Looking into dental pulp stem cells in the therapy of photoreceptors and retinal degenerative disorders

Alsaeedi, Hiba Amer; Lam, Chenshen; Koh, Avin Ee-Hwan; Teh, Seoh Wei; Mok, Pooi Ling; Higuchi, Akon; Then, K.; Bastion, Mae Lynn Catherine; Alzahrani, Badr; Farhana, Aisha; Muthuvenkatachalam, Bala Sundaram; Samrot, Antony V.; Swamy, K. B.; Marraiki, Najat; Elgorban, Abdallah M.; Subbiah, Suresh Kumar

doi:10.1016/j.jphotobiol.2019.111727

Cited by 8 publications

(7 citation statements)

References 55 publications

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“…Pulpitis and periapical periodontitis are the most frequently diagnosed dental diseases ( 1 , 2 ). Affected by the special histo-physiological characteristics of pulp, it is difficult for the pulp to recover after being damaged ( 3 ).…”

Section: Introductionmentioning

confidence: 99%

“…Affected by the special histo-physiological characteristics of pulp, it is difficult for the pulp to recover after being damaged ( 3 ). At present, root canal treatment is the main strategy for treating pulpitis and periapical periodontitis by applying artificial materials, such as glue tip and paste, to fill the empty root canal for replacing the damaged pulp tissues after cleaning and disinfecting the root canal ( 1 , 4 ). Loss of pulp will reduce blood vessels and nerves, nutrition of the teeth and stress sensing ability.…”

Section: Introductionmentioning

confidence: 99%

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MiR-148a-3p Regulates the Invasion and Odontoblastic Differentiation of Human Dental Pulp Stem Cells via the Wnt1/β-Catenin Pathway

Huang

2021

IJSC

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Background and Objectives MiR-148a-3p has been reported to regulate the differentiation of marrow stromal cell osteoblast. In this study, whether miR-148a-3p regulated the odontoblastic differentiation of human dental pulp stem cells (hDPSCs) or not was explored. Methods and Results The hDPSCs were isolated and identified via flow cytometry. Targets of miR-148a-3p were identified via bioinformatics and dual-luciferase reporter assay. After the cell was cultured in the odontogenic differentiation medium or infected, cell viability, invasion, and odontoblastic differentiation were detected via MTT, transwell, and Alizarin Red S staining, respectively. The miR-148a-3p, Wnt1, β-catenin, DSPP, DMP-1, RUNX2, OCN, and Smad4 expressions were determined by RT-qPCR and Western blot. The hDPSCs odontoblastic differentiation downregulated the miR-148a-3p expression and upregulated Wnt1 expression. Wnt1 was determined as the target for miR-148a-3p. MiR-148a-3p mimic and siWnt1 suppressed the cell viability, invasion, and odontoblastic differentiation of hDPSCs and inhibited the Wnt1, β-catenin, DSPP, DMP-1, RUNX2, OCN, and Smad4 expressions. In contrast, miR-148a-3p inhibitor and overexpressed Wnt1 promoted the cell viability, invasion, and odontoblastic differentiation of hDPSCs, and upregulated the Wnt1, β-catenin, DSPP, DMP-1, RUNX2, OCN, and Smad4 expressions. Also, miR-148a-3p mimic and inhibitor reversed the effects of Wnt1 overexpression and siWnt1. Conclusions MiR-148a-3p modulated the invasion and odontoblastic differentiation of hDPSCs through the Wnt1/β-catenin pathway.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

MiR-148a-3p Regulates the Invasion and Odontoblastic Differentiation of Human Dental Pulp Stem Cells via the Wnt1/β-Catenin Pathway

Huang

2021

IJSC

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“…As a typical neurodegenerative disease, age-related macular degeneration (AMD) and retinitis pigmentosa (RP) bring about visual impairment to millions of people around the world due to degenerative damage of retinal pigment epithelium(RPE)and photoreceptor [ [168] , [169] , [170] ]. However, even after denaturation of RPE and photoreceptor, cells remain intact, and transplant photoreceptors or RPE puts forward an important direction of therapeutic research [ 171 ].…”

Section: Application Of Photo-crosslink Hydrogels In Ophthalmologymentioning

confidence: 99%

Research progress of photo-crosslink hydrogels in ophthalmology: A comprehensive review focus on the applications

Zhang,

Yan,

Zheng

et al. 2024

Materials Today Bio

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“…Surface marker analysis indicated that DPSCs were positive for CD29, CD44, CD73, CD90, CD105, CD117, CD146, CD271, CD166, and STRO-1, but negative for CD11b, CD14, CD19, CD34, CD45, CD79, CD106, and HLA-DR (Table 1) [8][9][10][11][12]. DPSCs were widely used in various clinical applications of regenerative medicine including dentin regeneration, treatment of retinal degeneration, spinal cord injuries, Parkinson's disease, Alzheimer's disease, cerebral ischemia, myocardial infarction, muscular dystrophy, and diabetes and immune diseases [13][14][15][16].…”

Section: Dental Pulp Stem Cellsmentioning

confidence: 99%

Characteristics, Classification, and Application of Stem Cells Derived from Human Teeth

Tong

Zhang

2021

Stem Cells International

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Since mesenchymal stem cells derived from human teeth are characterized as having the properties of excellent proliferation, multilineage differentiation, and immune regulation. Dental stem cells exhibit fibroblast-like microscopic appearance and express mesenchymal markers, embryonic markers, and vascular markers but do not express hematopoietic markers. Dental stem cells are a mixed population with different sensitive markers, characteristics, and therapeutic effects. Single or combined surface markers are not only helpful for understanding the subpopulation of mixed stem cell populations according to cell function but also for improving the stable treatment effect of dental stem cells. Focusing on the discovery and characterization of stem cells isolated from human teeth over the past 20 years, this review outlines the effect of marker sorting on cell proliferation and differentiation ability and the assessment of the clinical application potential. Classified dental stem cells from markers and functional molecules can solve the problem of heterogeneity and ensure the efficacy of cell therapy strategies including dentistry, neurologic diseases, bone repair, and tissue engineering.

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Looking into dental pulp stem cells in the therapy of photoreceptors and retinal degenerative disorders

Cited by 8 publications

References 55 publications

MiR-148a-3p Regulates the Invasion and Odontoblastic Differentiation of Human Dental Pulp Stem Cells via the Wnt1/β-Catenin Pathway

MiR-148a-3p Regulates the Invasion and Odontoblastic Differentiation of Human Dental Pulp Stem Cells via the Wnt1/β-Catenin Pathway

Research progress of photo-crosslink hydrogels in ophthalmology: A comprehensive review focus on the applications

Characteristics, Classification, and Application of Stem Cells Derived from Human Teeth

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