Low density lipoproteins extracted from hen egg yolk by an easy method: cryoprotective effect on frozen–thawed bull semen

Moussa, M.; Martinet, Virginie; Trimeche, Abdesselem; Tainturier, D.; Anton, Marc

doi:10.1016/s0093-691x(02)00682-9

Cited by 304 publications

(349 citation statements)

References 29 publications

Supporting

Mentioning

283

Contrasting

Unclassified

Order By: Relevance

“…TE was also used as a control in each experimental series. Extender 3: LDL 1 , the extender with 8% LDL dry matter was prepared in our laboratory according to the technique described by Moussa et al 9 (8% is the optimum concentration found in bull semen by Moussa et al 9 ). This extender was supplemented in the same way as the others with glycerol.…”

Section: Preparation Of the Extendersmentioning

confidence: 99%

“…Many other studies have confirmed that the LDL fraction has cryoprotective action. [8][9][10][11] Egg yolk can therefore be replaced with fractions of LDLs in semen extenders for freezing. An advantage of an LDL extender over a standard egg yolk extender is that it has a less complex chemical composition and does not interfere with microscopic observations.…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Effect of low-density lipoproteins, spermatozoa concentration and glycerol on functional and motility parameters of bull spermatozoa during storage at 4 °C

Vera-Munoz¹,

Amirat-Briand²,

Bencharif³

et al. 2010

Asian J Androl

View full text Add to dashboard Cite

An extender has been developed with low-density lipoproteins (LDLs) that eliminates the microbial risks associated with the use of whole egg yolk. The objective of this study was to assess the effects of substituting egg yolk with LDLs for use as an extender in sperm preservation at 4 6C, as well as on spermatozoa motility, plasma membrane and acrosome integrity, at two different concentrations (80310 6 and 240310 6 sperm per ml) for 8 days and to evaluate glycerol toxicity in both extenders. A total of 12 ejaculates were collected from three bulls. Spermatozoa motility was examined using computer-assisted semen analysis. Plasma membrane integrity was determined using the hypo-osmotic swelling test and acrosome integrity with the fluorescein isothiocyanate-Pisum sativum agglutinin test. The semen was subsequently divided into four aliquots and diluted with Tris-egg yolk-glycerol (TEG), Tris-egg yolk without glycerol (TE), LDL with glycerol (LDL 1 ) and LDL without glycerol (LDL 2 ), at 80310 6 and 240310 6 sperm per ml. This study showed that the LDL 1 and LDL 2 extenders were more effective at preserving spermatozoa motility, plasma membrane integrity and acrosome integrity than TEG and TE (P,0.05) during 8 days of incubation. After 3 days of incubation, a toxicity of glycerol was observed in TEG, whereas no significant difference was observed between LDL 1 and LDL 2 . We can therefore conclude that the LDL extender can be used to refrigerate semen at 4 6C instead of TEG and TE at 80310 6 and 240310 6 sperm per ml for elite bulls. This finding can be used to define a policy for the storage of high-quality bull semen.

show abstract

Section: Preparation Of the Extendersmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Effect of low-density lipoproteins, spermatozoa concentration and glycerol on functional and motility parameters of bull spermatozoa during storage at 4 °C

Vera-Munoz¹,

Amirat-Briand²,

Bencharif³

et al. 2010

Asian J Androl

View full text Add to dashboard Cite

show abstract

“…Lipids are crucial for maintaining the structure and function of membranes when exposed to cryopreservation. As previously reported, low-density lipoprotein, such as lecithin and hen egg yolk, is widely used as a cryoprotective agent in freezing semen in order to protect the membrane's phospholipid integrity against cold shocks [32,33]. The possible protective mechanism of lecithin is related to the protective layer of lecithin at the surface of spermatozoa membranes against ice crystals [33].…”

Section: Discussionmentioning

confidence: 95%

“…As previously reported, low-density lipoprotein, such as lecithin and hen egg yolk, is widely used as a cryoprotective agent in freezing semen in order to protect the membrane's phospholipid integrity against cold shocks [32,33]. The possible protective mechanism of lecithin is related to the protective layer of lecithin at the surface of spermatozoa membranes against ice crystals [33]. Furthermore, these extenders could substitute some phospholipids on the membrane, thereby decreasing the phase transition temperature [34].…”

Section: Discussionmentioning

confidence: 95%

Successful cryopreservation of whole sheep ovary by using DMSO-free cryoprotectant

