Loss of housekeeping selenoprotein expression in mouse liver modulates lipoprotein metabolism

Sengupta, Aniruddha; Carlson, Bradley A.; Hoffmann, Victoria; Gladyshev, Vadim N.; Hatfield, Dolph L.

doi:10.1016/j.bbrc.2007.10.189

Cited by 50 publications

(35 citation statements)

References 21 publications

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“…It has been reported that selenium deficiency results in increased levels of apolipoprotein E in rat plasma although the mechanism by which selenium deficiency affects lipoprotein metabolism is poorly understood [35]. Housekeeping selenoproteins, which would have a role in regulating lipoprotein biosynthesis and metabolism, have been involved [36]. The inverse relationship between selenium deficiency and apolipoprotein E concentration in blood plasma is in contradiction with the observations in this study, probably due to the different nature of selenium used by those authors [35,36].…”

Section: Discussioncontrasting

confidence: 53%

“…Housekeeping selenoproteins, which would have a role in regulating lipoprotein biosynthesis and metabolism, have been involved [36]. The inverse relationship between selenium deficiency and apolipoprotein E concentration in blood plasma is in contradiction with the observations in this study, probably due to the different nature of selenium used by those authors [35,36].…”

Section: Discussionmentioning

confidence: 52%

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Dietary supplementation with selenomethylselenocysteine produces a differential proteomic response

Mahn

Toledo

Ruz

2009

The Journal of Nutritional Biochemistry

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Section: Discussioncontrasting

confidence: 53%

Section: Discussionmentioning

confidence: 52%

Dietary supplementation with selenomethylselenocysteine produces a differential proteomic response

Mahn

Toledo

Ruz

2009

The Journal of Nutritional Biochemistry

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“…Transgenic mice with an inability to synthesize oxidative stress-related selenoproteins presented severe liver and white adipose tissue (WAT) necrosis, ultimately leading to hepatic failure and death at an early stage of life. Hepatic cholesterol biosynthesis was upregulated, resulting in increased plasma cholesterol (56). Thus, selenoproteins appear to play a key role in lipid metabolism.…”

Section: Innovationmentioning

confidence: 96%

Diet-Induced Obesity in the Selenocysteine Lyase Knockout Mouse

Seale

Gilman

Hashimoto

et al. 2015

Antioxidants & Redox Signaling

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Aims: Selenocysteine lyase (Scly) mediates selenocysteine decomposition. It was previously demonstrated that, upon adequate caloric intake (12% kcal fat) and selenium deficiency, disruption of Scly in mice leads to development of metabolic syndrome. In this study, we investigate the effect of a high-fat (45% kcal) seleniumadequate diet in Scly knockout (KO) mice on development of metabolic syndrome. Involvement of selenoproteins in energy metabolism after Scly disruption was also examined in vitro in the murine hepatoma cell line, Hepa1-6, following palmitate treatment. Results: Scly KO mice were more susceptible to diet-induced obesity than their wild-type counterparts after feeding a high-fat selenium-adequate diet. Scly KO mice had aggravated hyperinsulinemia, hypercholesterolemia, glucose, and insulin intolerance, but unchanged inflammatory cytokines and expression of most selenoproteins, except increased serum selenoprotein P (Sepp1). Scly KO mice also exhibited enhanced hepatic levels of pyruvate and enzymes involved in the regulation of pyruvate cycling, such as pyruvate carboxylase (Pcx) and pyruvate dehydrogenase (Pdh). However, in vitro silencing of Scly in Hepa1-6 cells led to diminished Sepp1 expression, and concomitant palmitate treatment decreased Pdh expression. Innovation: The role of selenium in lipid metabolism is recognized, but specific selenium-dependent mechanisms leading to obesity are unclear. This study uncovers that Scly has a remarkable effect on obesity and metabolic syndrome development triggered by high-fat exposure, independent of the expression of most selenoproteins. Conclusion: Diet-induced obesity in Scly KO mice is aggravated, with effects on pyruvate levels and consequent activation of energy metabolism independent of selenoprotein levels. Antioxid. Redox Signal. 23, 761-774.

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“…73 Moreover, in mouse knock-out models in which selenoprotein synthesis is compromised, both liver Apo E protein concentration, plasma cholesterol and expression of genes involved in cholesterol biosynthesis, metabolism and transport are altered, demonstrating a role for selenoproteins in the regulation of lipoprotein biosynthesis. 74 High-dose selenium treatment of rats showed an effect on lipid metabolism,…”

mentioning

confidence: 99%