Low-affinity cation transporter (
            <i>OsLCT1</i>
            ) regulates cadmium transport into rice grains

Uraguchi, Shimpei; Kamiya, Takehiro; Sakamoto, Takuya; Kasai, Koji; Satō, Yutaka; Nagamura, Yoshiaki; Yoshida, Akira; Kyozuka, Junko; Ishikawa, Shinya; Fujiwara, Toru

doi:10.1073/pnas.1116531109

Cited by 419 publications

(276 citation statements)

References 52 publications

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“…Recently, a transporter, OsLCT1, was reported to be involved in cadmium distribution in rice (Uraguchi et al, 2011). Rice low-affinity cation transporter1 (OsLCT1) showed efflux transport activity for several cations, including cadmium (but not zinc), and knockdown of OsLCT1 resulted in decreased cadmium (but not zinc) concentration in the grain (Uraguchi et al, 2011).…”

Section: Oshma2 Is Also Responsible For Cadmium Partitionmentioning

confidence: 99%

“…Rice low-affinity cation transporter1 (OsLCT1) showed efflux transport activity for several cations, including cadmium (but not zinc), and knockdown of OsLCT1 resulted in decreased cadmium (but not zinc) concentration in the grain (Uraguchi et al, 2011). However, in contrast to OsHMA2 expression, which is highly expressed in the nodes throughout the reproductive growth, expression of OsLCT1 in the nodes was only observed at the ripening stage.…”

Section: Oshma2 Is Also Responsible For Cadmium Partitionmentioning

confidence: 99%

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Preferential Delivery of Zinc to Developing Tissues in Rice Is Mediated by P-Type Heavy Metal ATPase OsHMA2

et al. 2013

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Developing tissues such as meristems and reproductive organs require high zinc, but the molecular mechanisms of how zinc taken up by the roots is preferentially delivered to these tissues with low transpiration are unknown. Here, we report that rice (Oryza sativa) heavy metal ATPase2 (OsHMA2), a member of P-type ATPases, is involved in preferential delivery of zinc to the developing tissues in rice. OsHMA2 was mainly expressed in the mature zone of the roots at the vegetative stage, but higher expression was also found in the nodes at the reproductive stage. The expression was unaffected by either zinc deficiency or zinc excess. OsHMA2 was localized at the pericycle of the roots and at the phloem of enlarged and diffuse vascular bundles in the nodes. Heterologous expression of OsHMA2 in yeast (Saccharomyces cerevisiae) showed influx transport activity for zinc as well as cadmium. Two independent Tos17 insertion lines showed decreased zinc concentration in the crown root tips, decreased concentration of zinc and cadmium in the upper nodes and reproductive organs compared with wild-type rice. Furthermore, a short-term labeling experiment with 67 Zn showed that the distribution of zinc to the panicle and uppermost node I was decreased, but that, to the lower nodes, was increased in the two mutants. Taken together, OsHMA2 in the nodes plays an important role in preferential distribution of zinc as well as cadmium through the phloem to the developing tissues.

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Section: Oshma2 Is Also Responsible For Cadmium Partitionmentioning

confidence: 99%

Section: Oshma2 Is Also Responsible For Cadmium Partitionmentioning

confidence: 99%

Preferential Delivery of Zinc to Developing Tissues in Rice Is Mediated by P-Type Heavy Metal ATPase OsHMA2

et al. 2013

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“…Interestingly, administration of Cd increased translocation of both Cd and Zn in lettuce shoots (Zorrig et al 2010). Low-affinity cation transporter OsLCT1 has been found to load cadmium to phloem sap (Uraguchi et al 2011). Phloem transport of non-essential metals was not accounted for in speciation model of Harris et al (2012).…”

Section: Transport Of Non-essential Metalsmentioning

confidence: 99%

Low-molecular weight organic acids and peptides involved in the long-distance transport of trace metals

Kutrowska

Szeląg

2014

Acta Physiol Plant

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Higher plants evolved mechanisms of uptake, distribution and accumulation of trace metals essential for the proper functioning of the organism (e.g., copper, zinc). Non-essential metals (e.g., cadmium, arsenic, lead) can also enter plant cells using the routes dedicated to the essential ones, because of the shared similar chemical and physical properties. Generally, trace elements are very reactive, able to generate reactive oxygen species and to interact or bind various organic ligands composed of C, H, O, N, P or S. Thus, after entering to the cells, metals are transported and sequestered mainly in a complex form, bound with amino acids, organic acids, peptides or specific metal-binding ligands. Considering diverse properties (e.g., pH value, abundancy of ions, redox state) characterizing cells, tissues and phloem or xylem sap, plants use various ligands to form stable complexes in different conditions. This literature review aims to provide a comprehensive overview on the role of low-molecular weight acids and peptides in trace metals translocation.

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“…BY-2 suspension cells was conducted according to the previous report (Uraguchi et al 2011). The cells transformed with pSU42 (35S-OsPT1-sGFP) or pTS100 (35S-sGFP) (Uraguchi et al 2011) were stained with 25 µM FM4-64 just before imaging analysis and the cells were observed by a laser-scanning confocal microscope (FV1000, Olympus, Tokyo, Japan).…”

Section: Subcellular Localization Of Ospt1-gfpmentioning

confidence: 99%

Phosphate deficiency signaling pathway is a target of arsenate and phosphate transporterOsPT1is involved in As accumulation in shoots of rice

Kamiya

Islam

Duan

et al. 2013

Soil Science and Plant Nutrition

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153

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Arsenate [As(V)] is toxic to organisms, and phosphate (Pi) transporter can mediate As(V) uptake due to their similarity in chemical structure. In the rice (Oriza sativa L.) genome, 13 Pi transporter genes (OsPTs) are present. Their response to As(V) and contribution to As(V) accumulation are unknown. We determined absolute mRNA amount of OsPTs in rice seedlings and OsPT1, OsPT2, OsPT4, and OsPT8 were quantified by real-time polymerase chain reaction (PCR). OsPT2, OsPT4, and OsPT8 were highly induced by Pi deficiency, while OsPT1 was not. In accordance with Pi deficiency response, OsPT2, OsPT4, and OsPT8 induction by Pi deficiency were severely suppressed by As(V). Those data suggest that As(V) affects Pi deficiency signaling in rice. To examine the As(V) transport activity of OsPT1 in planta, we obtained T-DNA mutant of OsPT1 (ospt1). The transcript expression level of OsPT1 in ospt1 was reduced by 70% in shoots and 50% in roots compared to those in the wild-type (WT), and arsenic (As) concentrations in shoots were reduced by 60% compared to WT. We further overexpressed OsPT1-GFP in rice. Overexpression lines showed higher As accumulation in shoots compared to wild-type. OsPT1-GFP is localized to plasma membrane. These results indicate that OsPT1 is involved in As(V) uptake from soil or apoplast.

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Low-affinity cation transporter ( OsLCT1 ) regulates cadmium transport into rice grains

Cited by 419 publications

References 52 publications

Preferential Delivery of Zinc to Developing Tissues in Rice Is Mediated by P-Type Heavy Metal ATPase OsHMA2

Preferential Delivery of Zinc to Developing Tissues in Rice Is Mediated by P-Type Heavy Metal ATPase OsHMA2

Low-molecular weight organic acids and peptides involved in the long-distance transport of trace metals

Phosphate deficiency signaling pathway is a target of arsenate and phosphate transporterOsPT1is involved in As accumulation in shoots of rice

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