Detection of extended‐spectrum β‐lactam, AmpC and carbapenem resistance in Enterobacteriaceae in beef cattle in Great Britain in 2015

Abstract

Aims: This study investigated the occurrence and genetic diversity of Enterobacteriaceae with extended-spectrum b-lactamase (ESBL)-, AmpC-and carbapenemase-mediated resistance in British beef cattle, and related risk factors. Methods and Results: Faecal samples (n = 776) were obtained from farms in England and Wales (n = 20) and Scotland (n = 20) in 2015. Isolates from selective agars were identified by MALDI ToF mass spectrometry. Selected isolates were characterized by multiplex PCR (bla CTX-M, bla OXA, bla SHV and bla TEM genes), whole-genome sequencing (WGS), minimum inhibitory concentrations and pulsed-field gel electrophoresis. None of the faecal samples yielded carbapenem-resistant Escherichia coli. Ten (25%) of the farms tested positive for ESBL-producing CTX-M Enterobacteriaceae, 15 (37Á5%) of the farms were positive for AmpC phenotype E. coli and none were positive for carbapenem-resistant E. coli. WGS showed a total of 30 different resistance genes associated with E. coli, Citrobacter and Serratia from ESBL agars, and colocation of resistance genes with bla CTX-M1 . Buying bulls and bringing in fattening cattle from another farm were identified as significant risk factors for positive samples harbouring CTX-M Enterobacteriaceae or AmpC phenotype E. coli respectively. Conclusions: Beef cattle on a proportion of farms in GB carry ESBLproducing Enterobacteriaceae. Factors, such as operating as a closed herd, may have an important role in reducing introduction and transmission of resistant Enterobacteriaceae. The results indicate management factors may play an important role in impacting ESBL prevalence. In particular, further study would be valuable to understand the impact of maintaining a closed herd on reducing the introduction of resistant Enterobacteriaceae. Significance and Impact of the Study: This is the first study showing the presence of ESBL-producing Enterobacteriaceae in British beef cattle.

Extended-spectrum beta-lactamase (ESBL)-producing Enterobacteriaceae in cattle production – a threat around the world

“…Faecal (Velasova et al, 2019) Infection (Hunter et al, 2010) ND (Stokes, 2014) Czech Republic Faecal (Dolejska et al, 2011b) Sick (Dolejska et al, 2013) R eunion ND (Gay et al, 2018) Slovakia Faecal (Kme t and Buj n akov a, 2018) Sweden Faecal (Duse et al, 2015) Switzerland Faecal (Endimiani et al, 2012;Geser et al, 2012a;Zurfluh et al, 2015) Turkey Faecal (AslantaŞ et al, 2017;Pehlivanoglu et al, 2016) North America Canada ND (Cormier et al, 2019) USA Faecal (Mir et al, 2016;Mollenkopf et al, 2012;Wittum et al, 2010) Asia China Mastitis (Ali et al, 2016(Ali et al, , 2017 South Korea Mastitis (Tark et al, 2017) Indonesia Faecal (Sudarwanto et al, 2016) Japan Mastitis (Ohnishi et al, 2013b) Klebsiella pneumoniaeEurope Italy Mastitis (Locatelli et al, 2010) Klebsiella ozaenae Italy Faecal (Stefani et al, 2014) Salmonella enterica Germany ND (Rodríguez et al, 2009) CTX-M-1/61 E. coli Europe Germany Faecal (Dahms et al, 2015) CTX-M-2 E. coli South America Brazil Faecal (Palmeira et al, 2018) Europe Germany Mastitis (Eisenberger et al, 2017;Freitag et al, 2017;Michael et al, 2017) Sick (Michael et al, 2017) Netherlands Faecal (Ceccarelli et al, 2019;Heuvelink et al, 2019) North America Canada Faecal (Cormier et al, 2016) Asia Japan Faecal (Shiraki et al, 2004) Diarrheic (Ohnishi et al, 2013a) Infection (Asai et al, 2011) K. pneumoniae Japan Mastitis (Ohnishi et...…”

Genomic network analysis of environmental and livestock F-type plasmid populations

“…Environmental (non-clinical and non-human) populations of Enterobacterales may act as a genetic reservoir for antimicrobial resistance (AMR). This includes livestock [1][2][3][4][5] and water-borne [6] resistance. Frequent horizontal gene transfer (HGT) in Enterobacterales populations results in a large and open pangenome, enabling the wide-spread transmission of the genes conferring AMR [7][8][9].…”

Antimicrobial Resistance Patterns of Escherichia coli Isolated from Sheep and Beef Farms in England and Wales: A Comparison of Disk Diffusion Interpretation Methods

“…There are few studies investigating antimicrobial susceptibility of organisms isolated from healthy sheep and beef cattle in the UK. These studies suggest that antimicrobial Antibiotics 2021, 10, 453 2 of 17 resistance on sheep and beef farms is relatively uncommon [8][9][10], although extended spectrum beta-lactamase (ESBL)-positive beef farms may be increasing [11,12]. Examining the presence of ESBL E. coli has been the focus of more recent studies on beef farms [11,12].…”