Low concentrations of BAP and high rate of subcultures improve the establishment and multiplication of somatic embryos in date palm suspension cultures by limiting oxidative browning associated with high levels of total phenols and peroxidase activities

Abohatem, Mansour; Zouine, Jamila; Hadrami, I. El

doi:10.1016/j.scienta.2011.06.045

Cited by 47 publications

(28 citation statements)

References 28 publications

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“…Extraction and determination of POD activity refer to related articles with some modifications (Abohatem et al, 2011). Under ice bath conditions, the extraction buffer (5.0 mL; 0.2 mol · L -1 ) was added to 5.0 g of fresh chopped lotus root for homogenization (the extraction buffer contained 1 mM polyethyl-ene glycol-6000, 4% polyvinyl pyrrolidone, and 1% Triton X-100).…”

Section: Physicochemical Parametersmentioning

confidence: 99%

Effects of Vacuum Packaging on Enzymatic Browning and Ethylene Response Factor (ERF) Gene Expression of Fresh-cut Lotus Root

Min¹,

Liu²,

Xie³

et al. 2019

horts

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Ethylene response factor (ERF) genes have been involved in responses to biotic and abiotic stress, including hypoxia and anaerobic stress. Vacuum packaging (a typical anaerobic stress) is an effective storage method used to delay browning of fresh-cut lotus root (Nelumbo nucifera). In model plants, ERF genes have been identified as responsive to hypoxia. Whether ERF is associated with browning of vacuum-packaged lotus root has not been studied. The effects of vacuum packaging on browning, phenolic content, the enzyme activity of phenylalanine ammonia lyase (PAL), polyphenol oxidase (PPO), and peroxidase (POD), and PPO, PAL, POD, and ERF genes expression in fresh-cut lotus root were studied. Downregulation of NnPAL1, NnPPOA, and NnPOD2/3 attributable to vacuum packaging coincided with increased related enzyme activities and the degree of browning of fresh-cut lotus root. The expression patterns of NnERF4/5 were consistent with the changes in NnPAL1, NnPPOA, and NnPOD2/3 gene expression. It has been proposed that NnERF4/5 could have be important regulators of fresh-cut lotus root browning, and that the relationships of NnERF4/5 and NnPAL1, NnPPOA, and NnPOD2/3 should to be studied further.

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Section: Physicochemical Parametersmentioning

confidence: 99%

Effects of Vacuum Packaging on Enzymatic Browning and Ethylene Response Factor (ERF) Gene Expression of Fresh-cut Lotus Root

Min¹,

Liu²,

Xie³

et al. 2019

horts

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“…Browning is induced by oxidation on a plant tissue surface. The oxidation of phenolic compounds produces quinones that are highly reactive and toxic to cultured tissues (Zaid, 1984, Abohatem et al, 2011, Al-Samir et al, 2015, Abass, 2016. The browning of cultured date palm tissues is probably one of the causes of growth retardation.…”

Section: Phytotoxic Effect Of Decontamination Agents On Date Palm Promentioning

confidence: 99%

“…This result suggested that the cultured date palm tissues could adapt to biochemical compound accumulation and antioxidant activity caused by fungicide and antibiotic treatments. Proline content, phenol content, and peroxidase activity are positively correlated with plant responses to a wide range of stressors, such as low temperature, water deficiency, salinity, heavy metal exposure, radiation, and high 2,4-D concentrations (Naidu et al, 1991, Hare et al, 1998, Al-Khateb et al, 2002, Munns, 2005, Sharma and Dietz, 2006, Abohatem et al, 2011, El-Samir et al, 2015. An increase in phenol content and peroxidase activity could be one of the causes of browning severity of procallus treated with Beltanol and chloramphenicol (100 mg/l).…”

Section: The Effect Of Decontamination Agents On Date Palm Procallus mentioning

confidence: 99%

Morphological and biochemical impact of different decontamination agents on date palm (Phoenix dactylifera L.) procallus

