Morphological differences between planes of section do not influence the electrophysiological properties of identified rat dorsal motor nucleus of the vagus neurons

Valenzuela, Isabel; Browning, Kirsteen N.; Travagli, R. Alberto

doi:10.1016/j.brainres.2003.10.076

Cited by 21 publications

(9 citation statements)

References 28 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In the mouse, these include a small number of GABAergic neurons. Overall, the properties of neurons retrogradely labeled subsequent to inoculation of the stomach with PRV-152 or PRV-614 were similar to those reported previously for gastric-projecting DMV cells (Browning et al, 1999; Valenzuela et al, 2004), validating this labeling technique in these cells. Whereas retrogradely transported labels such as FluoroGold are as likely to be taken up by injured axons or axons of passage as by terminals, PRV preferentially infects terminal fields over axons of passage (Card et al, 1993; Ch’ng et al, 2007), resulting in a larger pool of cells selectively labeled gastric-related neurons.…”

Section: Discussionsupporting

confidence: 85%

“…Putative interneurons with smaller somata have also been reported in the nucleus (McLean and Hopkins, 1981; 1982; Blessing et al, 1984; Blessing, 1990; Izzo et al, 1992; Fox and Powley, 1992; Huang et al, 1993; Streefland et al, 1998; Jarvinen and Powley, 1999), but axon trajectories of these cells have not been well-characterized. Several studies have indicated that DMV motor neurons have a small but detectable degree of heterogeneity with regard to functional, morphological, electrophysiological attributes (Huang et al, 1993; Fogel et al, 1996; Browning et al, 1999; Valenzuela, et al, 2004). The morphological details of putative interneurons have never been reported (Jarvinen and Powley, 1999).…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Morphological and electrophysiological features of motor neurons and putative interneurons in the dorsal vagal complex of rats and mice

et al. 2009

View full text Add to dashboard Cite

The dorsal motor nucleus of the vagus (DMV) contains preganglionic motor neurons that control viscera along the subdiaphragmatic digestive tract, but may also contain neurons that do not project to the viscera. Neurons that expressed EGFP 60-72 h subsequent to PRV-152 inoculation of vagal terminals in the stomach wall were targeted for whole-cell patch-clamp recording and biocytin filling in transverse brainstem slices from rats and their quantitative morphological and electrophysiological characteristics were compared with uninfected cells. Over 90% of PRV-152 labeled neurons were also labeled subsequent to intraperitoneal injection of FluoroGold, indicating most were preganglionic motor neurons. In reconstructed neurons with an identifiable axon trajectory, two cellular subtypes were distinguished. The axon projected ventrolaterally from the DMV in 44 of 49 cells and these were likely to be vagal motor neurons. Axons of other neurons ramified within the nucleus tractus solitarius (NTS) or DMV. These cells were smaller and otherwise morphologically distinct from putative motor neurons. Transgenic mice with GFP-expressing inhibitory neurons (i.e., GIN mice) were used to identify a GABAergic subset vagal neurons. These neurons had locallyramifying axons and formed a morphologically distinct subset of DMV cells, which were similar in size and axon trajectory to GABAergic neurons in the NTS. Most neurons in the DMV therefore possess morphological features of motor neurons, but locally projecting cells and inhibitory neurons with distinct morphological features are also found within the DMV. These cells likely contribute to regulation of vagal function.

show abstract

Section: Discussionsupporting

confidence: 85%

Section: Introductionmentioning

confidence: 99%

Morphological and electrophysiological features of motor neurons and putative interneurons in the dorsal vagal complex of rats and mice

et al. 2009

View full text Add to dashboard Cite

show abstract

“…Furthermore, Huang et al, have suggested that within the human brainstem, DMV neurons display distinctive morphological properties and are organized into functionally specific groups that innervate particular visceral organs (239). In support of this, studies in rodents have demonstrated that DMV neurons are morphologically heterogeneous (74, 169, 252, 317) and our studies have shown that the biophysical and morphological properties of DMV neurons are correlated with their target organ within the GI tract (74). In brief, it appears that DMV neurons projecting to the gastric fundus are smaller and have fewer dendritic branches as compared to other GI-projecting DMV neurons, with neurons projecting to the large intestine having the largest soma size (74,317).…”

Section: Properties and Organization Of Vagal Efferent Motoneuronssupporting

confidence: 80%

“…In support of this, studies in rodents have demonstrated that DMV neurons are morphologically heterogeneous (74, 169, 252, 317) and our studies have shown that the biophysical and morphological properties of DMV neurons are correlated with their target organ within the GI tract (74). In brief, it appears that DMV neurons projecting to the gastric fundus are smaller and have fewer dendritic branches as compared to other GI-projecting DMV neurons, with neurons projecting to the large intestine having the largest soma size (74,317). In addition, functional studies have also demonstrated that DMV neurons sensitive to gastric distention are morphologically different from those that respond to intestinal distention (164).…”

