Longitudinal columnar organization within the dorsal motor nucleus represents separate branches of the abdominal vagus

Fox, Edward A.; Powley, Terry L.

doi:10.1016/0006-8993(85)91066-2

Cited by 189 publications

(103 citation statements)

References 21 publications

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“…The overwhelming ipsilateral pattern of nodose infection is consistent with the general view that the distal process, soma, and proximal process of pseudounipolar cells of the nodose ganglia remain uncrossed, and thus ipsilateral to the tissue they innervate (and their origin in the nodose ganglia) in rodents (e.g., 19). The nodose labeling, although suggestive of parasympathetic (PSNS) afferents, is instead more likely secondary to infection of WAT spinal afferents projecting to the dorsal vagal complex of the brainstem, an area where we saw HSV-1-ir at 96 h, which, in turn, have descending projections to the nodose ganglia (e.g., 23).…”

Section: Discussionsupporting

confidence: 83%

Anterograde transneuronal viral tract tracing reveals central sensory circuits from white adipose tissue

Song¹,

Schwartz²,

Bartness³

2009

American Journal of Physiology-Regulatory, Integrative and Comp

115

131

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Song CK, Schwartz GJ, Bartness TJ. Anterograde transneuronal viral tract tracing reveals central sensory circuits from white adipose tissue. Am J Physiol Regul Integr Comp Physiol 296: R501-R511, 2009. First published December 24, 2008 doi:10.1152/ajpregu.90786.2008The origins of the sympathetic nervous system (SNS) innervation of white adipose tissue (WAT) have been defined using the transneuronal viral retrograde tract tracer, pseudorabies virus. Activation of this SNS innervation is acknowledged as the principal initiator of WAT lipolysis. The central control of WAT lipolysis may require neural feedback to a brain-SNS-WAT circuit via WAT afferents. Indeed, conventional tract tracing studies have demonstrated that peripheral pseudounipolar dorsal root ganglion (DRG) sensory cells innervate WAT. The central nervous system projections of WAT afferents remain uncharted, however, and form the focus of the present study. We used the H129 strain of the herpes simplex virus-1 (HSV-1), an anterograde transneuronal viral tract tracer, to define the afferent circuits projecting from WAT to the central nervous system. Siberian hamster inguinal (IWAT) or epididymal WAT was injected with H129 and the neuraxis processed for HSV-1 immunoreactivity. We found substantial overlap in the pattern of WAT sensory afferent projections with multiple SNS outflow sites along the neuraxis, suggesting the possibility of WAT sensory-SNS circuits that could regulate WAT SNS drive and thereby lipolysis. Previously, we demonstrated that systemic 2-deoxy-D-glucose (2DG) elicited increases in the SNS drive to IWAT. Here, we show that systemic 2DG administration also significantly increases multiunit spike activity arising from decentralized IWAT afferents. Collectively, these data provide structural and functional support for the existence of a sensory WAT pathway to the brain, important in the negative feedback control of lipid mobilization.2-deoxy-D-glucose; lipolysis; sympathetic nervous system; electrophysiology FEEDBACK FROM LIPID STORES to the central nervous system (CNS) has been suggested to contribute to the relative stability of total body fat in some individuals, but not in others (for a review, see Ref. 1). Historically, the idea of a lipid feedback signal became prominent with Kennedy's "lipostatic theory" (24), and this notion apparently was affirmed with the discovery of leptin, the largely adipocyte-derived cytokine thought by some to reflect total body fat stores (for a review, see Ref. 21). As with any feedback system, one controlling lipid stores would require not only afferent signals from white adipose tissue (WAT), but also effectors to increase or decrease the lipid stores appropriately. As with any feedback system, one controlling lipid stores would require not only afferent signals from WAT, but also effectors to increase or decrease the lipid stores appropriately. Whether such a system exists or needs to exist for total body fat to be stable is debatable (46). Efferent neural control of WAT is well recognized. We and...

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Section: Discussionsupporting

confidence: 83%

Anterograde transneuronal viral tract tracing reveals central sensory circuits from white adipose tissue

Song¹,

Schwartz²,

Bartness³

2009

American Journal of Physiology-Regulatory, Integrative and Comp

115

131

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“…By doing so, Jarvinen and Powley might have skewed their analysis toward gastric-projecting DMV neurons. In fact, gastric-projecting neurons project to the viscera via three of the five subdiaphragmatic vagal branches, which originate in the medial two-third of the DMV [10,18,21]. Our sampling technique, instead, focuses on DMV neurons that are identified as projecting to specific visceral targets, and it is more prone to uncover, if present, morphological differences between anatomically distinct neuronal subgroups.…”

Section: Discussionmentioning

confidence: 99%

Morphological differences between planes of section do not influence the electrophysiological properties of identified rat dorsal motor nucleus of the vagus neurons

2004

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Recent cytoarchitectonic studies have shown that the dorsal motor nucleus of the vagus (DMV) comprises neurons with different morphological features. Our own studies, conducted in horizontal brainstem slices, have shown that DMV neurons projecting to stomach areas can be distinguished from neurons projecting to the intestine on the basis of their electrophysiological as well as morphological properties. The majority of the in vitro experimental investigations, however, have been conducted on coronal brainstem slices. The aim of the present study was to assess whether the electrophysiological properties of DMV neurons are due to intrinsic membrane properties of the neurons or are dependent upon the plane of section, i.e., coronal vs. horizontal, in which the brainstem is cut. The fluorescent retrograde tracer DiI was applied to either the stomach or intestine of rats. Whole cell recordings were subsequently made from labeled DMV neurons in thin brainstem slices sectioned in either the horizontal or coronal plane. In the horizontal plane, both the somata and the dendritic tree of gastric-projecting neurons were smaller than intestinalprojecting neurons. In the coronal plane, however, apart from a smaller soma diameter in gastricprojecting neurons, morphological differences were not found between the groups. The electrophysiological differences observed between the groups were, however, consistent in both planes of section, that is, intestinal-projecting neurons had larger and longer afterhyperpolarization (AHP) as well as slower frequency-responses to depolarizing stimuli than gastric-projecting neurons. Our data suggest that intrinsic rather than morphological features govern the electrophysiological characteristics of DMV neurons.

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“…Rats (n ϭ 14) were injected with fluorogold (20 g/1 ml saline, i.p., per rat; Fluorochrome, Denver, CO) to label vagal preganglionic neurons innervating the subdiaphragmatic viscera, thus allowing delineation of the boundaries of the DMV (Fox and Powley, 1985;Zheng et al, 1999;Guo et al, 2001). Three days later, the rats were anesthetized, the brainstem was extracted as described above, and 200 m thick slices were allowed to recover for 90 min in oxygenated Krebs' at 32°C.…”

Section: Methodsmentioning

confidence: 99%

μ-Opioid Receptor Trafficking on Inhibitory Synapses in the Rat Brainstem

Browning

Kalyuzhny

Travagli³

2004

J. Neurosci.

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Longitudinal columnar organization within the dorsal motor nucleus represents separate branches of the abdominal vagus

Cited by 189 publications

References 21 publications

Anterograde transneuronal viral tract tracing reveals central sensory circuits from white adipose tissue

Anterograde transneuronal viral tract tracing reveals central sensory circuits from white adipose tissue

Morphological differences between planes of section do not influence the electrophysiological properties of identified rat dorsal motor nucleus of the vagus neurons

μ-Opioid Receptor Trafficking on Inhibitory Synapses in the Rat Brainstem

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