Acute exposure to environmentally relevant levels of DDT alters muscle mitochondrial function in vivo in rats but not in vitro in L6 myotubes: A pilot study

Chehadé, Lucia; Khouri, Hannah; Malatier--Ségard, Julie; Caron, Audrey; Mauger, Jean‐François; Chapados, Natalie Ann; Aguer, Céline

doi:10.1016/j.toxrep.2022.03.004

Cited by 10 publications

(5 citation statements)

References 55 publications

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“…As DDT metabolites reached the soleus muscle, a strong oxidative stress impact was observed as shown by SOD, GPx and LPO, as also previously reported by Chehade et al [58]. The significant inhibition of SOD activity leads to a higher accumulation of superoxide anions in the muscle capable of causing rapid oxidative damage.…”

Section: Muscle Impactsupporting

confidence: 80%

“…This molecule can diffuse through cell membranes, causing oxidative damage and promoting immediate cellular effects, such as structural changes in the cell and the recruitment of immune cells [59,61]. Loss of oxidative capacity in skeletal muscle seems to have a strong correlation to muscular mitochondrial dysfunction due to oxidative damage in mitochondrial proteins [58,62,63]. It is noteworthy to remember that SOD follows a bell-shaped pattern when, after a strong induction, it fails to continue the detoxification role and tends to inhibit levels lower than the basal ones.…”

Section: Muscle Impactmentioning

confidence: 99%

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Metabolic Consequences of the Water We Drink: A Study Based on Field Evidence and Animal Model Experimentation

Wolfart

Theodoro

Silva

et al. 2023

Toxics

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The effect of the chronic consumption of water contaminated with residual concentrations of DDT’s metabolites (DDD—dichlorodiphenyldichloroethane and DDE—dichlorodiphenyldichloroethylene) found in the environment were evaluated on the biometric, hematological and antioxidant system parameters of the hepatic, muscular, renal and nervous tissues of Wistar rats. The results showed that the studied concentrations (0.002 mg.L−1 of DDD plus 0.005 mg.L−1 of DDE) could not cause significant changes in the hematological parameters. However, the tissues showed significant alteration in the activity of the antioxidant system represented by the increase in the activity of the enzymes gluthathione S-transferases in the liver, superoxide dismutase in the kidney, gluthathione peroxidase in the brain, and several changes in enzymatic activity in muscle (SOD, GPx and LPO). The enzymes alanine aminotransaminase (ALT) and aspartate aminotransaminase (AST) were also evaluated for the amino acids’ metabolism in the liver, with ALT showing a significant increase in the exposed animals. In the integrative analysis of biomarkers (Permanova and PCOA), the studied concentrations showed possible metabolic changes and damage to cellular structures evidenced by increased oxidative stress and body weight gain among the treated animals. This study highlights the need for further studies on the impact of banned pesticides still present in soils that may induce adverse effects in organisms that may prevail in future generations and the environment.

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Section: Muscle Impactsupporting

confidence: 80%

Section: Muscle Impactmentioning

confidence: 99%

Metabolic Consequences of the Water We Drink: A Study Based on Field Evidence and Animal Model Experimentation

Wolfart

Theodoro

Silva

et al. 2023

Toxics

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“…However, the visual examination of the cultures ( Figure 1 ) did not support a substantial difference in cell number between cultures initially exposed to DMSO and those exposed to PCB126. Another possible explanation for the decrease in ATP levels observed in cells exposed to PCB126 before differentiation could reside in the fact that POPs have been shown to impair mitochondrial oxygen consumption and ATP production, especially in the longer term, in vivo and in vitro [ 54 , 55 , 56 ]. While further investigations are required to explain the observed delayed decrease in ATP levels 14 days following PCB126 exposure, media LDH activity, cellular ADP/ATP ratio and visual examination strongly suggest that the cells’ viability was not compromised.…”

Section: Discussionmentioning

confidence: 99%

Human Preadipocytes Differentiated under Hypoxia following PCB126 Exposure during Proliferation: Effects on Differentiation, Glucose Uptake and Adipokine Profile

