Longitudinal profile of the genotoxic potential of the River Danube on erythrocytes of wild common bleak (Alburnus alburnus) assessed using the comet and micronucleus assay

Deutschmann, Björn; Kolarević, Stoimir; Brack, Werner; Kaišarević, Sonja; Kostić, Jovana; Kračun-Kolarević, Margareta; Liška, Igor; Paunović, Momír; Seiler, Thomas‐Benjamin; Shao, Ying; Sipos, Sándor; Slobodnı́k, Jaroslav; Teodorović, Ivana; Vuković-Gačić, Branka; Hollert, Henner

doi:10.1016/j.scitotenv.2016.07.175

Cited by 39 publications

(32 citation statements)

References 44 publications

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“…We therefore identify a seasonality of the damage measured thanks to the MN rate. Similar seasonality of MN rate that parallels the variation of contaminants levels in natural environment has already been reported in fish (Costa et al 2011;D'Costa et al 2017;Deutschmann et al 2016;Hussain et al 2018;Lee and Steinert 2003;Monserrat et al 2007).…”

Section: Genome Integritysupporting

confidence: 81%

Health indicators and contaminant levels of a critically endangered species in the Gironde estuary, the European sturgeon

Acolas

Davail

Gonzalez

et al. 2019

Environ Sci Pollut Res

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The European sturgeon, Acipenser sturio, is a highly endangered species that almost disappeared in the last decades. Thanks to yearly restocking of the population, this species is still found in the Gironde estuary (France), where juveniles grow during several years before leaving to the ocean. The aim of this study was to evaluate the pressure exerted on these fish by known organic and inorganic contaminants during their stay at the Gironde estuary, and to get information on the fish's health in this context. Monthly captures over the year 2014, provided 87 fish from the cohorts 2012 and 2013 mainly, and from cohorts 2008, 2009 and 2011, all fish born in hatchery. We report the very first analyses of contaminant levels and of biological markers measured in the blood of these fish. Low inorganic contamination was found, composed of seven metals mainly: Zn (< 5 µg.mL-1), Fe (< 1.5 µg.mL-1), Cu (< 0.8 µg.mL-1), Se (< 0.8 µg.mL-1), As (< 0.25 µg.mL-1), Co (< 0.14 µg.mL-1) and Mn (< 0.03 µg.mL-1). Concerning persistent organic contaminants, the sum of 7 PCBs varied from 1 to 10 ng.g-1 plasma, that of 8 OCPs from 0.1 to 1 ng.g-1 , and that of 8 PBDEs from 10 to 100 pg.g-1. Higher levels of contaminants were measured during spring as compared to summer. The sex steroid hormone plasma levels (estradiol, testosterone and 11ketotestosterone) were quite low, which 2 was predictable for juveniles. The transcription of reproduction-involved genes (EstR, AR, LHR, sox9) in blood cells was demonstrated for the first time. Some of them were correlated to organic contaminant levels PCBs and OCPs. Other gene transcriptions (sodCu and bax) were correlated to PCBs and OCPs. However, the DNA damage level measured here as comet tail DNA and micronuclei ratio in red blood cells, were in the very low range of the values commonly obtained in fish from pristine areas. The data presented here can serve as a reference base for future monitoring of this population of sturgeons.

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Section: Genome Integritysupporting

confidence: 81%

Health indicators and contaminant levels of a critically endangered species in the Gironde estuary, the European sturgeon

Acolas

Davail

Gonzalez

et al. 2019

Environ Sci Pollut Res

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“…These include mainly chromosomal aberrations, sister chromatid exchange, comet assay, and micronucleus assay. Micronucleus (MN) and comet assay are the most popular and promising tests for monitoring the environmental genotoxicity and are being served as an index of cytogenetic damage (Deutschmann et al 2016;Ruiz de Arcaute et al 2016). Vasquez (2010) recently emphasized that at least two assays should be used for in vivo and in vitro studies.…”

Section: Introductionmentioning

confidence: 99%

Widely used non-ionic surfactant 4-nonylphenol: showing genotoxic effects in various tissues of Channa punctatus

