Long‐term survival of sclerotia of <i>Rhizoctonia tuliparum</i>

Coley‐Smith, J. R.; Humphreys-Jones, D. R.; Gladders, P.

doi:10.1111/j.1365-3059.1979.tb02626.x

Cited by 8 publications

(6 citation statements)

References 2 publications

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“…They found that the proportion of sclerotia germinating following retrieval from field soil was 45% in the top 1 cm of soil, 19% between 1 and 5 cm from the soil surface and 6% between 15 and 20 cm. For another Rhizoctonia sp., R. tuliparum, 25 and 36% of sclerotia retrieved from 0 and 7.5 cm depth were viable after 7 years, compared to 9 and 29% at 15-to 22.5-cm depth (Coley-Smith et al 1979).…”

Section: Discussionmentioning

confidence: 88%

“…Sclerotia produced by Rhizoctonia tuliparum were found to survive burial for over 10 years, although the percentage of sclerotia germination following retrieval was <10% (Coley‐Smith et al. ). Sclerotial survival in other anastomosis groups of R. solani has also been investigated.…”

Section: Discussionmentioning

confidence: 99%

“…For another Rhizoctonia sp., R. tuliparum , 25 and 36% of sclerotia retrieved from 0 and 7.5 cm depth were viable after 7 years, compared to 9 and 29% at 15‐ to 22.5‐cm depth (Coley‐Smith et al. ).…”

Section: Discussionmentioning

confidence: 99%

“…Previous studies have demonstrated that sclerotia produced by other Rhizoctonia species such as Rhizoctonia cerealis survived burial in field soil for 6 months (Pitt 1964). Sclerotia produced by Rhizoctonia tuliparum were found to survive burial for over 10 years, although the percentage of sclerotia germination following retrieval was <10% (Coley-Smith et al 1979). Sclerotial survival in other anastomosis groups of R. solani has also been investigated.…”

Section: Discussionmentioning

confidence: 99%

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Survival of Sclerotia of Rhizoctonia solani AG3PT and Effect of Soil‐Borne Inoculum Density on Disease Development on Potato

Ritchie

Bain

McQuilken³

2012

Journal of Phytopathology

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Sclerotia produced by a single isolate of Rhizoctonia solani AG3PT were buried in small plot experiments to investigate the effects of sclerotial production method, soil type and burial depth on sclerotial viability in field soil. The factor with the greatest effect on sclerotial viability, defined as the percentage of sclerotia germinating on agar following retrieval, in all experiments was the duration of burial. After 18 months, on average across all experiments, 20% of retrieved sclerotia were viable. A comparison between sclerotia produced in vitro on malt yeast extract agar and in vivo using micropropagated tubers in field soil found no significant differences between the two production methods on sclerotial viability. Burial in field soil at 20‐cm depth was found to significantly reduce sclerotial viability to 50% compared to 60% at 5 cm. In two pot experiments, amending the growing medium and soil with increasing inoculum densities of R. solani was found to increase stem number, stem canker and black scurf severity regardless of whether this soil‐borne inoculum was derived from mycelium or sclerotia. Black scurf incidence and severity were assessed 30–32 days posthaulm destruction and found to be similar for a range of sclerotial soil‐borne inoculum densities (1.0 × 10−1 g/kg d.w. soil to 6 × 10−3 g/kg d.w. soil). The significance of these findings in relation to pathogen survival, detection in soil and disease development is discussed.

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Section: Discussionmentioning

confidence: 88%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

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Survival of Sclerotia of Rhizoctonia solani AG3PT and Effect of Soil‐Borne Inoculum Density on Disease Development on Potato

Ritchie

Bain

McQuilken³

2012

Journal of Phytopathology

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“…They turn from white to dark brown or black as they ripen and can be found on bulbs and in surrounding soil. According to Coley-Smith et al [10], about 10% of the sclerotia can remain viable in soil for at least ten years. Sclerotia usually remain attached to soil and plant debris when the bulbs are dug, allowing them to be easily spread from field to field by cultivating equipment.…”

Section: Introductionmentioning

confidence: 99%

Detection and Molecular Phylogenetic-Morphometric Characterization of Rhizoctonia tuliparum, Causal Agent of Gray Bulb Rot of Tulips and Bulbous Iris

Coats

DeBauw

Lakshman

et al. 2022

JoF

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Gray bulb rot of tulips and bulbous iris is caused by the soil-borne fungal pathogen, Rhizoctonia tuliparum (Rtul). Sclerotia present in infected bulbs, as well as overwintering sclerotia in soil and field debris, are the primary sources of infection. A method for accurate and sensitive detection of Rtul from soil and infected bulbs, and estimation of inoculum threshold levels, is needed for the management of disease caused by this pathogen. We designed a unique set of primers targeting the ITS2 region of the Rtul genome and developed a highly sensitive quantitative PCR (qPCR)-based method for Rtul identification using these primers, where the threshold of detection was approximately 1 fg Rtul DNA. The assay was more sensitive with sclerotia collected from the field (natural) than with those grown in the lab, and more sensitive with natural-light than natural-dark sclerotia. Also, the detection method was more sensitive when sclerotia were extracted from soil than from bulb tissue. The qPCR method was highly specific, as no PCR amplification was detected when genomic DNA from 62 non-Rtul Rhizoctonia isolates from a wide range of anastomosis groups were tested. To understand the evolutionary relationships and genomic diversity of Rtul, we performed phylogenetics of the ITS1-5.8S-ITS2 region and ITS2-molecular morphometric characterization (MMC) of Rtul isolates. The three Rtul isolates whose ITS sequences were available in GenBank formed a distinct phylogenetic clade with Ceratobasidium anceps as the nearest relative. Furthermore, MMC analysis revealed genetic divergence among these three Rtul isolates.

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Flowerbulbs Diseases Incited by Rhizoctonia Species

Dijst¹,

Schneider²

1996

Rhizoctonia Species: Taxonomy, Molecular Biology, Ecology, Pathology and Disease Control

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Long‐term survival of sclerotia of Rhizoctonia tuliparum

Cited by 8 publications

References 2 publications

Survival of Sclerotia of Rhizoctonia solani AG3PT and Effect of Soil‐Borne Inoculum Density on Disease Development on Potato

Survival of Sclerotia of Rhizoctonia solani AG3PT and Effect of Soil‐Borne Inoculum Density on Disease Development on Potato

Detection and Molecular Phylogenetic-Morphometric Characterization of Rhizoctonia tuliparum, Causal Agent of Gray Bulb Rot of Tulips and Bulbous Iris

Flowerbulbs Diseases Incited by Rhizoctonia Species

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