Low-capacity cation-exchange chromatography of ultraviolet-absorbing urinary basic metabolites using a reversed-phase column coated with hexadecylsulfonate

Yokoyama, Yukio; Horikoshi, Sohei; Takahashi, Tomoko; Sato, Hisakuni

doi:10.1016/s0021-9673(00)00520-3

Cited by 37 publications

(24 citation statements)

References 12 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Note, that although cre is not an amino acid, it was included in the standard amino aciddipeptide mixture tested in the present work since it coexists with them in some natural samples [1,3].…”

Section: Solutesmentioning

confidence: 99%

Direct RP‐HPLC determination of underivatized amino acids with online dual UV absorbance, fluorescence, and multiple electrochemical detection

Agrafiotou

Sotiropoulos

Pappa-Louisi

2009

J of Separation Science

View full text Add to dashboard Cite

Original PaperDirect RP-HPLC determination of underivatized amino acids with online dual UV absorbance, fluorescence, and multiple electrochemical detectionThe combined use of a dual-UV detector, a fluorimetric one and of a multiple electrochemical (EC) detector equipped with a dual electrode, consisting of a conventional size 3 mm diameter glassy carbon electrode (GCE) and of a pair of 30 lm thick carbon microfibers, is proposed for the determination of 15 amino acids, two dipeptides and creatinine. This online coupling of the above detection modes could partially replace amino acid analysis by derivatization methods, since it solves problems concerning the direct detection of selected underivatized amino acids. Additionally, it was proved that the use of multiple-detection allows positive peak identification in a single chromatographic run, yields more information for free amino acids and solves in some cases the problem of chromatographic resolution. In order to optimize the detection conditions of the underivatized amino acids and related compounds by different detectors, their detection characteristics were determined by adequate preliminary experiments. The electro-oxidation characteristics of the underivatized compounds of interest were determined by hydrodynamic voltammetry using a flow cell with a macrodisc GCE and by ex-situ voltammetry using both a GCE of conventional size and a carbon fiber disk microelectrode. Important practical advantages of microfiber and microdisk electrodes with respect to macroelectrodes were demonstrated.Keywords: Dual electrode / Microdisc electrode / Multiple UV-fluorescence-electrochemical detection / Reversed phase liquid chromatography (RPLC) / Underivatized amino acids and related compounds /

show abstract

Section: Solutesmentioning

confidence: 99%

Direct RP‐HPLC determination of underivatized amino acids with online dual UV absorbance, fluorescence, and multiple electrochemical detection

Agrafiotou

Sotiropoulos

Pappa-Louisi

2009

J of Separation Science

View full text Add to dashboard Cite

show abstract

“…Several methods, mainly high-performance liquid chromatographic (HPLC) (reversed-phase or ion-exchange), have been described for the analysis of histidine-containing dipeptides in matrices from different mammalian species. UV detection has been applied to underivatized peptides, 13,14 but on account of the low extinction coefficients, precolumn derivatization with different chromophores and fluorophores or post-column derivatization (by o-phthalaldehyde or ninhydrin) has been used more often. 15 -21 All these methods suffer from several drawbacks: low resolution, instability of derivatives, in some cases a high detection limit, and above all, they require complex and extensive sample processing and are tedious and time consuming.…”

Section: Carnosine (Car) (ˇ-Alanyl-l-histidine) Homocarnosine (Hcar)mentioning

confidence: 99%

Profiling histidine‐containing dipeptides in rat tissues by liquid chromatography/electrospray ionization tandem mass spectrometry

et al. 2004

View full text Add to dashboard Cite

The histidine-containing dipeptides carnosine (CAR) and structurally related anserine (ANS) and homocarnosine (HCAR), widely distributed in vertebrate organisms, have recently been proposed as endogenous quenchers for highly cytotoxic a,b-unsaturated aldehydes generated by peroxidation. A sensitive, selective, specific and rapid liquid chromatographic/electrospray ionization tandem mass spectrometric assay was developed and validated for the simultaneous determination of these peptides in biological matrices in order to establish their plasma/tissue distribution. Samples (plasma or tissue homogenates from male rats) were prepared by protein precipitation with HClO The intra-and inter-day precisions were <10% (≤17.47% at the LOQ) and the intra-and inter-assay accuracies were within ±10% for all concentrations. The mapping profile in rat tissue gave the following results: the highest concentrations of CAR and ANS were found in skeletal muscles (soleus, gastrocnemius, tibialis), followed by the heart, cerebellum and brain (ANS below the LOQ). HCAR was found only in the brain and cerebellum. No histidine-containing dipeptides were detectable in plasma, liver, kidney and lung.

show abstract

“…HPLC with UV or fluorescence detection has been used for determining imidazole dipeptides in tissues [3,4] and urine samples [5], but the separation was sometimes time-consuming. Moreover, sample pretreatment (such as solid-phase extraction) is often necessary prior to analysis.…”

Section: Introductionmentioning

confidence: 99%

Separation and determination of carnosine-related peptides using capillary electrophoresis with laser-induced fluorescence detection

et al. 2005

View full text Add to dashboard Cite

A capillary electrophoresis (CE) method with laser-induced fluorescence (LIF) detection was developed for the separation and detection of carnosine-related peptides (carnosine, anserine, and homocarnosine). A sensitive and fluorogenic regent, 3-(4-carboxybenzoyl) quinoline-2-carboxaldehyde (CBQCA) was selected as a precapillary labeling reagent for imidazole dipeptides to form isoindole derivatives. The optimized molar ratio between CBQCA and peptide was found to be 75:1, and 50 mmol/L borate buffer (pH 9.2) was used for the derivatization in order to achieve good efficiency. Three imidazole dipeptides were baseline-separated within 20 min by using 112 mmol/L sodium borate (pH 10.4-10.8) as running buffer. Concentration detection limits (signal-to-noise ratios) for carnosine, anserine, and homocarnosine were 4.73, 4.37, and 3.94 nmol/L, respectively. This method has been applied to the analysis of human cerebrospinal fluid (CSF) and meat dry powder of pig and sheep. Recoveries were in the range of 82.9-104.8% for homocarnosine in CSF. For carnosine and anserine, the recoveries are 98.3% and 80.2% in meat dry powder of pig and 111.2% and 112.8% in meat dry powder of sheep, respectively.

show abstract

Low-capacity cation-exchange chromatography of ultraviolet-absorbing urinary basic metabolites using a reversed-phase column coated with hexadecylsulfonate

Cited by 37 publications

References 12 publications

Direct RP‐HPLC determination of underivatized amino acids with online dual UV absorbance, fluorescence, and multiple electrochemical detection

Direct RP‐HPLC determination of underivatized amino acids with online dual UV absorbance, fluorescence, and multiple electrochemical detection

Profiling histidine‐containing dipeptides in rat tissues by liquid chromatography/electrospray ionization tandem mass spectrometry

Separation and determination of carnosine-related peptides using capillary electrophoresis with laser-induced fluorescence detection

Contact Info

Product

Resources

About