Lan

Zhao

et al. 2015

J Assist Reprod Genet

View full text Add to dashboard Cite

Purpose The study aims to assess the protective effects of dimethyl sulfoxide (DMSO)-free solution based on trehalose on the cryopreservation of a whole sheep ovary and evaluate its use as an efficient cryoprotectant. Method Twenty-one ovaries collected from 6-to 8-month-old non-pregnant female sheep were randomly distributed into three groups, namely, a fresh group, a DMSO-free group, and a DMSO group. The morphology, cell apoptosis (by hematoxylin and eosin (HE) staining and terminal deoxynucleotidyltransferasemediated dUTP nick-end labeling (TUNEL) assay), and mRNA transcript of Bcl-2-associated X protein (BAX) and cold inducible RNA-binding protein (CIRP) (by real-time PCR) of the thawed sheep ovaries and fresh controls were tested to establish a criterion for appraising the results of the cryopreservation.Results (i) The histological assessment indicated that the structure of the DMSO-free ovaries remained largely intact and comparable to those of the fresh control groups; whereas, significant damage was observed in the ovaries of the DMSO group (P<0.05). (ii) The TUNEL assay and mRNA transcript of the BAX assessment showed that the apoptosis parameter in the fresh group was the lowest among all the groups (P<0.05), and the parameter in the DMSO-free group was significantly lower than that in the DMSO group (P<0.05). (iii) The level of the CIRP transcripts increased the most in the DMSO-free group followed by the DMSO group and the fresh control group (P<0.05). Conclusions These results indicate that a DMSO-free cryoprotectant solution, especially a trehalose cryoprotectant, is an efficient cryoprotectant and has a beneficial effect on the cryopreservation of whole sheep ovaries.Capsule These results indicate that a DMSO-free and trehalose-containing cryoprotectant solution has a beneficial effect on the cryopreservation of intact sheep ovaries.Tianqi Du and Lan Chao contributed equally to this work.

show abstract

“…Though the underlying mechanism of how egg yolk helps sperm survive the cryopreservation process is unknown, it is generally believed that the low-density lipoprotein (LDL) is the main component that offers protection. [1][2][3][4] Recent advances in methods for simple extraction of LDL by Moussa et al 5 promoted a series of studies to test the effectiveness of using LDL to replace whole egg yolk for sperm cryopreservation ( Table 1). A general trend of superiority in using 6%-9% LDL instead of 20% egg yolk was demonstrated in dog, bull, boar and sheep, but not in the Iberian red deer ( Table 1).…”

Section: Introductionmentioning

confidence: 99%

The role of low-density lipoprotein (LDL) and high-density lipoprotein (HDL) in comparison with whole egg yolk for sperm cryopreservation in rhesus monkeys

Dong¹,

Rodenburg²,

Hill³

et al. 2011

Asian J Androl

View full text Add to dashboard Cite

Low-density lipoprotein (LDL) extracted from hen egg yolk has recently been considered to be superior to whole egg yolk in sperm cryopreservation of various animal species. Meanwhile, there was a notion that high-density lipoprotein (HDL) in egg yolk may have a negative effect on post-thaw survival. The role of LDL and HDL in sperm cryopreservation of rhesus monkeys has not been explored. The present study evaluates their effect in comparison with egg yolk with or without the addition of permeable cryoprotectant (glycerol) on sperm cryopreservation of rhesus macaques. In addition, various additives intended to change the lipid composition of LDL-sperm membrane complex have also been tested for their effectiveness in preserving post-thaw viability. Our findings indicated that LDL is the main component in egg yolk that is responsible for its protective role for sperm cryopreservation in rhesus monkeys. Regardless of the presence or absence of glycerol, the protective role of LDL is similar to that of egg yolk and we did not observe any superiority in post-thaw survival with LDL when compared to egg yolk. Modifying the lipid composition of LDL-sperm membrane complex with the addition of cholesterol, cholesterol loaded cyclodextrin and phosphatidylcholine also did not yield any improvements in post-thaw survival; while addition of methyl-b-cyclodextrin reduced post-thaw motility. HDL plays a neutral role in sperm cryopreservation of rhesus monkeys. The present study suggests that egg yolk may still hold advantages when compared with LDL as effective components in extenders for sperm cryopreservation in rhesus monkeys.

show abstract

Low density lipoproteins extracted from hen egg yolk by an easy method: cryoprotective effect on frozen–thawed bull semen

Cited by 304 publications

References 29 publications

Effect of low-density lipoproteins, spermatozoa concentration and glycerol on functional and motility parameters of bull spermatozoa during storage at 4 °C

Effect of low-density lipoproteins, spermatozoa concentration and glycerol on functional and motility parameters of bull spermatozoa during storage at 4 °C

Successful cryopreservation of whole sheep ovary by using DMSO-free cryoprotectant

The role of low-density lipoprotein (LDL) and high-density lipoprotein (HDL) in comparison with whole egg yolk for sperm cryopreservation in rhesus monkeys

Contact Info

Product

Resources

About