Giant vesicles in electric fields

Dimova, Rumiana; Riske, Karin A.; Aranda, Said; Bezlyepkina, Natalya; Knorr, Roland L.; Lipowsky, Reinhard

doi:10.1039/b703580b

Cited by 217 publications

(254 citation statements)

References 85 publications

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“…122 They are generally used in biophysical studies of membranes and red blood cells dynamics. 123 Suspended in steady shear flow, vesicles have been observed to exhibit different dynamical modes including tank-treading motion where the vesicle deforms into an ellipsoidal particle with a stationary orientation with respect to the flow direction while its membrane is circulating about its interior. 124 It is notable that in case of a rigid ellipsoid, the particle does not maintain a fixed inclination but will start to tumble in an unsteady fashion.…”

Section: Deformability-selective Cell Separationmentioning

confidence: 99%

Hydrodynamic mechanisms of cell and particle trapping in microfluidics

2013

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Focusing and sorting cells and particles utilizing microfluidic phenomena have been flourishing areas of development in recent years. These processes are largely beneficial in biomedical applications and fundamental studies of cell biology as they provide cost-effective and point-of-care miniaturized diagnostic devices and rare cell enrichment techniques. Due to inherent problems of isolation methods based on the biomarkers and antigens, separation approaches exploiting physical characteristics of cells of interest, such as size, deformability, and electric and magnetic properties, have gained currency in many medical assays. Here, we present an overview of the cell/particle sorting techniques by harnessing intrinsic hydrodynamic effects in microchannels. Our emphasis is on the underlying fluid dynamical mechanisms causing cross stream migration of objects in shear and vortical flows. We also highlight the advantages and drawbacks of each method in terms of throughput, separation efficiency, and cell viability. Finally, we discuss the future research areas for extending the scope of hydrodynamic mechanisms and exploring new physical directions for microfluidic applications.

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Section: Deformability-selective Cell Separationmentioning

confidence: 99%

Hydrodynamic mechanisms of cell and particle trapping in microfluidics

2013

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“…Giant unilamellar vesicles (GUVs) with dimensions of living cells and controlled membrane composition constitute ideal model systems to characterize the properties of lipid assemblies [2]. A variety of techniques have been developed to probe vesicle mechanics, including AFM [3], optical and magnetic stretching [4,5], micropipette aspiration [6], tether pulling [7], shear-flow-based deformation [8], fluctuation spectroscopy [9], and electrodeformation [10][11][12]. In each case (except fluctuation spectroscopy), the common strategy is to investigate the deforming response of the membrane via the application of external forcing.…”

mentioning

confidence: 99%

Ellipsoidal Relaxation of Deformed Vesicles

Lira

Riske

et al. 2015

Phys. Rev. Lett.

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“…This approximation is appropriate for salt-based solutions, such as in vesicle electroporation experiments. 36 For biological cells, and in particular in the cytoplasm, the electrolyte solution has a more complex composition involving both small ions 41 and charged macromolecules ͑proteins, DNA, etc.͒. The latter often possess relatively large charge numbers and small diffusivities.…”

Section: B Multi-ion Effectsmentioning

confidence: 99%

The current-voltage relation for electropores with conductivity gradients

Lin

2010

Biomicrofluidics

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In electroporation, an electric field transiently permeabilizes the cell membrane to gain access to the cytoplasm, and to deliver active agents such as DNA, proteins, and drug molecules. Past work suggests that the permeabilization is caused by the formation of aqueous, conducting pores on the lipid membrane, which are also known as electropores. The current-voltage relation across the membrane-bound pores is critical for understanding and predicting electroporation. In this work, we solve the Nernst-Planck equations in a geometry encompassing an isolated electropore to investigate this relation. In particular, we study cases where the intra-and extracellular electrical conductivities differ. We first derive an analytical solution, which is subsequently validated with a direct numerical simulation using a finite volume method. The main result of the current work is a formula for the effective pore resistance as a function of the pore radius, the membrane thickness, and the intra-and extracellular conductivities. This formula can be incorporated into whole-cell or planar-membrane electroporation models for system-level prediction and understanding.

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Giant vesicles in electric fields

Cited by 217 publications

References 85 publications

Hydrodynamic mechanisms of cell and particle trapping in microfluidics

Hydrodynamic mechanisms of cell and particle trapping in microfluidics

Ellipsoidal Relaxation of Deformed Vesicles

The current-voltage relation for electropores with conductivity gradients

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