Low electromagnetic field (50 Hz) induces differentiation on primary human oral keratinocytes (HOK)

Abstract

This work concerns the effect of low frequency electromagnetic fields (ELF) on biochemical properties of human oral keratinocytes (HOK). Cells exposed to a 2 mT, 50 Hz, magnetic field, showed by scanning electron microscopy (SEM) modification in shape and morphology; these modifications were also associated with different actin distribution, revealed by phalloidin fluorescence analysis. Moreover, exposed cells had a smaller clonogenic capacity, and decreased cellular growth. Indirect immunofluorescence with fluorescent antibodies against involucrin and beta-catenin, both differentiation and adhesion markers, revealed an increase in involucrin and beta-catenin expression. The advance in differentiation was confirmed by a decrease of expression of epidermal growth factor (EGF) receptor in exposed cells, supporting the idea that exposure to electromagnetic field carries keratinocytes to higher differentiation level. These observations support the hypothesis that 50 Hz electromagnetic fields may modify cell morphology and interfere in differentiation and cellular adhesion of normal keratinocytes.

Extremely low frequency electromagnetic field exposure promotes differentiation of pituitary corticotrope‐derived AtT20 D16V cells

“…The solenoid construction reported below, has been published elsewhere [Santoro et al, 1997;Lisi et al, 2000Lisi et al, , 2005Manni et al, 2002Manni et al, , 2004. The main body of the solenoid is a cylinder of concrete-asbestos 2 cm thick and has a diameter of 20 cm and a height of 40 cm.…”

A 50 Hz sinusoidal magnetic field does not damage MG-63 three-dimensional tumor spheroids but induces changes in their invasive properties

“…In fact, in this study, it was shown by immunohistochemistry on paraffin sections that magnetic fields could induce changes in E-cadherin expression in an MNU (N-methyl-N-nitrosurea)-induced colon tumor model. The effects of magnetic fields on CAMs were also shown by the increase in b-catenin expression observed in human oral keratinocytes exposed to a 50 Hz/2 mT sinusoidal magnetic field [Manni et al, 2004]. In this work, cell morphology, growth and clonogenic capacity as well as actin distribution and b-catenin expression were altered.…”

Effects of 50 Hz electromagnetic fields on human epidermal stem cells cultured on collagen sponge scaffolds

“…EMF also could aff ect membrane receptors and a variety of cytokines, and lead to the changes in the capability of cell proliferation. It has been showed that EMF can play a role in the number of epidermal instance, EMF may aff ect cellular membrane structure or associated enzymes, or increase the permeability of small molecules materials, particularly calcium ion infl ux, and regulate intracellular calcium concentration (calcium ion can be a second messenger) to result in changes of cell growth factor receptors of keratinocyte cell surface (Manni et al 2004, Ke et al 2008). In addition, EMF may mediate cell proliferation through directly or indirectly aff ecting gene expression such as calbindin 1, uncoupling protein 3 and cytochrome P450 family 3 subfamily member a (Blank andGoodman 1997, Nikolova et al 2005).…”

“…Recent studies have shown that EMF may act on calmodulin-dependent signaling in tissue repair, and that EMF may represent some of the state-of-the-art therapeutic alternatives for wound healing (Pilla et al 2011, Costin et al 2012). It has been confi rmed that low-intensity/frequency EMF has diff erent biological eff ects on the proliferation and diff erentiation of cells such as bone mesenchymal stem cells, pluripotent embryonic stem cells and keratinocytes (Voroteliak et al 2002, Manni et al 2004, Nikolova et al 2005, Schwartz et al 2008, Sun et al 2009, Tsai et al 2009). …”