Longitudinal study of CTX-M ESBL-producing E. coli strains on a UK dairy farm

Horton, Robert A.; Duncan, Daisy; Randall, Luke; Chappell, S.; Brunton, Lucy A.; Warner, RG; Coldham, N. G.; Teale, Christopher

doi:10.1016/j.rvsc.2016.09.018

Cited by 24 publications

(25 citation statements)

References 30 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…15.5% in Calf samples, 4.1% in Adult samples). Watson et al (2012) (24) also found that CTX-M prevalence was much higher in calves, but Horton et al (2016) (21) did not report such a difference; however, bla CTX-M status in this latter study was presumptive based on the phenotypic identification of cefotaxime resistance, which would make these results less comparable with those presented here. In other farmed species, Agerso et al (2011) (25) found a prevalence of bla CTX-M E. coli carriage of approximately 7% in Danish slaughter pigs and Randall et al (2010) (26) found a bla CTX-M E. coli prevalence of 3.6% in UK broiler chickens and turkeys.…”

Section: Discussioncontrasting

confidence: 56%

Reduced antibacterial drug resistance and bla_CTX-M β-lactamase gene carriage in cattle-associated Escherichia coli at low temperatures, at sites dominated by older animals and on pastureland: implications for surveillance

Schubert

Morley

Puddy

et al. 2019

Preprint

View full text Add to dashboard Cite

16Little is known about the drivers of critically important antibacterial resistance in 17 species with zoonotic potential present on farms (e.g. CTX-M ꞵ-lactamase-positive 18 Escherichia coli). There is also debate about the influence of farms on the circulation 19 of resistance in local human populations. This was a two-year surveillance study on 20 53 dairy farms. E. coli positive for blaCTX-M were detected in 224/4145 (5.4%) of all 21 samples from faecally-contaminated sites. E. coli positive for blaCTX-M were more 22 prevalent (98/631; 15.5%) in calf samples and less prevalent (12/630; 1.9%) in 23 samples collected from pastureland, including publicly accessible sites. Multilevel, 24 multivariable logistic regression showed antibiotic dry cow therapeutic choice to be 25 associated with risk of blaCTX-M positivity, including use of cefquinome or framycetin; 26 74% of blaCTX-M-positive E. coli were framycetin-resistant. Low temperature was 27 associated with low risk of blaCTX-M positivity. This was additional to the effect of 28 temperature on total E. coli density, a finding with profound implications for 29 surveillance. There was no evidence that study farms had a significant impact on 30 circulating blaCTX-M plasmids in the local human population: across 296 fully 31 sequenced E. coli isolates, two cattle isolates shared blaCTX-M plasmids with eight 32 urinary isolates collected in parallel.33 34 Antimicrobial resistance (AMR) -and particularly antibacterial resistance (ABR) -is a 35 significant global challenge. Many countries are implementing plans to reduce the 36 use of antibacterial drugs (ABs) in food-producing animals. For example, the most 37 recent UK five-year National Action Plan includes a target to reduce AB use (ABU) in 38the treatment of food-producing animals by 25% (1). In Europe, AB sales for food-39 producing animals fell by 20% from 2011 to 2016 (2). In the UK dairy industry, 40 overall ABU dropped from 24 mg/kg in 2015 to 17 mg/kg in 2017 (3, 4). In 2018, 41 additional industry-led policies were enforced in the UK that aim to almost eliminate 42 the use of highest priority critically important antimicrobials (HP-CIAs) such as third-43 and fourth-generation cephalosporins (3GCs and 4GCs) and fluoroquinolones on 44 dairy farms. One reason for reducing ABU in farming is the belief that such 45 measures will reduce the prevalence of ABR bacteria carried by farm animals. 46 However, there is a need for better data on drivers of ABR in farming. More 47 granularity of understanding is required concerning the risks of using individual ABs 48 and other management practices. This is especially important in terms of drivers of 49 HP-CIA resistance. One key focus is on 3GC resistance in Escherichia coli, a 50 species commonly found in animal faeces and considered one of the most significant 51 potential zoonotic threats to humans (5). 523GC-resistance is increasingly common in E. coli causing infections in humans (6) 53 and is also found in farmed and domestic animals around the world (7). The...

show abstract

Section: Discussioncontrasting

confidence: 56%

Reduced antibacterial drug resistance and bla_CTX-M β-lactamase gene carriage in cattle-associated Escherichia coli at low temperatures, at sites dominated by older animals and on pastureland: implications for surveillance

Schubert

Morley

Puddy

et al. 2019

Preprint

View full text Add to dashboard Cite

show abstract

“…As reported in several previous studies, manure generated by dairy cattle may contain potentially harmful antibiotic resistant E. coli [ 36 , 37 , 38 ]. The emergence and spread of ESBL-producing E. coli associated with dairy cattle manure are of particular concern [ 39 , 40 , 41 , 42 , 43 ]. In the present study the antibiotic resistance profiles of TIO and FOX resistant E. coli isolates from dairy cattle manure samples were determined.…”

Section: Discussionmentioning

confidence: 99%

Genomic Analysis of Third Generation Cephalosporin Resistant Escherichia coli from Dairy Cow Manure