Sharma

Chadha

2017

Environ Sci Pollut Res

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The present study investigated the intertissue differences in genotoxicity induction in the fish, Channa punctatus, in response to 4-nonylphenol. The lethal concentration of 50 (LC) was estimated using a semi-static system. Fish were subjected to three sublethal concentrations of 4-nonylphenol (NP) for 24, 48, 72, and 96 h. Liver, gill, and kidney tissues were analyzed, and the genotoxicity was estimated using the micronucleus test and the comet assay. The frequency of micronucleated cells (MNCs), binucleated cells (BNCs), aberrant cells, and tail moment (TM) increased significantly. The time for maximum induction of genotoxicity for all the parameters considered in the micronucleus assay was 72 h of exposure, whereas the TM was highest at 24 h of exposure followed by a decline. The gill and liver showed higher genotoxicity than kidney tissue demonstrating organ-specific susceptibilities to NP in fish C. punctatus.

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“…Results from a battery of in situ biomarkers in sentinel fish (Alburnus alburnus and Neogobius sp.) [17], including mainly markers for exposure (enzyme activities for biotransformation, oxidative stress and neuronal activation) and effects at cellular levels (genotoxicity), were analyzed and aggregated using an index for the average biomarker response. Finally, taxonomy-and trait-based analyses of fish and macroinvertebrate community data were performed to indicate ecological impacts [56].…”

Section: Ecology-directed Analysismentioning

confidence: 99%

Future water quality monitoring: improving the balance between exposure and toxicity assessments of real-world pollutant mixtures

et al. 2019

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Environmental water quality monitoring aims to provide the data required for safeguarding the environment against adverse biological effects from multiple chemical contamination arising from anthropogenic diffuse emissions and point sources. Here, we integrate the experience of the international EU-funded project SOLUTIONS to shift the focus of water monitoring from a few legacy chemicals to complex chemical mixtures, and to identify relevant drivers of toxic effects. Monitoring serves a range of purposes, from control of chemical and ecological status compliance to safeguarding specific water uses, such as drinking water abstraction. Various water sampling techniques, chemical target, suspect and non-target analyses as well as an array of in vitro, in vivo and in situ bioanalytical methods were advanced to improve monitoring of water contamination. Major improvements for broader applicability include tailored sampling techniques, screening and identification techniques for a broader and more diverse set of chemicals, higher detection sensitivity, standardized protocols for chemical, toxicological, and ecological assessments combined with systematic evidence evaluation techniques. No single method or combination of methods is able to meet all divergent monitoring purposes. Current monitoring approaches tend to emphasize either targeted exposure or effect detection. Here, we argue that, irrespective of the specific purpose, assessment of monitoring results would benefit substantially from obtaining and linking information on the occurrence of both chemicals and potentially adverse biological effects. In this paper, we specify the information required to: (1) identify relevant contaminants, (2) assess the impact of contamination in aquatic ecosystems, or (3) quantify cause-effect relationships between contaminants and adverse effects. Specific strategies to link chemical and bioanalytical information are outlined for each of these distinct goals. These strategies have been developed and explored using case studies in the Danube and Rhine river basins as well as for rivers of the Iberian Peninsula. Current water quality assessment suffers from biases resulting from differences in approaches and associated uncertainty analyses. While exposure approaches tend to ignore data gaps (i.e., missing contaminants), effect-based approaches penalize data gaps with increased uncertainty factors. This integrated work suggests systematic ways to deal with mixture exposures and combined effects in a more balanced way, and thus provides guidance for future tailored environmental monitoring. which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.

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Longitudinal profile of the genotoxic potential of the River Danube on erythrocytes of wild common bleak (Alburnus alburnus) assessed using the comet and micronucleus assay

Cited by 39 publications

References 44 publications

Health indicators and contaminant levels of a critically endangered species in the Gironde estuary, the European sturgeon

Health indicators and contaminant levels of a critically endangered species in the Gironde estuary, the European sturgeon

Widely used non-ionic surfactant 4-nonylphenol: showing genotoxic effects in various tissues of Channa punctatus

Future water quality monitoring: improving the balance between exposure and toxicity assessments of real-world pollutant mixtures

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