Effects of 50 Hz electromagnetic fields on human epidermal stem cells cultured on collagen sponge scaffolds

Bai, Wenfang; Zhang, Mingsheng; Huang, Huiyan; Zhu, Haijing; Xu, Weicheng

doi:10.3109/09553002.2012.692496

Cited by 11 publications

(15 citation statements)

References 37 publications

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“…After cross-linkage with 0.01% glutaraldehyde, the sponge was washed repeatedly with phosphate buffered saline (PBS) for 3 days, and then irradiated using 25 kGy 60 Co (Radiation Center of South China Agriculture University, Guangzhou, China). 1C) using the detailed method that was reported in our previous study [Bai et al, 2012]. 1B).…”

Section: Preparation Of Esc and Collagen Sponge Scaffoldsmentioning

confidence: 99%

“…ESC and collagen sponge scaffolds were obtained using techniques from previously published works [Bai et al, 2012;Zhang et al, 2013]. After cross-linkage with 0.01% glutaraldehyde, the sponge was washed repeatedly with phosphate buffered saline (PBS) for 3 days, and then irradiated using 25 kGy 60 Co (Radiation Center of South China Agriculture University, Guangzhou, China).…”

Section: Preparation Of Esc and Collagen Sponge Scaffoldsmentioning

confidence: 99%

“…The collagen sponges were observed under a scanning electron microscope (Philips XL 30 FEG, Amsterdam, the Netherlands) ( Fig. 1C) using the detailed method that was reported in our previous study [Bai et al, 2012].…”

Section: Preparation Of Esc and Collagen Sponge Scaffoldsmentioning

confidence: 99%

“…1A), 1 ml of 0.25% Trypsin and 0.02% Ethylene Diamine Tetraacetic Acid (EDTA) were added to digest the cultured cells into a single cell with a density of 1 Â 10 6 /ml. The ESC phenotype was confirmed by flow cytometry analysis (FCM; Beckman Coulter, Fullerton, CA) with CD49-FITC (fluorescein isothiocyanate) (positive; Sigma, St. Louis, MO) and CD71-FITC (negative; Sigma) [Bai et al, 2012], as well as observing that the immuno-markers b1 integrin and keratin19 were positive [Ahmadian et al, 2006]. ESC were seeded in collagen sponge scaffolds a day before transplantation and stored in an incubator.…”

Section: Preparation Of Esc and Collagen Sponge Scaffoldsmentioning

confidence: 99%

“…Our previous research demonstrated that ESC cultured in vitro could grow well and realize relatively rapid proliferation under EMF exposure (frequency of 50 Hz, magnetic induction of 5 mT), as well as cells that were seeded in three-dimensional type-I collagen sponge scaffolds [Bai et al, 2012;Zhang et al, 2013]. Most researchers believed that physical factors might inhibit or promote the proliferation of stem cells and affect the number of cells.…”

Section: Introductionmentioning

confidence: 99%

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Efficacy of 50 Hz electromagnetic fields on human epidermal stem cell transplantation seeded in collagen sponge scaffolds for wound healing in a murine model

Bai

Zhu

et al. 2017

Bioelectromagnetics

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To explore the possible efficacy of electromagnetic fields (EMF) for skin tissue engineering, effects of EMF exposure on epidermal stem cells (ESC) seeded in collagen sponge scaffolds for wound healing in a murine model were investigated. The wound models of a full-thickness defect established with 36 7 ∼ 8-week-old nude mice were randomly divided into three groups: a control group, an ESC-only group, and an ESC with EMF exposure group (frequency of 50 Hz, magnetic induction of 5 mT, 60 min per day for 20 days). ESC were separated from human foreskin and cultured in vitro, and then transplanted with collagen sponge scaffolds as a delivery vehicle to wounds of the ESC-only group, and ESC with EMF exposure group was exposed to EMF after ESC transplantation. Effects of EMF on morphological changes and expression of β1 integrin in regenerated skins were observed. Wound healing rates and healing times were collected to evaluate the efficacy of repairment. Results showed that human ESC were successfully transplanted to nude mice, which facilitated the formation of intact skin on nude mice. In contrast to other groups, the wound healing of ESC with EMF exposure group was the fastest (P < 0.05), the structure of regenerated skins was more mature, and it contained more continuity in the number of viable cell layers and rich hair follicles' structure. These results suggest that the use of 50 Hz EMF as a non-invasive treatment can accelerate wound healing of ESC transplantation, and restore structural integrity of regenerated skin. Bioelectromagnetics. 38:204-212,2017. © 2017 Wiley Periodicals, Inc.

