Longevity of Salmonella typhimurium in Tilapia aurea and water from pools fertilized with swine waste

Abstract

Salmonella typhimurium declined rapidly when inoculated into Tilapia aurea culture pools fertilized with fresh swine waste. Within the water column, a 95% decline of viable cells occurred during the first 6 h. Isolation of viable salmonellae was possible at 16 days post-inoculation, but not at 32 days. Similarly, salmonellae could be detected in the viscera and epithelium of T. aurea at 16 days, although not at 32 days. Salmonellae were not isolated from the fish flesh, nor was there evidence of septicemic infection.

Human impact on the microbiological water quality of the rivers

“…Salmonella spp., enteropathogenic E. coli and Vibrio spp., represent important pathogens. The genus Salmonella comprises more than 2400 serotypes, most of which are considered as an endemic public-health concern worldwide (Baker et al, 1983, Baggesen et al, 2000Soto et al, 2006). Salmonella enterica serovar Typhimurium, diagnosed by random amplified polymorphic DNA typing, antimicrobial resistance, and plasmid and integron profiles, represents a common cause of enteric disease in many countries (Soto et al, 2006), and has been involved in water-borne disease of humans and animals (Dondero et al, 1977;Davies, 2001;Kariuki et al, 1999;Mmolawa et al, 2002;Martinez-Urtaza et al, 2004).…”

Immune response of Tilapia aurea exposed to Salmonella typhimurium

“…Suspect Salmonella colonies were identified as to genus by reactions in triple sugar iron agar, urea agar, and lysine decarboxylase broth and by their ability to produce indole and agglutinate Polyvalent "O" antiserum (11). With this procedure, we were able to detect as few as two viable cells (3).…”

Livestock Waste Management

“…Wide diurnal fluctuations in dissolved oxygen and pH make survival impossible for many of the pathogenic bac teria such as Salmonella spp. (Baker, 1981). Thus fish grown in manure-fertilized systems are free of many of the pathogenic bacteria associated with the manure.…”