Rehman

Lepp

et al. 2017

Veterinary Sciences

View full text Add to dashboard Cite

The production of extended-spectrum β-lactamases (ESBLs) conferring resistance to new derivatives of β-lactams is a major public health threat if present in pathogenic Gram-negative bacteria. The objective of this study was to characterize ceftiofur (TIO)- or cefotaxime (FOX)-resistant Escherichia coli isolated from dairy cow manure. Twenty-four manure samples were collected from four farms and incubated under anaerobic conditions for 20 weeks at 4 °C or at 25 °C. A total of 37 TIO- or FOX-resistant E. coli were isolated from two of the four farms to determine their susceptibility to 14 antibiotics. Among the 37 resistant E. coli , 10 different serotypes were identified, with O8:H1 being the predominant serotype ( n = 17). Five isolates belonged to each of serotypes O9:NM and O153:H42, respectively. All 37 cephalosporin resistant isolates were multi-resistant with the most prevalent resistance spectrum being amoxicillin-clavulanic acid-ampicillin-cefoxitin-ceftiofur-ceftriaxone-chloramphenicol-streptomycin-sulfisoxazole-tetracycline-trimethoprim-sulfamethoxazole. The genomes of 18 selected isolates were then sequenced and compared to 14 selected human pathogenic E. coli reference genomes obtained from public repositories using different bioinformatics approaches. As expected, all 18 sequenced isolates carried at least one β-lactamase bla gene: TEM-1 , TEM-81 , CTX-M115 , CTX-M15 , OXA-1, or CMY-2 . Several other antibiotic resistance genes (ARGs) and virulence determinants were detected in the sequenced isolates and all of them harbored antimicrobial resistance plasmids belonging to classic Inc groups. Our results confirm the presence of diverse ESBL producing E. coli isolates in dairy cow manure stored for a short period of time. Such manure might constitute a reservoir of resistance and virulence genes for other bacteria that share the same environment.

show abstract

“…; Horton et al . ). Different strains were defined by using a cut‐off of 80% similarity as per published guidance (Tenover et al .…”

Section: Methodsmentioning

confidence: 97%

Detection of extended‐spectrum β‐lactam, AmpC and carbapenem resistance in Enterobacteriaceae in beef cattle in Great Britain in 2015

et al. 2019

Self Cite

View full text Add to dashboard Cite

Aims: This study investigated the occurrence and genetic diversity of Enterobacteriaceae with extended-spectrum b-lactamase (ESBL)-, AmpC-and carbapenemase-mediated resistance in British beef cattle, and related risk factors. Methods and Results: Faecal samples (n = 776) were obtained from farms in England and Wales (n = 20) and Scotland (n = 20) in 2015. Isolates from selective agars were identified by MALDI ToF mass spectrometry. Selected isolates were characterized by multiplex PCR (bla CTX-M, bla OXA, bla SHV and bla TEM genes), whole-genome sequencing (WGS), minimum inhibitory concentrations and pulsed-field gel electrophoresis. None of the faecal samples yielded carbapenem-resistant Escherichia coli. Ten (25%) of the farms tested positive for ESBL-producing CTX-M Enterobacteriaceae, 15 (37Á5%) of the farms were positive for AmpC phenotype E. coli and none were positive for carbapenem-resistant E. coli. WGS showed a total of 30 different resistance genes associated with E. coli, Citrobacter and Serratia from ESBL agars, and colocation of resistance genes with bla CTX-M1 . Buying bulls and bringing in fattening cattle from another farm were identified as significant risk factors for positive samples harbouring CTX-M Enterobacteriaceae or AmpC phenotype E. coli respectively. Conclusions: Beef cattle on a proportion of farms in GB carry ESBLproducing Enterobacteriaceae. Factors, such as operating as a closed herd, may have an important role in reducing introduction and transmission of resistant Enterobacteriaceae. The results indicate management factors may play an important role in impacting ESBL prevalence. In particular, further study would be valuable to understand the impact of maintaining a closed herd on reducing the introduction of resistant Enterobacteriaceae. Significance and Impact of the Study: This is the first study showing the presence of ESBL-producing Enterobacteriaceae in British beef cattle.

show abstract

Longitudinal study of CTX-M ESBL-producing E. coli strains on a UK dairy farm

Cited by 24 publications

References 30 publications

Reduced antibacterial drug resistance and bla_CTX-M β-lactamase gene carriage in cattle-associated Escherichia coli at low temperatures, at sites dominated by older animals and on pastureland: implications for surveillance

Reduced antibacterial drug resistance and bla_CTX-M β-lactamase gene carriage in cattle-associated Escherichia coli at low temperatures, at sites dominated by older animals and on pastureland: implications for surveillance

Genomic Analysis of Third Generation Cephalosporin Resistant Escherichia coli from Dairy Cow Manure

Detection of extended‐spectrum β‐lactam, AmpC and carbapenem resistance in Enterobacteriaceae in beef cattle in Great Britain in 2015

Contact Info

Product

Resources

About

Longitudinal study of CTX-M ESBL-producing E. coli strains on a UK dairy farm

Cited by 24 publications

References 30 publications

Reduced antibacterial drug resistance and blaCTX-M β-lactamase gene carriage in cattle-associated Escherichia coli at low temperatures, at sites dominated by older animals and on pastureland: implications for surveillance

Reduced antibacterial drug resistance and blaCTX-M β-lactamase gene carriage in cattle-associated Escherichia coli at low temperatures, at sites dominated by older animals and on pastureland: implications for surveillance

Genomic Analysis of Third Generation Cephalosporin Resistant Escherichia coli from Dairy Cow Manure

Detection of extended‐spectrum β‐lactam, AmpC and carbapenem resistance in Enterobacteriaceae in beef cattle in Great Britain in 2015

Contact Info

Product

Resources

About

Reduced antibacterial drug resistance and bla_CTX-M β-lactamase gene carriage in cattle-associated Escherichia coli at low temperatures, at sites dominated by older animals and on pastureland: implications for surveillance

Reduced antibacterial drug resistance and bla_CTX-M β-lactamase gene carriage in cattle-associated Escherichia coli at low temperatures, at sites dominated by older animals and on pastureland: implications for surveillance