Section: Properties and Organization Of Vagal Efferent Motoneuronssupporting

confidence: 80%

Central Nervous System Control of Gastrointestinal Motility and Secretion and Modulation of Gastrointestinal Functions

Browning

Travagli

2014

Comprehensive Physiology

Self Cite

425

388

View full text Add to dashboard Cite

Although the gastrointestinal (GI) tract possesses intrinsic neural plexuses that allow a significant degree of autonomy over GI functions, the central nervous system (CNS) provides extrinsic neural inputs that regulate, modulate, and control these functions. While the intestines are capable of functioning in the absence of extrinsic inputs, the stomach and esophagus are much more dependent upon extrinsic neural inputs, particularly from parasympathetic and sympathetic pathways. The sympathetic nervous system exerts a predominantly inhibitory effect upon GI muscle and provides a tonic inhibitory influence over mucosal secretion while, at the same time, regulates GI blood flow via neurally mediated vasoconstriction. The parasympathetic nervous system, in contrast, exerts both excitatory and inhibitory control over gastric and intestinal tone and motility. Although GI functions are controlled by the autonomic nervous system and occur, by and large, independently of conscious perception, it is clear that the higher CNS centers influence homeostatic control as well as cognitive and behavioral functions. This review will describe the basic neural circuitry of extrinsic inputs to the GI tract as well as the major CNS nuclei that innervate and modulate the activity of these pathways. The role of CNS-centered reflexes in the regulation of GI functions will be discussed as will modulation of these reflexes under both physiological and pathophysiological conditions. Finally, future directions within the field will be discussed in terms of important questions that remain to be resolved and advances in technology that may help provide these answers.

show abstract

“…Soma Surface, Number of Branch. As natural morphology is closely related to neuronal characteristics and functions [1], [2], [3], [4], neuroscientists make great efforts to study on neurons from the perspective of neuronal morphology, and have achieved fruitful achievements [5], [6], [7], [8].…”

Section: Introductionmentioning

confidence: 99%

A universal neuronal classification and naming scheme based on the neuronal morphology

Xie

2011

Proceedings of 2011 International Conference on Computer Science and Network Technology

View full text Add to dashboard Cite

Neuronal morphology, which is closely related to neuronal characteristics and functions, is complex and diversified, and it has gradually attracted more and more neuroscientists to study on it. As the neuronal classification is a basic point in neuronal study but no existing universal methods take neuronal morphology into consideration, this paper dedicates to fill this blank. Firstly, this paper used Principal Component Analysis (PCA) method to select five key features from twenty neuronal morphologic features. With these key features, this paper leveraged hierarchical clustering to cluster sixty neurons randomly selected from the NeuroMorpho.Org website. As a result, these neurons were divided into four categories. Finally, we devised a new naming scheme by the range of key features' values. Experiments indicated that this classification can effectively distinguish neurons by morphology. At last, this paper discussed and analyzed the anomaly that occurs after a large number of classification experiments. I. I NTRODUCTIONHuman brain, which contains hundreds of billions of neurons, has the most complex structure and functions in a living body. As neuron is the base unit of brain, it's significant to study neurons thoroughly.Neuronal morphology is mainly referred to neurons' spacial conformation, generally including dendrite recelvmg information, soma dealing with information and axon sending information. There are also many morphologic features to describe neuronal morphology quantitatively, e.g. Soma Surface, Number of Branch. As natural morphology is closely related to neuronal characteristics and functions [1], [2], [3], [4], neuroscientists make great efforts to study on neurons from the perspective of neuronal morphology, and have achieved fruitful achievements [5], [6], [7], [8].Neuronal classification is the basic point in neuronal research. As all the neuronal morphology can be measured by some common standards [9], it's practical to classify neurons based on neuronal morphology. We note that some neuroscientists have already studied on the neurons by classifying the neurons morphologically.978-1-4577-1587-711l1$26.00

show abstract

Morphological differences between planes of section do not influence the electrophysiological properties of identified rat dorsal motor nucleus of the vagus neurons

Cited by 21 publications

References 28 publications

Morphological and electrophysiological features of motor neurons and putative interneurons in the dorsal vagal complex of rats and mice

Morphological and electrophysiological features of motor neurons and putative interneurons in the dorsal vagal complex of rats and mice

Central Nervous System Control of Gastrointestinal Motility and Secretion and Modulation of Gastrointestinal Functions

A universal neuronal classification and naming scheme based on the neuronal morphology

Contact Info

Product

Resources

About