Abass¹,

Al-Utbi²,

Al-Samir³

2016

Aust J Crop Sci

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This study was conducted to evaluate the phytotoxic effects of different antibiotics and fungicides on the procallus of date palm (Phoenix dactylifera L.). The fungicides Switch® and Beltanol and the antibiotics chloramphenicol and gentamycin were used in this study. Results confirmed the lethal effects of Switch®, gentamycin, and chloramphenicol (at 100 mg/l in dicamba treatment) on date palm tissues. The treated shoot tips did not develop into procallus, and their growth was completely inhibited. Beltanol treatments significantly decreased the biochemical parameters of the procallus treated with 2, 4-D and dicamba. In the Beltanol-treated tissues, the initial period of callus induction was prolonged from 63.33 and 69.66 days to 97.66 and 117.66 days. The callus induction percentage of the plants treated with the decontamination agents was also decreased. The fresh and dry weights of the procallus and the browning percentage and intensity of the cultured tissues in Beltanol and chloramphenicol treatments (100 mg/l) increased compared with those in the control treatments. Total carbohydrates, free proline content, total phenolic compounds, free amino acid, total soluble protein, and peroxidase activity were significantly affected by Beltanol and chloramphenicol (100 mg/l). By contrast, low chloramphenicol concentrations resulted in acceptable levels of biochemical components. Therefore, we recommend the use of low chloramphenicol and Beltanol concentrations instead of Switch and gentamycin as decontamination agents to prevent or treat microbial contamination in date palm tissue cultures.

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“…Deglet Nour, these authors reported the production of 200 embryos from 100 mg fresh weight callus in liquid medium after a 1-month cultivation period, while only 10 embryos were recovered from the same amount of callus on a solidified medium [28]. Therefore, in order to enhance somatic embryo maturation, suspension culture of embryogenic calli has been widely used and was reported in many date palm cultivars such as Jihel and Bousthami Noir [29], Nabout Saif [30], Boufeggouss and Bouskri [31] and Degla Beida [22]. Al-Khayri and Al-Bahrany reported that polyethylene glycol (PEG) stimulates the proliferation of embryos while abscisic acid (ABA) inhibits embryo growth [30].…”

Section: Somatic Embryo Growth Maturation and Germinationmentioning

confidence: 99%

“…This phenomenon was encountered during somatic embryogenesis [31] and organogenesis [54]. It is due to the high levels of caffeoylshikimic acids contained in date palm tissues [63] and leads to explant death.…”

Section: Problems Encountered In Date Palm Micropropagation Tissue Brmentioning

confidence: 99%

Micropropagation of Date Palm: A Review

Mazri¹,

Meziani²

2015

Cell Dev Biol

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Somatic embryogenesis and organogenesis are the two pathways of choice for rapid and large-scale propagation of date palm. They have been successfully used for the micropropagation of elite genotypes and have proved their effectiveness for the commercial production of many cultivars. Nevertheless, regeneration through somatic embryogenesis or organogenesis in date palm is still difficult to achieve for recalcitrant genotypes and is often hampered by certain physiological disorders. In the present review, we report the results of a number of studies carried out on date palm micropropagation. It also describes different factors that influence each stage of somatic embryogenesis and organogenesis and the main problems encountered during these two micropropagation processes.

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Low concentrations of BAP and high rate of subcultures improve the establishment and multiplication of somatic embryos in date palm suspension cultures by limiting oxidative browning associated with high levels of total phenols and peroxidase activities

Cited by 47 publications

References 28 publications

Effects of Vacuum Packaging on Enzymatic Browning and Ethylene Response Factor (ERF) Gene Expression of Fresh-cut Lotus Root

Effects of Vacuum Packaging on Enzymatic Browning and Ethylene Response Factor (ERF) Gene Expression of Fresh-cut Lotus Root

Morphological and biochemical impact of different decontamination agents on date palm (Phoenix dactylifera L.) procallus

Micropropagation of Date Palm: A Review

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