Low-concentration exposure to organochlorine pesticides (OCPs) in L6 myotubes and RIN-m5F pancreatic beta cells induces disorders of glucose metabolism

Acute exposure to environmentally relevant levels of DDT alters muscle mitochondrial function in vivo in rats but not in vitro in L6 myotubes: A pilot study

“…In this pilot study, an acute in vivo exposure to 40 μg/kg of DDT in rats significantly decreased muscle mitochondrial function by 45% at the level of complex I without affecting the expression of mitochondrial complexes. Similarly, and consistent with previous studies [36] , [37] , our in vitro study on L6 muscle cells suggests that only supraphysiological levels of DDT and DDE (1000–10,000 nM) altered maximal respiration, glucose uptake, and response to insulin without alterations in ROS detoxification enzymes.…”

“…Such high concentrations of DDT were shown to induce insulin resistance in L6 myotubes by impairing the phosphorylation of insulin signaling pathway molecules [37] . Furthermore, high concentrations of DDT (1 or 5 μM) on L6 myotubes for 24 and 48 h also inhibited glucose uptake, increased the production of ROS, and downregulated GLUT4 [36] . Altogether, the results indicate that very high concentrations of DDT or DDE are required to alter glucose uptake and insulin sensitivity in muscle cells upon acute exposure in vitro, but that environmentally relevant concentrations do not alter muscle glucose metabolism or insulin sensitivity.…”

“…Reduced cellular response to insulin in skeletal muscle is one of the major components of the development of T2D [35] . Besides the two recent studies that have shown supraphysiological levels of DDT altering glucose metabolism in L6 myotubes [36] , [37] , no studies have investigated the effect of acute exposure to physiologically relevant levels of DDT and DDE on skeletal muscle glucose uptake and mitochondrial function. Therefore, this pilot study aimed to evaluate the effects of acute DDT and DDE exposure on muscle mitochondrial function, glycolysis, glucose uptake, and insulin sensitivity.…”

Evaluation of the Antidiabetic Potential of Extracts of Urtica dioica, Apium graveolens, and Zingiber officinale in Mice, Zebrafish, and Pancreatic β-Cell

“…However, when treated with UAZ, a dose-dependent increase in cell viability was observed compared to the high glucose treatment, indicating that UAZ protects RINm5F cells from high glucose-induced cytotoxicity. Hyperglycemia deteriorates pancreatic insulin secretory capacity and peripheral insulin sensitivity [ 40 ]. UAZ was able to protect pancreatic β cells and improve insulin response.…”

A three‐phase solvent extraction system combined with deep eutectic solvent‐based dispersive liquid–liquid microextraction for extraction of some organochlorine pesticides in cocoa samples prior to gas chromatography with electron capture detection

“…These compounds are characteristically ubiquitous, hydrophobic, carcinogenic, and highly resistant to biological, physical, and chemical degradations [3]. The high lipophilic nature and low metabolism rate of OCPs lead to their accumulation in humans, fish, mammals, and birds tissues through contaminated foods [4]. Several studies showed that OCPs have adverse effects on prostate and liver cancers, diabetes, and birth defects [5].…”