Porous Silk Fibroin Microspheres Sustainably Releasing Bioactive Basic Fibroblast Growth Factor

Qu, Jing; Wang, Lu; Niu, Longxing; Lin, Jiaming; Huang, Qian; Jiang, Xuefeng; Li, Mingzhong

doi:10.3390/ma11081280

Cited by 20 publications

(20 citation statements)

References 41 publications

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“…The large surface area of SFMPs offered adequate space for cells to adhere, spread, and proliferate. The pores of SFMPs are also conductive to the precipitation of the extracellular matrix and cellular adhesion, growth, and cell proliferation can be supported [ 37 ]. Therefore, the proliferation of EA.hy926 and HeLa cells was improved after being cultured with 0.9 mg/mL SFMPs ( Figure 7 A).…”

Section: Discussionmentioning

confidence: 99%

“…In our previous study, we prepared silk fibroin microparticles by high-voltage electrospray. Normal cell, mouse fibroblast cells L-929 were adhered to the surface of the microspheres, and cell proliferation was promoted [ 16 , 37 ]. However, the interactions between different cell lines and different-sized silk fibroin particles are not clear.…”

Section: Introductionmentioning

confidence: 99%

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The Interactions of Quantum Dot-Labeled Silk Fibroin Micro/Nanoparticles with Cells

et al. 2020

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When silk fibroin particles are used for controlled drug delivery, particle size plays a key role in the location of the carrier on the cells as well as the transport pathway, utilization efficiency, and therapeutic effect of the drugs. In this study, the interactions of different-sized silk fibroin particles and cell lines were investigated. Silk fibroin microparticles with dry size of 1.9 ± 0.4 μm (2.7 ± 0.3 μm in wet state) and silk fibroin nanoparticles with dry size of 51.5 ± 11.0 nm (174.8 ± 12.5 nm in wet state) were prepared by salting-out method and high-voltage electrospray method, respectively. CdSe/ZnS quantum dots were coupled to the surface of the micro/nanoparticles. Photostability observations indicated that the fluorescence stability of the quantum dots was much higher than that of fluorescein isothiocyanate. In vitro, microparticles and nanoparticles were co-cultured with human umbilical vein endothelial cells EA.hy 926 and cervical cancer cells HeLa, respectively. The fluorescence test and cell viability showed that the EA.hy926 cells tended to be adhered to the microparticle surfaces and the cell proliferation was significantly promoted, while the nanoparticles were more likely to be internalized in HeLa cells and the cell proliferation was notably inhibited. Our findings might provide useful information concerning effective drug delivery that microparticles may be preferred if the drugs need to be delivered to normal cell surface, while nanoparticles may be preferred if the drugs need to be transmitted in tumor cells.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

The Interactions of Quantum Dot-Labeled Silk Fibroin Micro/Nanoparticles with Cells

et al. 2020

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“…The release profiles showed that the PHMB was burst released in the first 12 h and followed by steady release for the next period from the SF sponges for over 20 days ( Figure 5). The loaded PHMB experienced an initial burst release because of the weak physical forces between SF and PHMB, such as Vander Waals' force, hydrogen bonding, and electrostatic adsorption, which were easily broken [38]. The reason for subsequent slow release could be considered to be that the PHMB needed to surmount not only the weak physical forces between SF and PHMB, but also the diffusion barriers created by surrounding SF networks.…”

Section: Discussionmentioning

confidence: 99%

Porous Poly(Hexamethylene Biguanide) Hydrochloride Loaded Silk Fibroin Sponges with Antibacterial Function

et al. 2020

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In order to endue silk fibroin (SF) sponges with antibacterial function, positively charged poly(hexamethylene biguanide) hydrochloride (PHMB) was incorporated in SF through electrostatic interaction and by freeze-drying technique. The influence of PHMB on the structure and antibacterial activities of SF sponges was investigated. The zeta potential of SF was increased significantly when PHMB was incorporated in SF. The pores with size from 80 to 300 µm and the microscale holes in the pore walls within PHMB-loaded SF sponges provided the channels of PHMB release. The PHMB loaded in the porous sponges showed continuous and slow release for up to 20 days. Effective growth inhibition of both Escherichia coli and Staphylococcus aureus was achieved when the mass ratio of PHMB/SF was higher than 2/100. These results suggest that the porous PHMB/SF sponges have the potential to be used as a novel wound dressing for open skin wounds.

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“…A higher initial loading with respect to the microspheres compared to the previous report (MCP-1, 1200 ng/mg; VEGF, 1200 ng/mg) was operated in this study, thus the release of both growth factors could remain for 4 weeks at least. While the growth factor loaded microspheres were composited into the hydrogel, the release of the growth factor was a little delayed from composite hydrogel compared to the bare microspheres [23,32]. However, the release profile of growth factor was mainly controlled by the degradability rate of the microspheres, thus different delivery systems could be facilely constructed by designing the proper formulation of microspheres co-loaded with VEGF and MCP-1, VEGF loaded microsphere, and MCP-1 loaded microsphere in hydrogels (Figure 1).…”

Section: Discussionmentioning

confidence: 99%

Composite Hydrogels with the Simultaneous Release of VEGF and MCP-1 for Enhancing Angiogenesis for Bone Tissue Engineering Applications

Chang¹,

Sun

Huo

et al. 2018

Applied Sciences

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Rapid new microvascular network induction was critical for bone regeneration, which required the spatiotemporal delivery of growth factors and transplantation of endothelial cells. In this study, the linear poly(d,l-lactic-co-glycolic acid)-b-methoxy poly(ethylene glycol) (PLGA-mPEG) block copolymer microspheres were prepared for simultaneously delivering vascular endothelial growth factor (VEGF) and monocyte chemotactic protein-1 (MCP-1). Then, vascular endothelial cells (VECs) with growth factor loaded microspheres were composited into a star-shaped PLGA-mPEG block copolymer solution. After this, composite hydrogel (microspheres ratio: 5 wt%) was formed by increasing the temperature to 37 °C. The release profiles of VEGF and MCP-1 from composite hydrogels in 30 days were investigated to confirm the different simultaneous delivery systems. The VECs exhibited a good proliferation in the composite hydrogels, which proved that the composite hydrogels had a good cytocompatibility. Furthermore, in vivo animal experiments showed that the vessel density and the mean vessel diameters increased over weeks after the composite hydrogels were implanted into the necrosis site of the rabbit femoral head. The above results suggested that the VECs-laden hydrogel composited with the dual-growth factor simultaneous release system has the potential to enhance angiogenesis in bone tissue engineering.

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Porous Silk Fibroin Microspheres Sustainably Releasing Bioactive Basic Fibroblast Growth Factor

Cited by 20 publications

References 41 publications

The Interactions of Quantum Dot-Labeled Silk Fibroin Micro/Nanoparticles with Cells

The Interactions of Quantum Dot-Labeled Silk Fibroin Micro/Nanoparticles with Cells

Porous Poly(Hexamethylene Biguanide) Hydrochloride Loaded Silk Fibroin Sponges with Antibacterial Function

Composite Hydrogels with the Simultaneous Release of VEGF and MCP-1 for Enhancing Angiogenesis for Bone Tissue Engineering Applications

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