Loss of genetic variation at microsatellite loci in hatchery strains of the Pacific abalone (Haliotis discus hannai)

Li, Qi; Park, Choul-Ji; Endo, Takashi R.; Kijima, Akihiro

doi:10.1016/j.aquaculture.2003.12.018

Cited by 102 publications

(87 citation statements)

References 30 publications

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“…In the present study, the increase in sampling coverage allowed for a better-resolved genetic structure and enabled us to conclude that the genetic subdivision along the Korean coast is weak and that there is no differentiation between the two groups of wild and hatchery populations. Unlike the present study, previous studies involving Pacific abalone from China and Japan revealed the presence of genetic divergence between the wild and hatchery populations Hara and Sekino, 2007;Li et al, 2004Li et al, , 2007. Given the intensity of Pacific abalone aquaculture activities and stock enhancement programs in Korea, the possibility that they may have partially contributed to the population samples cannot be excluded.…”

Section: Discussioncontrasting

confidence: 88%

“…High levels of genetic diversity seem to be common in marine bivalves because of the large population sizes and high nucleotide mutation rates (Zhan et al, 2007). With an equal number of microsatellite markers, a similar high genetic variability in wild and hatchery populations was also reported for the Japanese and Korean Pacific abalone, respectively (Li et al, 2004;An et al, 2011a). Genetic variability is an important attribute of domesticated species, because those with higher levels of variation are the most likely to have high genetic variances for productive traits (Hurt and Phillip, 2004).…”

Section: Discussionsupporting

confidence: 58%

“…Several studies have assessed the development of a variety of microsatellite markers in Pacific abalone (Sekino and Hara, 2001;Li et al, 2002;Sekino et al, 2005;An et al, 2010a,b), and several population genetic studies using microsatellite loci have reported significant differences of genetic diversity between wild and hatchery abalone stocks in Japan and China (Li et al, 2004Hara and Sekino, 2007). In Korea, there are two reports regarding the genetic characterization of new microsatellite markers from Pacific abalone (An et al, 2010a,b) and only one report on the genetic diversity of hatchery stocks, which found high levels of genetic variation and highly significant F ST values among five hatchery-cultured Pacific abalone populations in Korea (An et al, 2011b).…”

Section: Introductionmentioning

confidence: 99%

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Population genetics of the Pacific abalone (Haliotis discus hannai) in Korea inferred from microsatellite marker analysis

Lee²,

Park³

2012

Genet. Mol. Res.

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ABSTRACT. Populations of the Pacific abalone, Haliotis discus hannai, have been severely overexploited over the past few decades in Korea. Information regarding the levels of genetic variability and structure within populations is insufficient for the development of effective strategies for conservation of genetic diversity of this species. To assess the genetic status of this species, we examined variation at six microsatellite loci in 842 individuals of Pacific abalone collected from three hatchery stocks of the main aquaculture areas and eight wild populations, which were two from the East Sea, two from the West Sea and three from the South Sea. High levels of polymorphism at these microsatellite loci were found in both the wild and hatchery populations. The genetic variation in the hatchery stocks [overall number of alleles (N A ) = 24.00; allelic richness (A R ) = 19.71; observed heterozygosity (H O ) = 0.733] was similar to that of the wild (overall N A = 28.13; A R = 22.62; H O = 0.775) populations. Low levels of inbreeding and significant Hardy-Weinberg equilibrium deviations were detected in both the wild and hatchery populations. Significant F ST values were observed for the hatchery stocks and in most cases between the wild and hatchery populations (overall F ST = 0.017, P < 0.01); however, only a Population genetics of Pacific abalone in Korea minor portion of the genetic diversity was distributed between the wild and hatchery populations. These results reflect intensive seedling and stocking practices. This preliminary study showed genetic separation between the eastern and pooled western and southern wild populations in Korea, which was based on the F ST value, phylogenetic tree clustering, PCA and MDS analyses, structure analysis, and AMOVA. This strong biogeographic structure of H. d. hannai in Korea may be considered to be independent management units. This study demonstrates the feasibility of microsatellite analyses for the monitoring of genetic diversity and for revealing the population structure of the wild Pacific abalone. This information will be useful for the proper management and conservation of H. d. hannai in Korea.

