Fatigue behavior (R ε = −1) of HIPed and stress relieved Ti6Al4V alloy specimens produced by selective laser melting (SLM) was analyzed and compared resulting that the hot isostatic pressing (HIP) process caused a microstructural transformation decreasing the hardness and monotonic properties leading to cyclic softening that not allowed fatigue strength to increase. A bilinear behavior in the elastic strain-fatigue life curve was observed due to the decrease of the Young's modulus during the cyclic elastoplastic tests, consequence of subgrains formation. The Smith-Watson-Topper and total strain energy density models adjusted by the bilinear behavior showed a good concordance between predicted and experimental fatigue lives in notched samples.