Preferential Delivery of Zinc to Developing Tissues in Rice Is Mediated by P-Type Heavy Metal ATPase OsHMA2

Yamaji, Naoki; Xia, Jixing; Mitani‐Ueno, Namiki; Yokosho, Kengo; Feng, Jian

doi:10.1104/pp.113.216564

Cited by 346 publications

(291 citation statements)

References 33 publications

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“…Costaining with 49,6-diamidino-2-phenylindole (DAPI) showed that the fluorescence signal of OsHMA5 was located at peripheral region of the cells outside the nuclei. This localization was similar to OsHMA2, a plasma membrane-localized transporter of Zn and Cd (Yamaji et al, 2013), suggesting that OsHMA5 was also localized to the plasma membrane (Fig. 3, D-F).…”

Section: Cellular and Subcellular Localization Of Oshma5supporting

confidence: 53%

“…A number of transporters for xylem loading have been identified. For example, AtHMA2 and AtHMA4 in A. thaliana and OsHMA2 in rice are responsible for xylem loading of Zn as well as Cd (Hussain et al, 2004;Verret et al, 2004;Yamaji et al, 2013). PHO1 in A. thaliana is involved in the transfer of inorganic phosphate from roots to shoots (Poirier et al, 1991;Chiou and Lin, 2011).…”

Section: Transport Activity Of Oshma5 In Yeastmentioning

confidence: 99%

“…Among rice P-type ATPases characterized, OsHMA2 transports Zn and Cd (Satoh-Nagasawa et al, 2012;Takahashi et al, 2012;Yamaji et al, 2013), OsHMA3 is involved in Cd accumulation (Ueno et al, 2010;Miyadate et al, 2011), and OsHMA9 is responsible for the transport of Zn, Cu, Pb, and Cd (Lee et al, 2007). Knockout of OsHMA5 only resulted in altered Cu concentration, but not other metals, including Zn, Fe, and Mn (Fig.…”

Section: Transport Activity Of Oshma5 In Yeastmentioning

confidence: 99%

“…OsHMA2 was recently reported to be involved in the root-shoot translocation of Zn and Cd (Satoh-Nagasawa et al, 2012;Takahashi et al, 2012;Yamaji et al, 2013). Furthermore, OsHMA2 at the node is required for preferential distribution of Zn to young leaves and panicles (Yamaji et al, 2013).…”

mentioning

confidence: 99%

“…Furthermore, OsHMA2 at the node is required for preferential distribution of Zn to young leaves and panicles (Yamaji et al, 2013). OsHMA3 is localized to the tonoplast of the root cells and responsible for the sequestration of Cd into the vacuoles (Ueno et al, 2010;Miyadate et al, 2011).…”

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confidence: 99%

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A Member of the Heavy Metal P-Type ATPase OsHMA5 Is Involved in Xylem Loading of Copper in Rice

et al. 2013

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ORCID ID: 0000-0003-3411-827X (J.F.M.).Heavy metal-transporting P-type ATPase (HMA) has been implicated in the transport of heavy metals in plants. Here, we report the function and role of an uncharacterized member of HMA, OsHMA5 in rice (Oryza sativa). Knockout of OsHMA5 resulted in a decreased copper (Cu) concentration in the shoots but an increased Cu concentration in the roots at the vegetative stage. At the reproductive stage, the concentration of Cu in the brown rice was significantly lower in the mutants than in the wild-type rice; however, there was no difference in the concentrations of iron, manganese, and zinc between two independent mutants and the wild type. The Cu concentration of xylem sap was lower in the mutants than in the wild-type rice. OsHMA5 was mainly expressed in the roots at the vegetative stage but also in nodes, peduncle, rachis, and husk at the reproductive stage. The expression was up-regulated by excess Cu but not by the deficiency of Cu and other metals, including zinc, iron, and manganese, at the vegetative stage. Analysis of the transgenic rice carrying the OsHMA5 promoter fused with green fluorescent protein revealed that it was localized at the root pericycle cells and xylem region of diffuse vascular bundles in node I, vascular tissues of peduncle, rachis, and husk. Furthermore, immunostaining with an antibody against OsHMA5 revealed that it was localized to the plasma membrane. Expression of OsHMA5 in a Cu transport-defective mutant yeast (Saccharomyces cerevisiae) strain restored the growth. Taken together, OsHMA5 is involved in loading Cu to the xylem of the roots and other organs.

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Section: Cellular and Subcellular Localization Of Oshma5supporting

confidence: 53%

Section: Transport Activity Of Oshma5 In Yeastmentioning

confidence: 99%

Section: Transport Activity Of Oshma5 In Yeastmentioning

confidence: 99%

mentioning

confidence: 99%

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confidence: 99%

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A Member of the Heavy Metal P-Type ATPase OsHMA5 Is Involved in Xylem Loading of Copper in Rice

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Pericycle

Dubrovsky

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2023

Encyclopedia of Life Sciences

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Pericycle is a primary tissue of plant roots with meristematic properties and is the site for the initiation of lateral roots and two secondary meristems, the vascular cambium and phellogen (cork cambium). Pericycle pluripotent cell properties are also revealed during cultivation of root explants in shoot regeneration media. In this article, the characteristics of the pericycle related to each of its functions are reviewed. During lateral root development, five important and coordinated events take place in the pericycle: founder cell identity acquisition, cell division competence, asymmetric cell division, new organ growth axis establishment and lateral inhibition. Cellular and molecular bases of these processes as well as transcriptomic insights in pericycle cell identity are discussed. Cell cycle and related genetic control in pericycle development are also addressed. Cellular and molecular bases of participation of pericycle in the formation of lateral meristems, vascular cambium and phellogen (cork cambium), differences among eudicots and monocots in pericycle development, as well as participation of pericycle in loading of mineral nutrients and unloading of organic compounds are also reviewed. Key Concepts The pericycle is a primary tissue with pluripotent properties that participates in lateral root (LR) formation. In eudicots, it also gives rise to two lateral meristems: vascular cambium and cork cambium (phellogen). Pericycle is typical of plant roots and is the most external cell layer (or layers) of the vascular cylinder. In other plant organs, pericycle or pericycle‐like cell layers can also be present depending on taxon. Outside the root apical meristem, in the root differentiation zone of eudicots, the xylem pole pericycle (XPP) maintains its proliferation activity. Due to its proliferation activity, pericycle possesses high regenerative properties involved in regeneration of shoots from roots. The XPP and phloem pole pericycle (PPP) are characterised by distinct transcriptomic profiles related to their adjacent vascular tissues. Pericycle cells committed for LR development in monocots and eudicots retain the common features related to cell cycle, active auxin synthesis, transport and signalling, and responsiveness to nitrates. LR formation starts from pericycle founder cell specification which is auxin dependent and implies drastic developmental changes in genetic programs involved in asymmetric cell division, new organ growth axis establishment and lateral inhibition. The first founder cell specification is followed by the recruitment of neighbouring pericycle cells that leads to the formation of a morphogenetic field of founder cells giving rise to a LR. The transition from primary to secondary growth starts in the pericycle and procambium and leads to the formation of vascular cambium and phellogen which are coordinated processes dependent on auxin and cytokinin. Pericycle maintains high metabolic activity and participates in loading phloem and xylem and in unloading phloem and thus is involved in shoot‐to‐root and root‐to‐shoot long‐distance transport.

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