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Section: Discussioncontrasting

confidence: 88%

Section: Discussionsupporting

confidence: 58%

Section: Introductionmentioning

confidence: 99%

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Population genetics of the Pacific abalone (Haliotis discus hannai) in Korea inferred from microsatellite marker analysis

Lee²,

Park³

2012

Genet. Mol. Res.

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“…It has a high degree of individual diversity and co-dominance, and is used for evaluation of genetic diversity and phylogenetic analysis in aquatic organisms. (Norris, Bradley & Cunningham 2000;Li, Park, Endo & Kijima 2004;Barroso, Hilsdorf, Moreira, Cabello & Traub-Cseko 2005).…”

Section: Introductionmentioning

confidence: 99%

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Han¹,

Lee²,

Jung³

et al. 2017

GenAqua

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Turbot (Scophthalmus maximus, Turbot) is a European flounder species, characterized by the absence of scales on the epidermis and the presence of small protuberances. Its capacity to be cultured effectively in fish farms at high densities makes it appealing in the aquaculture industry specifically in the UK, France and Spain. In recent years, development of a new strain by managing genetic resources is being intensively studied in Korea. It is a necessity in aquaculture to check the genetic diversity of a certain species in order to propagate better performing generations of its species. Microsatellite refers to 2-4 nucleotide repeats throughout genome with highly diverse repeat numbers, allowing of which to be used as biological marker for population identification and evaluation of genetic diversity. Population genetic analysis was carried out through the basis of 36 alleles which are different among the groups, presented by eleven markers that could be used in differentiating the genetic platforms of farmed turbot populations from Korea, France, and Turkey. The results obtained can be used in developing markers that are beneficial in identifying superior traits which in turn can be helpful in securing genetic diversity. In conclusion, this study identified genetic differences between farmed turbots in France and Turkey against the S. Korea. Based on the results, it can be implied that the genetic pool is capable of being expanded, and thereby maintenance of genetic diversity and improvement of excellent traits would be possible.

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“…Recent studies have demonstrated the strength of microsatellite DNA markers for monitoring changes in genetic variation of farmed stocks, parentage assignment, and fine-scale studies of population structure in marine species (Norris et al 2000;Desvignes et al 2001;Holland 2001;Alarcón et al 2004;Li et al 2004;An et al 2008). Novel microsatellite markers have been characterized in wild black sea bream and have been found to be highly polymorphic (Jeong et al 2003(Jeong et al , 2007.…”

Section: Introductionmentioning

confidence: 99%

Genetic diversity of wild and farmed black sea bream populations in Jeju

Hong

Lee

et al. 2010

Animal Cells and Systems

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Black sea bream, Acanthopagrus schlegelii, is a commercially important fish in Korea. As a preliminary investigation into the effect of hatchery rearing for stock enhancement, we examined genetic diversity between wild and farmed black sea bream populations from Jeju using six microsatellite markers. High levels of polymorphism were observed between the two populations. A total of 87 different alleles were found at the loci, with some alleles being unique. Allelic variability ranged from 8 to 22 in the wild population and from 7 to 17 in the farmed one. Average observed and expected heterozygosities were estimated at 0.87 and 0.88 in the wild sample. The corresponding estimates were 0.83 and 0.86 in the farmed sample. Although a considerable loss of rare alleles was observed in the farmed sample, no statistically significant reductions were found in heterozygosity or allelic diversity in the farmed sample, compared with the wild one. Significant genetic heterogeneity was found between the wild and farmed populations. These results suggest that more intensive breeding practices for stock enhancement may have resulted in a further decrease of genetic diversity. Thus, it is necessary to monitor genetic variation in bloodstock, progeny, and target populations and control inbreeding in a commercial breeding program for conservation. This information may be useful for fisheries management and the aquaculture industry.

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Loss of genetic variation at microsatellite loci in hatchery strains of the Pacific abalone (Haliotis discus hannai)

Cited by 102 publications

References 30 publications

Population genetics of the Pacific abalone (Haliotis discus hannai) in Korea inferred from microsatellite marker analysis

Population genetics of the Pacific abalone (Haliotis discus hannai) in Korea inferred from microsatellite marker analysis

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Genetic diversity of wild and farmed black sea bream populations in Jeju

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