Low cost optical detection of DNA hybridization on biochips

Pieralli, Christian; Attoui, Ch.; Boireau, Wilfrid; Wacogne, Bruno

doi:10.1134/s1054660x09020182

Cited by 3 publications

(1 citation statement)

References 12 publications

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“…The emergence of the COVID-19 pandemic on one hand and the constant need for early and non-invasive cancer diagnosis on the other hand has made the development of new tools for biomolecular detection an urgent priority. The specific detection of nucleic acids is mainly based on PCR technology [1], identification of antibodies [2], electrochemistry [3] or optical detection methods [4].…”

Section: Introduction 1presentation and State-of-the Artmentioning

confidence: 99%

Optofluidic Sensor Based on Polymer Optical Microresonators for the Specific, Sensitive and Fast Detection of Chemical and Biochemical Species

Keriel,

Delezoide,

Chauvin

et al. 2023

Sensors

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The accurate, rapid, and specific detection of DNA strands in solution is becoming increasingly important, especially in biomedical applications such as the trace detection of COVID-19 or cancer diagnosis. In this work we present the design, elaboration and characterization of an optofluidic sensor based on a polymer-based microresonator which shows a quick response time, a low detection limit and good sensitivity. The device is composed of a micro-racetrack waveguide vertically coupled to a bus waveguide and embedded within a microfluidic circuit. The spectral response of the microresonator, in air or immersed in deionised water, shows quality factors up to 72,900 and contrasts up to 0.9. The concentration of DNA strands in water is related to the spectral shift of the microresonator transmission function, as measured at the inflection points of resonance peaks in order to optimize the signal-over-noise ratio. After functionalization by a DNA probe strand on the surface of the microresonator, a specific and real time measurement of the complementary DNA strands in the solution is realized. Additionally, we have inferred the dissociation constant value of the binding equilibrium of the two complementary DNA strands and evidenced a sensitivity of 16.0 pm/µM and a detection limit of 121 nM.

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