Low-Cost and Effective Fabrication of Biocompatible Nanofibers from Silk and Cellulose-Rich Materials

Guzmán-Puyol, Susana; Heredia‐Guerrero, José A.; Ceseracciu, Luca; Hajiali, Hadi; Canale, Claudio; Scarpellini, Alice; Cingolani, R.; Bayer, Ilker S.; Athanassiou, Athanassia; Mele, Elisa

doi:10.1021/acsbiomaterials.5b00500

Cited by 34 publications

(23 citation statements)

References 59 publications

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“…SF can be processed into nanostructured materials such as nanofibers [27] and nanoparticles [28] or other materials formats including microspheres, films, hydrogels and three-dimensional scaffolds for its superior mechanical and physical properties [29]. In addition with large amounts of amino and carboxyl groups in the side chains of SF, biological and chemical modifications can be performed to engraft special function groups on the SF: Imparting unique properties to SF-based materials [30].…”

Section: Introductionmentioning

confidence: 99%

Porous Silk Fibroin Microspheres Sustainably Releasing Bioactive Basic Fibroblast Growth Factor

Wang

Niu

et al. 2018

Materials

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Basic fibroblast growth factor (bFGF) plays a significant role in stimulating cell proliferation. It remains a challenge in the field of biomaterials to develop a carrier with the capacity of continuously releasing bioactive bFGF. In this study, porous bFGF-loaded silk fibroin (SF) microspheres, with inside-out channels, were fabricated by high-voltage electrostatic differentiation, and followed by lyophilization. The embedded bFGF exhibited a slow release mode for over 13 days without suffering burst release. SEM observations showed that incubated L929 cells could fully spread and produce collagen-like fibrous matrix on the surface of SF microspheres. CLSM observations and the results of cell viability assay indicated that bFGF-loaded microspheres could significantly promote cell proliferation during five to nine days of culture, compared to bFGF-unloaded microspheres. This reveals that the bFGF released from SF microspheres retained obvious bioactivity to stimulate cell growth. Such microspheres sustainably releasing bioactive bFGF might be applied to massive cell culture and tissue engineering as a matrix directly, or after being combined with three-dimensional scaffolds.

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Section: Introductionmentioning

confidence: 99%

Porous Silk Fibroin Microspheres Sustainably Releasing Bioactive Basic Fibroblast Growth Factor

Wang

Niu

et al. 2018

Materials

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“… Naturally-derived biopolymer-based structures with potential application as wound healing systems. (a) examples of protein-based biopolymers primary structures—aminoacidic sequence of collagen type I molecules and aminoacidic sequence of silk fibroin molecules: Gly, glycine; Ala, alanine; Pro, proline; Ser, serine; Hyp, hydroxyproline; (b) natural polysaccharide structures—hyaluronic acid, chitosan, and alginate; (c,d) Biocompatible silk/parsley electrospun fibers (average diameter 50 nm) able to grow NIH3T3 fibroblast cells adapted with permission from Guzman-Puyol et al ( 2016 ) Copyright © 2016 American Chemical Society; (e,f) wool keratin sponges, reprinted from Patrucco et al ( 2016 ) Copyright © 2016 with permission from Elsevier; (g) calcium cross-linked alginate beads and (h) film incorporating antiseptic PVPI complex, reprinted from Liakos et al ( 2013 ) Copyright © 2013 with permission from Elsevier; (i,l) mycelia material from P. ostreatus after 20 days of growth on potato-dextrose broth and cellulose, presenting a 3D network of hyphae. Panels (i,l) are adapted from Haneef et al ( 2017 ).…”

Section: Protein-based Biopolymersmentioning

confidence: 99%

Borrowing From Nature: Biopolymers and Biocomposites as Smart Wound Care Materials

Suarato

Bertorelli

Athanassiou

2018

Front. Bioeng. Biotechnol.

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143

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Wound repair is a complex and tightly regulated physiological process, involving the activation of various cell types throughout each subsequent step (homeostasis, inflammation, proliferation, and tissue remodeling). Any impairment within the correct sequence of the healing events could lead to chronic wounds, with potential effects on the patience quality of life, and consequent fallouts on the wound care management. Nature itself can be of inspiration for the development of fully biodegradable materials, presenting enhanced bioactive potentialities, and sustainability. Naturally-derived biopolymers are nowadays considered smart materials. They provide a versatile and tunable platform to design the appropriate extracellular matrix able to support tissue regeneration, while contrasting the onset of adverse events. In the past decades, fabrication of bioactive materials based on natural polymers, either of protein derivation or polysaccharide-based, has been extensively exploited to tackle wound-healing related problematics. However, in today's World the exclusive use of such materials is becoming an urgent challenge, to meet the demand of environmentally sustainable technologies to support our future needs, including applications in the fields of healthcare and wound management. In the following, we will briefly introduce the main physico-chemical and biological properties of some protein-based biopolymers and some naturally-derived polysaccharides. Moreover, we will present some of the recent technological processing and green fabrication approaches of novel composite materials based on these biopolymers, with particular attention on their applications in the skin tissue repair field. Lastly, we will highlight promising future perspectives for the development of a new generation of environmentally-friendly, naturally-derived, smart wound dressings.

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“…In recent time, electrospun fibroin has been extensively investigated for the design of anti-bacterial, anti-inflammatory and anti-oxidant patches [18,22]. To relieve a water-based electrospinning process, silk has been processed either in combination with natural polymers, such as cellulose, sericin [23], gelatin [24], chitosan [25], or mixed with synthetic materials, such as polyethylene oxide [26], polyvinyl alcohol etc. [17] which yielded great results in developing effective wound healing management [2,4].…”

Section: Silk Fibroin At a Glance:-mentioning

confidence: 99%

Silk Fibroin in Effective Wound Dressing: An Overview.

SinghShera¹,

Das²

2019

IJAR

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Low-Cost and Effective Fabrication of Biocompatible Nanofibers from Silk and Cellulose-Rich Materials

Cited by 34 publications

References 59 publications

Porous Silk Fibroin Microspheres Sustainably Releasing Bioactive Basic Fibroblast Growth Factor

Porous Silk Fibroin Microspheres Sustainably Releasing Bioactive Basic Fibroblast Growth Factor

Borrowing From Nature: Biopolymers and Biocomposites as Smart Wound Care Materials

Silk Fibroin in Effective Wound Dressing: An Overview.

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