Effects of Freezing and Hardening on the Sulfhydryl Groups of Protein Fractions From Cabbage Leaves

Morton, William M.

doi:10.1104/pp.44.2.168

Cited by 17 publications

(8 citation statements)

References 6 publications

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“…The mechanism for this is unclear, but could be the result of freezing on sulfhydryl groups that could potentially impair Pb binding on the ASV. Freezing of tissues is known to decrease sulfhydryls (e.g., glutathione; Morton , Stuiver et al , Wang , Gadea et al ) and subsequent thawing results in a continued reduction of glutathione (Stuiver et al ). Alternatively, freezing samples may result in more complete lysing of red blood cells and increased availability of free Pb for detection by the ASV unit.…”

Section: Discussionmentioning

confidence: 99%

Critically assessing the utility of portable lead analyzers for wildlife conservation

Herring

Eagles‐Smith

Bedrosian³

et al. 2018

Wildl. Soc. Bull.

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Lead (Pb) exposure in wildlife is a widespread management and conservation concern. Quantitative determination of Pb concentrations in wildlife tissues is the foundation for estimating exposure and risk. Development of low-cost, portable instruments has improved access and cost-effectiveness of determining Pb concentrations in blood samples, while also facilitating the ability for wildlife researchers to conduct near real-time Pb testing. However, these instruments, which use anodic stripping voltammetry (ASV) methodology, may produce an analytical bias in wildlife-blood Pb concentrations. Additionally, their simplicity invites use without appropriate quality-assurance-quality-control measures. Together, these factors can reduce data quality and hamper the ability to evaluate it, raising concerns about use of these instruments to inform important conservation issues. We document the extent to which this bias is addressed in the wildlife toxicology literature, develop quantitative approaches for correcting the bias, and provide recommendations to ensure robust data quality when using these instruments. Of the 25 studies we reviewed that referenced ASV use for determining Pb exposure in wildlife, only 32% acknowledged the existence of bias from the instrument. Importantly, another 20% of the studies actually reported ASV and spectroscopic-based results together without acknowledging their lack of equivalence. Using a multispecies data set of avian blood Pb concentrations, we found that ASV-based estimates of paired blood Pb concentrations were 30-38% lower than those from standard spectrometric-based methods. We provide regression equations based on this analysis of 453 blood samples to allow users of ASV instruments to adjust Pb concentrations to spectrometricequivalent values, and propose a series of guidelines to follow when using these instruments to improve data validity. Published 2018. This article is a U.S. Government work and is in the public domain in the USA.KEY WORDS anodic stripping voltammetry, blood lead, graphite-furnace atomic absorption spectrometry, inductively-coupled mass spectrometry, LeadCare 1 , portable lead analyzer.

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Section: Discussionmentioning

confidence: 99%

Critically assessing the utility of portable lead analyzers for wildlife conservation

Herring

Eagles‐Smith

Bedrosian³

et al. 2018

Wildl. Soc. Bull.

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“…The biochemistry of plants adapting to or damaged by near-freezing temperatures is well documented (Biglov, 1964;Kuraishi et aI., 1968;Matsko, 1963;Morton, 1969;Parker, 1962;Siminovitch, 1963;Teltscherova, 1963;Toman and Mitchell, 1968). On the other hand, relatively little study has been given to ultrastructural changes caused by low temperatures.…”

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confidence: 99%

Ultrastructural Changes of Plants Exposed to Low Temperatures

Kimball

Salisbury

1973

American J of Botany

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Effects of low temperatures on cell ultrastructures of three grass species were studied. Secale cereale L., Cynodon dactylon (L.) Pers., and Paspalum notatum Flugge seedlings were given treatments of −5, 0, 10, or 25 C for 3 days and then evaluated for cellular response. Electron micrographs showed chloroplasts to be the most sensitive organelle in all three species, with the temperate Secale plastids responding differently than plastids from the subtropical and tropical species. Rough endoplasmic reticulum increased, but dictyosome numbers decreased in all species as treatment temperatures were lowered to 0 C. Mitochondria expanded slightly at reduced temperatures, but with little difference detected between species. The study indicated that the various cellular components of these species responded differently to reduced temperatures, with the species differing mainly in degree of response.

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“…We explain this by a possible replacement of pMBA by intrinsic biological molecules on the surface of the onion layer. It is known that proteins can contain SH groups, and also other bioactive compounds containing sulfur compounds have been predicted in onions such as quercetin and diallyl sulfides . It is well known that sulfur has a strong affinity to silver.…”

Section: Resultsmentioning

confidence: 99%

Raman spectroscopic probing of plant material using SERS

Palanco

Mogensen

Kneipp

2015

J Raman Spectroscopy

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We report surface-enhanced Raman studies on intact plant material using onion layers as a biological target, and silver nanoaggregates and silver island films as enhancing plasmonic structures. Surface-enhanced Raman scattering (SERS) enhancement allows the detection of strong Raman signatures of chemical constituents of the surface of the onion layer such as cellulose, proteins, and flavonols. Because of long-time incubation, SERS sensors can access the extracellular space in the inner of the layer. The location of silver nanoparticles inside the onion layer has been monitored by the SERS images collected from chemicals present in the onion and/or reporter molecules attached to the nanoparticles. Our studies show a competitive adsorption of intrinsic bio molecules of the onion layer and reporter molecules. Different spectra from different places of the layer indicate the complex heterogeneous chemical structure of the plant material. The pH-sensitive reporter molecule para mercapto benzoic acid attached to the nanoparticles allows us to infer pH values inside the extracellular matrix of the onion layer.

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Effects of Freezing and Hardening on the Sulfhydryl Groups of Protein Fractions From Cabbage Leaves

Abstract: .4bsttact. Disc

Cited by 17 publications

References 6 publications

Critically assessing the utility of portable lead analyzers for wildlife conservation

Critically assessing the utility of portable lead analyzers for wildlife conservation

Ultrastructural Changes of Plants Exposed to Low Temperatures

Raman spectroscopic probing of plant material using SERS

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