El Amine,

Mauger,

Imbeault

2023

Cells

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Persistent organic pollutants (POPs) accumulation and hypoxia are two factors proposed to adversely alter adipose tissue (AT) functions in the context of excess adiposity. Studies have shown that preadipocytes exposure to dioxin and dioxin-like POPs have the greatest deleterious impact on rodent and immortalized human preadipocyte differentiation, but evidence on human preadipocytes is lacking. Additionally, hypoxia is known to strongly interfere with the dioxin-response pathway. Therefore, we tested the effects of pre-differentiation polychlorinated biphenyl (PCB)126 exposure at 10 µM for 3 days and subsequent differentiation under hypoxia on human subcutaneous adipocytes (hSA) differentiation, glucose uptake and expression of selected metabolism- and inflammation-related genes. Pre-differentiation PCB126 exposure lowered the adenosine triphosphate (ATP) content, glucose uptake and leptin expression of mature adipocytes but had limited effects on differentiation under normoxia (21% O2). Under hypoxia (3% O2), preadipocytes ability to differentiate was significantly reduced as reflected by significant decreased lipid accumulation and downregulation of key adipocyte genes such as peroxisome proliferator-activated receptor gamma (PPARγ) and adiponectin. Hypoxia increased glucose uptake and glucose transporter 1 (GLUT1) expression but abolished the adipocytes insulin response and GLUT4 expression. The expression of pro-inflammatory adipokine interleukin-6 (IL-6) was slightly increased by both PCB126 and hypoxia, while IL-8 expression was significantly increased only following the PCB126-hypoxia sequence. These observations suggest that PCB126 does not affect human preadipocyte differentiation, but does affect the subsequent adipocytes population, as reflected by lower ATP levels and absolute glucose uptake. On the other hand, PCB126 and hypoxia exert additive effects on AT inflammation, an important player in the development of chronic diseases such as type 2 diabetes and cardiovascular diseases.

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“…Indeed, POPs are associated with an inhibition of adrenergic-, epinephrine-, and norepinephrine-induced lipolysis pathways [52]. Other studies have reported the alteration of mitochondrial function with high POPs concentrations in vivo [53]. The ability to prevent the thermogenic response of adipocytes with an AhR agonist (i.e., PCB 126) was also observed under in vitro conditions [54].…”

Section: The Link Between Pops Pa Adipogenesis and Lipid Accumulationmentioning

confidence: 94%

Is Physical Activity an Efficient Strategy to Control the Adverse Effects of Persistent Organic Pollutants in the Context of Obesity? A Narrative Review

Serrano,

Le Garf,

Martin

et al. 2024

IJMS

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Obesity affects nearly 660 million adults worldwide and is known for its many comorbidities. Although the phenomenon of obesity is not fully understood, science regularly reveals new determinants of this pathology. Among them, persistent organic pollutants (POPs) have been recently highlighted. Mainly lipophilic, POPs are normally stored in adipose tissue and can lead to adverse metabolic effects when released into the bloodstream. The main objective of this narrative review is to discuss the different pathways by which physical activity may counteract POPs’ adverse effects. The research that we carried out seems to indicate that physical activity could positively influence several pathways negatively influenced by POPs, such as insulin resistance, inflammation, lipid accumulation, adipogenesis, and gut microbiota dysbiosis, that are associated with the development of obesity. This review also indicates how, through the controlled mobilization of POPs, physical activity could be a valuable approach to reduce the concentration of POPs in the bloodstream. These findings suggest that physical activity should be used to counteract the adverse effects of POPs. However, future studies should accurately assess its impact in specific situations such as bariatric surgery, where weight loss promotes POPs’ blood release.

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Acute exposure to environmentally relevant levels of DDT alters muscle mitochondrial function in vivo in rats but not in vitro in L6 myotubes: A pilot study

Cited by 10 publications

References 55 publications

Metabolic Consequences of the Water We Drink: A Study Based on Field Evidence and Animal Model Experimentation

Metabolic Consequences of the Water We Drink: A Study Based on Field Evidence and Animal Model Experimentation

Human Preadipocytes Differentiated under Hypoxia following PCB126 Exposure during Proliferation: Effects on Differentiation, Glucose Uptake and Adipokine Profile

Is Physical Activity an Efficient Strategy to Control the Adverse Effects of Persistent Organic Pollutants in the Context of Obesity? A Narrative Review

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