Low‐density lipoprotein oxidation inhibition by anthocyanins of wild blueberries

Abstract

The use of natural compounds to prevent oxidative damage in biological systems is becoming increasingly popular. Blueberries have been of specific interest because of their high antioxidant capacity. Anthocyanins were extracted from berries of Vaccinium angustifolium Aiton and Vaccinium myrtilloides Michaux at 79 °C in acidified ethanol with phosphoric acid as a solvent using a liquid/solid ratio of 10. Extracted anthocyanins were purified on a C‐18 silica‐packed column and analyzed by HPLC coupled to electrospray ionization mass spectrometry. Of the six common anthocyanidins, five were identified in the extracts, namely delphinidin, cyanidin, peonidin, petunidin, and malvidin, while pelargonidin was not found. The free radical scavenging effects of anthocyanins were evaluated using N,N‐dimethyl‐p‐phenylenediamine method as well as their potential to mitigate in vitro low‐density lipoprotein (LDL) oxidative damage. After 6 h of incubation, the oxidation products formation such as that of conjugated dienes, trienes, and hexanal was inhibited up to 60 %. Based on these data, we concluded that anthocyanins from V. angustifolium and V. myrtilloides of Quebec possess the ability to inhibit in vitro LDL oxidation.

Preface of the 66th Canadian Chemical Engineering Conference: “Sustainability and Prosperity”

“…This special issue section would have not materialized without the efforts of the contributing authors and the reviewers to ensure, despite their busy schedules, that all accepted papers meet the quality standards of The Canadian Journal of Chemical Engineering . The guest editors are also sincerely thankful to the Editor‐in‐Chief of The Canadian Journal of Chemical Engineering , Prof. João Soares, for his support and guidance, and to his editorial staff for their efforts, in welcoming this series of papers on timely topics.…”