Separation and determination of carnosine-related peptides using capillary electrophoresis with laser-induced fluorescence detection

Huang, Ying; Duan, Jianping; Chen, Hongqing; Chen, Min

doi:10.1002/elps.200406130

Cited by 39 publications

(24 citation statements)

References 26 publications

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“…PepT2 may also transport homocarnosine (c-aminobutyryl-L-histidine) and although carnosine is absent from human CSF, homocarnosine is present; furthermore, CSF levels of homocarnosine have been reported to decline with age in humans by a factor of 7 or more (Huang et al 2005;Jansen et al 2006). However, the significance of these observations is uncertain.…”

Section: The Blood-brain Barriermentioning

confidence: 99%

Carnosine and cancer: a perspective

Gaunitz

Hipkiss

2012

Amino Acids

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The application of carnosine in medicine has been discussed since several years, but many claims of therapeutic effects have not been substantiated by rigorous experimental examination. In the present perspective, a possible use of carnosine as an anti-neoplastic therapeutic, especially for the treatment of malignant brain tumours such as glioblastoma is discussed. Possible mechanisms by which carnosine may perform its anti-tumourigenic effects are outlined and its expected bioavailability and possible negative and positive side effects are considered. Finally, alternative strategies are examined such as treatment with other dipeptides or b-alanine.

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Section: The Blood-brain Barriermentioning

confidence: 99%

Carnosine and cancer: a perspective

Gaunitz

Hipkiss

2012

Amino Acids

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“…It is interesting to note that treatment of humans with anticonvulsants gabapentin and topiramate raise levels of homocarnosine, a carnosine homologue, in cerebrospinal fluid (CSF) (Petroff et al, 1998 and2006), and that, while carnosine is absent from human CSF, the levels of CSF homocarnosine decrease markedly with age (Jansen et al, 2006;Huang et al, 2005).…”

Section: Carnosine Anti-convulsant Activity and Agingmentioning

confidence: 99%

On the enigma of carnosine’s anti-ageing actions

Hipkiss¹

2009

Experimental Gerontology

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Carnosine ( -alanyl-L-histidine) has described as a forgotten and enigmatic dipeptide. Carnosine's enigma is particularly exemplified by its apparent anti-ageing actions; it suppresses cultured human fibroblast senescence and delays ageing in senescence-accelerated mice and Drosophila, but the mechanisms reponsible remain uncertain. In addition to carnosine's well-documented anti-oxidant, anti-glycating, aldehyde-scavenging and toxic metal-ion chelating properties, its ability to influence the metabolism of altered polypeptides, whose accumulation characterises the senescent phenotype, should also be considered. When added to cultured cells, carnosine was found in a recent study to suppress phosphorylation of the translational initiation factor eIF4E resulting in decreased translation frequency of certain mRNA species. Mutations in the gene coding for eIF4E in nemtodes extend organism lifespan, hence carnosine's anti-ageing effects may be a consequence of decreased error-protein synthesis which in turn lowers formation of protein carbonyls and increases protease availability for degradation of polypeptides altered postsynthetically. Other studies have revealed carnosine-induced upregulation of stress protein expression and nitric oxide synthesis, both of which may stimulate proteasomal elimination of altered proteins. Some anti-convulsants can enhance nematode longevity and suppress the effects of a protein repair defect in mice, and as carnosine exerts anti-convulsant effects in rodents, it is speculated that the dipeptide may participate in the repair of protein isoaspartyl groups. These new observations only add to the enigma of carnosine's real in vivo functions. More experimentation is clearly required. ACCEPTED MANUSCRIPT 3 Carnosine and ageing.The naturally-occurring dipeptide carnosine ( -alanyl-L-histidine) has been described as forgotten and enigmatic (Bauer, 2005): the present paper is an attempt to rectify the former opinion, but, however only adds to the latter view. Amongst carnosine's enigmatic properties are its apparent anti-ageing actions; the dipeptide is one of a very few naturally-occurring compounds which suppresses cell senescence, induces rejuvenating effects Holliday, 1994 and, and protects against telomere shortening (Shao et al., 2004) in cultured human fibroblasts. The dipeptide also extends the life-span of senescence-accelerated mice (Yuneva et al., 1999) and Drosophila (Yuneva et al., 2002). There are many possible mechanisms by which carnosine exerts these anti-ageing actions however, some of which were summarized a decade ago (Hipkiss, 1998). Meanwhile there have been a number of publications suggesting further possible routes by which carnosine could suppress

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“…However, as peptides and AAs mostly show only little or moderate UV absorbance within the wavelength range commonly used for detection, derivatization procedures are required to facilitate their sensitive detection [15]. LIF [16][17][18][19][20] detection and electrochemical detection [21,22] were another two detection techniques that had been commonly used since they possessed higher detection sensitivity than UV detector. However, derivatization procedures are usually required for LIF detection, and electrochemical detector was only used to detect electroactive substances.…”

Section: Introductionmentioning

confidence: 99%

Rapid analysis of peptides and amino acids by CE‐ESI‐MS using chemically modified fused‐silica capillaries

Zhang

et al. 2009

Electrophoresis

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The objective of this study was to prepare a coated capillary to achieve rapid analysis of peptides and amino acids (AAs) by CE-ESI-MS. The coated capillary was prepared by chemical modification of the fused silica (FS) capillary with 2-acrylamido-2-methyl-1-propanesulfonic acid (AMPS) via a silanization reaction followed by an on-column in situ graft polymerization. In preparing capillary coating, AMPS was introduced for the following two purposes: first, to achieve strong and stable EOF in a wide pH range, and second, to obtain good repeatability of separations of peptides and AAs by reducing their adsorption to the FS capillary. The inner wall morphology, the characteristics of EOF, and the performances of coated capillary including separation ability, repeatability, and stability were investigated in detail and evaluated by analyses of four peptides (D-Ala-D-Ala, Gly-Gly-L-Leu, Gly-D-Phe, and L-Leu-D-Leu) and three AAs (glycine, glutamic acid, and phenylalanine), respectively. Under the optimized conditions, the analysis times of four peptides and three AAs in the coated capillary were 5.6 and 6.0 min, respectively, i.e. much shorter than those in the bare-FS capillary, 20 and 26 min, respectively. These coated capillaries were shown to be well suitable for CE coupling with ESI-MS to analyze peptides and AAs thanks to high separation power, short analysis times, good repeatability and high stability.

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Separation and determination of carnosine-related peptides using capillary electrophoresis with laser-induced fluorescence detection

Cited by 39 publications

References 26 publications

Carnosine and cancer: a perspective

Carnosine and cancer: a perspective

On the enigma of carnosine’s anti-ageing actions

Rapid analysis of peptides and amino acids by CE‐ESI‐MS using chemically modified fused‐silica capillaries

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