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Section: Preparation Of Esc and Collagen Sponge Scaffoldsmentioning

confidence: 99%

Section: Preparation Of Esc and Collagen Sponge Scaffoldsmentioning

confidence: 99%

Section: Preparation Of Esc and Collagen Sponge Scaffoldsmentioning

confidence: 99%

Section: Preparation Of Esc and Collagen Sponge Scaffoldsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Efficacy of 50 Hz electromagnetic fields on human epidermal stem cell transplantation seeded in collagen sponge scaffolds for wound healing in a murine model

Bai

Zhu

et al. 2017

Bioelectromagnetics

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Zinc Sulphate Mediates the Stimulation of Cell Proliferation of Rat Adipose Tissue-Derived Mesenchymal Stem Cells Under High Intensity of EMF Exposure

Fathi

Farahzadi

2017

Biol Trace Elem Res

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Unlike the role of mesenchymal stem cells (MSCs) in regenerative medicine, their application in cell therapy can be complicated by factors such as a reduction in the proliferation potential, senescent tendency of MSCs to expand, and an age-dependent decline in their number and functions. It was shown that there is an association between exposure to electromagnetic fields (EMFs) and response to stress, cell proliferation, aging, and cell death. Furthermore, the zinc ion, as an essential trace element, was reported to be involved in the regulation of the growth and cell proliferation. In this report, ratadipose tissue-derived mesenchymal stem cells (rADSCs) were randomly divided into four groups-group I (control without any ZnSO and EMF); group II (ZnSO treatment without EMF exposed); group III (EMF exposed without ZnSO); and group ІV (EMF exposed with ZnSO)-to evaluate whether 0.14 μg/ml Zinc sulfate (ZnSO) could affect cell proliferation of rADSCs under extremely low frequency-electromagnetic field (ELF-EMF). The methyl thiazoltetrazolium (MTT) method was used to determine the cell proliferation of rADSCs following exposure to ELF-EMF in the presence of ZnSO. The immunocytochemistry method as well as flow-cytometry was used to identify the cell surface markers. Next, oil red O, alizarin red, toluidine blue, and cresyl violet staining was done to evaluate the adipogenic, osteogenic, chondrogenic, and neurogenic differentiation of rADSCs as the pluripotent capacity of rADSCs, respectively. The results showed that an exposure to ELF-EMF caused a decrease in the proliferation of rADSCs. However, the ZnSO supplementation significantly increased the cell proliferation of ELF-EMF-exposed rADSCs. In addition, in the presence of 0.14 μg/ml ZnSO, rADSCs appeared to be growing faster than the control group and ZnSO significantly decreased the doubling time of ELF-EMF-exposed rADSCs. It seems that ZnSO would be a good element to induce the cell proliferation of ELF-EMF-exposed rADSCs.

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Fifty-Hertz electromagnetic fields facilitate the induction of rat bone mesenchymal stromal cells to differentiate into functional neurons

Bai

Feng

et al. 2013

Cytotherapy

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Effects of 50 Hz electromagnetic fields on human epidermal stem cells cultured on collagen sponge scaffolds

Abstract: EMF could significantly promote proliferation of ESC, which leads to a promising clinical option for skin tissue engineering.

Cited by 11 publications

References 37 publications

Efficacy of 50 Hz electromagnetic fields on human epidermal stem cell transplantation seeded in collagen sponge scaffolds for wound healing in a murine model

Efficacy of 50 Hz electromagnetic fields on human epidermal stem cell transplantation seeded in collagen sponge scaffolds for wound healing in a murine model

Zinc Sulphate Mediates the Stimulation of Cell Proliferation of Rat Adipose Tissue-Derived Mesenchymal Stem Cells Under High Intensity of EMF Exposure

Fifty-Hertz electromagnetic fields facilitate the induction of rat bone mesenchymal stromal cells to differentiate into functional neurons

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