Loss of Adenosine Triphosphate Synthesis Caused by Freezing and Its Relationship to Frost Hardiness Problems

Heber, U.; Santarius, Kurt A.

doi:10.1104/pp.39.5.712

Cited by 165 publications

(64 citation statements)

References 14 publications

(18 reference statements)

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…In general both freezing injury and hardening are accompanied by a decrease in the SH content of proteins, presumably for different reasons. Specific proteins have shown similar relations in sonme cases (6), whereas in others they have not (3). Because of conflicting results, it is of fundamental importance to investigate the individual protein fractions.…”

mentioning

confidence: 99%

Effects of Freezing and Hardening on the Sulfhydryl Groups of Protein Fractions From Cabbage Leaves

Morton

1969

Plant Physiol.

View full text Add to dashboard Cite

show abstract

mentioning

confidence: 99%

Effects of Freezing and Hardening on the Sulfhydryl Groups of Protein Fractions From Cabbage Leaves

Morton

1969

Plant Physiol.

View full text Add to dashboard Cite

show abstract

“…Sucrose There are a number of reports concerning freezing and animal mitochondria (8,(23)(24)(25)33), but relatively little is known about how freezing affects isolated plant mitochondria. Heber and Santarius (18) reported that frozen and thawed spinach mitochondria partially retained phosphorylative and oxidative properties if sucrose was added, but protection was not as great as for photophosphorylation by chloroplasts. The spinach mitochondria were assayed with a-ketoglutarate as substrate, and all P/O ratios were less than 1.…”

mentioning

confidence: 99%

“…Heber's extensive studies (16)(17)(18) reveal that oxidative phosphorylation and photophosphorylation are inhibited in intact leaves damaged by freezing. The ability of isolated spinach chloroplasts and mitochondria to form ATP is damaged by freezing as well as chloroplast permeability and light-dependent proton uptake.…”

mentioning

confidence: 99%

Freezing Damage to Isolated Tomato Fruit Mitochondria as Modified by Cryoprotective Agents and Storage Temperature

1970

View full text Add to dashboard Cite

Isolated tomato (Lycopersicon esculentum var. Kc 146) fruit mitochondria could be stored successfully in the frozen state without a cryoprotective agent if the mitochondria were frozen quickly by immersion in liquid nitrogen and later thawed quickly at 30 C. Criteria of freezing damage were rate of respiration, adenosine diphosphate to oxygen ratio, and respiratory control ratio. Marked reduction in respiration and loss of respiratory control occurred when mitochondria were transferred from liquid nitrogen to -5, -10, or -18 C for 15 minutes prior to thawing at 30 C.Dimethylsulfoxide (5%Do) prevented freezing danmage when mitochondria were incubated at -5 C but did not prevent freezing damage at -10 or -18 C. There are a number of reports concerning freezing and animal mitochondria (8,(23)(24)(25) 33), but relatively little is known about how freezing affects isolated plant mitochondria. Heber and Santarius (18) reported that frozen and thawed spinach mitochondria partially retained phosphorylative and oxidative properties if sucrose was added, but protection was not as great as for photophosphorylation by chloroplasts. The spinach mitochondria were assayed with a-ketoglutarate as substrate, and all P/O ratios were less than 1. Hence, these workers may have observed the effect of freezing on the substrate level phosphorylation only.Our earlier work (5) showed that isolated tomato fruit mitochondria were severely damaged by freezing. Oxygen uptake decreased 89 /0 or more after 24 hr of storage at -18 C or in liquid nitrogen, and the damaged mitochondria lost respiratory control. The cryoprotective agent dimethylsulfoxide prevented freezing damage during storage in liquid nitrogen, and mitochondria were stored a month without decrease in ADP/O or loss of respiratory control.The present study also deals with freezing of isolated tomato mitochondria and is a continuation of the earlier work. Experiments were conducted to learn if DMSO3 was the only effective cryoprotective agent, whether rate of warming was related to a need for a cryoprotective agent, and the time course of freezing damage at several subzero temperatures.The cause of freezing damage is still not completely known (8,17,28), and the mode of action of cryoprotective agents is not clear (3,22,27,31). Isolated tomato mitochondria show promise for studying these phenomena. MATERIALS AND METHODSTomato fruit mitochondria were isolated and assayed with a Clark oxygen electrode as described earlier (5-7). Outer pericarp from mature green tomato fruits (Lycopersicon esculentunm, var.Kc 146) was ground at 0 C in a food mill with a medium (2 g of medium per g of tissue) containing 0.5 M mannitol, 0.05 Ni Na barbital (pH 7.8), 4 mm cysteine, 5 mm EDTA, and 1.5 mg/ml bovine serum albumin fraction V powder. The homogenate was strained through muslin, and mitochondria were collected as the fraction precipitating between 1,500g for 15 min and 15,000g for 15 min. Mitochondria were washed once in 0.5 M mannitol, 5 mm Na barbital, 1.5 mg,'ml bovine serum albumi...

show abstract

“…Freezing injury has been postulated to denature cellularproteins, probably by changes in ionic strength and pH of the cell (Heber and Santarius 1964). Membrane destruction by freezing injury with subsequent release of enzymes and cellular constituents would further create disorganization of biochemical processes and inactivation of active transport systems (Burke et al 1916 Palta andLi 1978).…”

mentioning

confidence: 99%

Effect of Freezing Injury on Certain Chemical and Physical Properties of Flue-Cured Tobacco

Rosa¹,

Elliot²,

Court³

et al. 1984

Can. J. Plant Sci.

View full text Add to dashboard Cite

show abstract

Loss of Adenosine Triphosphate Synthesis Caused by Freezing and Its Relationship to Frost Hardiness Problems

Cited by 165 publications

References 14 publications

Effects of Freezing and Hardening on the Sulfhydryl Groups of Protein Fractions From Cabbage Leaves

Effects of Freezing and Hardening on the Sulfhydryl Groups of Protein Fractions From Cabbage Leaves

Freezing Damage to Isolated Tomato Fruit Mitochondria as Modified by Cryoprotective Agents and Storage Temperature

Effect of Freezing Injury on Certain Chemical and Physical Properties of Flue-Cured Tobacco

Contact Info

Product

Resources

About