 360 V/m, and a pronounced decrease in growth rate occurred at 430 V/m. Root conductivities are comparable to that of the growth medium. Under conditions in which growth inhibition occurs, it is estimated that induced 60 Hz cell membrane potentials would be of the order of 3--8 mV.

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“…It is defined by the relaxation of the total membrane tension achieved at the end of the pulse, which is the sum of the electrotension s el and the initial tension s 0 , see eqn (4 . This is probably because the reported experiments are related to dilation rather than to shearing the membrane.…”

Section: Dynamics Of Vesicle Deformation In the Absence Of Saltmentioning

confidence: 99%

“…[1][2][3] Studies of cell deformation in alternating electric (AC) fields have been carried out by many groups and effects on the cell shape and motility have been reported. [4][5][6] Strong electric fields (DC pulses) can cause a significant increase in the electric conductivity and permeability of the cell-plasma membrane. This phenomenon, also referred to as electroporation or electropermeabilization, can be used for introducing various molecules, to which the cell membrane is otherwise impermeable.…”

Section: Introductionmentioning

confidence: 99%

Giant vesicles in electric fields

et al. 2007

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This review is dedicated to electric field effects on giant unilamellar vesicles, a cell-size membrane system. We summarize various types of behavior observed when vesicles are subjected either to weak AC fields at various frequency, or to strong DC pulses. Different processes such as electrodeformation, -poration and -fusion of giant vesicles are considered. We describe some recent developments, which allowed us to detect the dynamics of the vesicle response with a resolution below milliseconds for all of these processes. Novel aspects on electric field effects on vesicles in the gel phase are introduced.

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Low Frequency Electric Field Induced Changes in the Shape and Motility of Amoebas

Cited by 46 publications

References 24 publications

Electric field-induced cell-to-cell fusion

Electric field-induced cell-to-cell fusion

60 Hz electric field parameters associated with the perturbation of a eukaryotic cell system

Giant vesicles in electric fields

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