Low expression of long noncoding RNA CTC‐297N7.9 predicts poor prognosis in patients with hepatocellular carcinoma

Abstract

Background Long noncoding RNAs (lncRNAs) are reported to play important roles in tumorigenesis of various malignant tumors. However, the clinical significance of aberrant lncRNA expression in hepatocellular carcinoma (HCC) is still elusive. Methods Firstly, a differentially expressed lncRNA CTC‐297N7.9 in HCC was detected by analyzing the data from The Cancer Genome Atlas (TCGA). Secondly, the expression level of CTC‐297N7.9 was examined in four HCC cell lines and 60 pairs of HCC tissues by polymerase chain reaction (PCR) assay at our center. Thirdly, receiver operating characteristic (ROC) analysis was performed to evaluate the diagnostic value of CTC‐297N7.9 for HCC. Correlation and survival analysis of HCC patients from the TCGA and our center were also carried out to assess the predictive value of CTC‐297N7.9. Finally, survival prognostic models were established combining lncRNA expression and other clinical parameters. Results The expression of CTC‐297N7.9 was downregulated in HCC cell lines and HCC tissues. ROC curve revealed its significant diagnostic value in HCC. CTC‐297N7.9 expression correlated with serum alpha‐fetal protein (AFP), tumor stage, and tumor differentiation. Survival analysis indicated that overall survival (OS) and disease‐free survival (DFS) are all positively associated with CTC‐297N7.9 expression, especially in patients without viral hepatitis or cirrhosis. Cox regression analysis showed that CTC‐297N7.9 expression level is an independent prognostic factor for both OS and DFS in HCC patients. Based on the model, CTC‐297N7.9 was observed to be negatively correlated to risk score, indicating its role as a protective factor for HCC. Conclusion Our study demonstrated that the low expression of CTC‐297N7.9 is associated with poor prognosis in HCC patients, suggesting its possible role as a potential prognostic marker for HCC.

Diagnostic and prognostic values of upregulated SPC25 in patients with hepatocellular carcinoma

“…However, the 5-year survival rate of patients with HCC remains low due to the high rates of local recurrence and distant metastasis ( Allemani et al, 2015 ). Several studies have demonstrated that various aberrantly expressed genes can be used to determine HCC prognosis ( Chen et al, 2018b ; Feng et al, 2019 ; Yu et al, 2019 ; Zhu et al, 2019 ). For example, low expression of CTC-297N7.9 is associated with poor prognosis in HCC patients, and could be used as an independent prognostic indicator in HCC patients.…”

“…For example, low expression of CTC-297N7.9 is associated with poor prognosis in HCC patients, and could be used as an independent prognostic indicator in HCC patients. However, its diagnostic capability is limited in discriminating HCC tissues from normal tissues with an AUC of 0.74 ( Zhu et al, 2019 ). The knockdown of TMEM16A inhibited HCC cell proliferation, migration and induced cell apoptosis, and upregulation of TMEM16A induced HCC cell growth, migration and reduced cell apoptosis ( Zhang et al, 2020 ).…”

“…A cell-based assay revealed that the SPC25 can be a potential biomarker for Alzheimer’s disease as the expression level of SPC25 was significantly upregulated in the serum samples of patients with mild cognitive impairment ( Zhang et al, 2018 ). In the HCC cells, PRC1 regulates the expression and function of recurrence-associated genes, such as SPC25, KIF11 FANCI, and KIF23 via Wnt signalling ( Zhu et al, 2019 ). However, the correlation between SPC25 expression and HCC for diagnosis and prognosis of HCC has not been previously evaluated.…”

LncRNA NR_136400 Suppresses Cell Proliferation and Invasion by Acting as a ceRNA of TUSC5 That Is Modulated by miR-8081 in Osteosarcoma

“…The role of lncRNAs in the regulation of cellular processes and cancerous progression is gaining attention (Chi et al, 2019;Lv and Huang, 2019). Zhu et al (2019) reported that the long noncoding RNA CTC-297N7.9 was downregulated in hepatocellular carcinoma and predicted poor prognosis in these patients. LncRNA PCAT-1 was reported as a tumor suppressor in ovarian cancer, and silencing PCAT-1 caused suppression of proliferation, migration, and invasion and promotion of apoptosis (Min and Chu, 2019).…”

Down-regulation of Xist and Mir-7a-5p improves LPS-induced myocardial injury

“…Currently, the pathogenesis of sepsis-induced myocardial damage remains unclear, and thus its treatment remains a challenge. Findings from previous studies indicate that various inflammatory and non-coding RNA are involved in sepsis-induced myocardial damage through modulation, and the latter has received increased research attention in recent years 6 , 7 .…”