Long-term study of ovine pulmonary adenocarcinogenesis in sheep with marginal vs. sufficient nutritional selenium supply: Results from computed tomography, pathology, immunohistochemistry, JSRV-PCR and lung biochemistry

Humann-Ziehank, Esther; Renko, Kostja; Bruegmann, Michael Ludwig; Devi, V. Rama; Hewicker‐Trautwein, M.; Andreae, Arnim; Ganter, Martin

doi:10.1016/j.jtemb.2013.03.002

Cited by 13 publications

(15 citation statements)

References 45 publications

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“…An earlier study [25] suggested that about 25% of total serum Se is associated with GPx. Our results indicated that this proportion might be even lower (9.61% in Se + and 15.7 % in Se -) being in accordance to 16.7 % reported previously using the SAX-ICP-DRC-MS method in human serum [12]. The occurrence of SeVI, Se-Met, Se-OSA, Se-TrxR and SeIV in ovine serum was likely, but was not analysed in this species before and was quantified here for the first time.…”

Section: Se and Se Species In Serumsupporting

confidence: 75%

“…Due to one main focus of the comprehensive study, all sheep underwent general anaesthesia to perform computed tomography (CT) of the lungs [16] at day 750 of the study. As it is impossible to collect CSF by puncture of the ependymal canal in sheep without general anaesthesia due to resistance of the animals and subsequent risk of cerebrospinal damage, the anaesthetic stage (for the CT) was used to collect the CSF samples.…”

Section: Blood and Csf Collectionsmentioning

confidence: 99%

“…Detailed data of the experimental animals were published earlier [4,16]. In brief, during the whole experiment the sheep showed no abnormalities of behaviour and general health.…”

Section: General Condition Of the Experimental Animalsmentioning

confidence: 99%

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Selenium speciation in paired serum and cerebrospinal fluid samples of sheep

Humann-Ziehank

Ganter

Michalke

2016

Journal of Trace Elements in Medicine and Biology

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This study was performed to characterise selenium (Se) and Se species in cerebrospinal fluid (CSF) of sheep and its relation to the respective Se concentrations in serum. Paired samples from 10 adult sheep were used for the study. Five sheep were fed a diet with a marginal Se concentration of <0.05mg Se/kg diet dry weight (dw, Se(-)), and five animals were fed the same diet supplemented with sodium selenite revealing a concentration of 0.2mg Se/kg diet dw (Se(+)). The feeding strategy was conducted for two years; The results on metabolic effects were published previously. At the end of the feeding period, paired samples of serum and CSF were collected and analysed using ion exchange chromatography inductively coupled plasma-dynamic reaction cell-mass spectrometry (IEC-ICP-DRC-MS) technique for total Se concentration and concentrations of Se species. Albumin concentrations were analysed additionally. The feeding strategy caused significant differences (p<0.01) in serum Se concentrations with 33.1±5.11μg Se/l in the Se(-) group and 96.5±18.3μg Se/l in the Se(+) group, respectively. The corresponding total Se concentrations in CSF were 4.38±1.02μg Se/l and 6.13±1.64μg Se/l in the Se(-) and the Se(+) group, respectively, missing statistical significance (p=0.077). IEC-ICP-DRC-MS technique was able to differentiate the Se species selenoprotein P-bound Se (SePP), selenomethionine, glutathione peroxidase-bound Se (Se-GPx), selenocystine, thioredoxin reductase-bound Se, ovine serum albumin-bound Se (Se-OSA), SeIV and SeVI in ovine serum and CSF. Quantitatively, SePP is the main selenoprotein in ovine serum followed by Se-GPx. The CSF/blood ratio of albumin (QAlbumin) reflected a physiological function of the blood-CSF barrier in all sheep. QSe-species were higher than QAlbumin both feeding groups, supporting the hypothesis of local production of Se species in the brain. Significant positive regression lines for CSF vs. serum were found for albumin and Se-OSA only, suggesting a role of albumin to convey Se across the blood-CSF barrier. The ovine model, together with the IEC-ICP-DRC-MS technique to characterise the Se species, might be a worthwhile model for further studies as repeated sample collection as well as modification of the nutritional status is feasible and effective.

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Section: Se and Se Species In Serumsupporting

confidence: 75%

Section: Blood and Csf Collectionsmentioning

confidence: 99%

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Selenium speciation in paired serum and cerebrospinal fluid samples of sheep

Humann-Ziehank

Ganter

Michalke

2016

Journal of Trace Elements in Medicine and Biology

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“…A subsequent study analysed the results of sufficient (0.2 mg Se/kg dw) vs. marginal (0.05 mg Se/kg dw) nutritional Se supplementation on OPA progression over a two-year period. A coherency of cancer progression with nutritional Se supplementation was not evident in this study design, however, JSRV induced OPA in combination with score-based quantification of tumor progression using CT examination was found to be a valuable large animal model for lung cancer progression in general [113].…”

Section: Se Metabolism In Animals-usage As An Animal Modelmentioning

confidence: 68%

Selenium, copper and iron in veterinary medicine—From clinical implications to scientific models

Humann-Ziehank

2016

Journal of Trace Elements in Medicine and Biology

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“…However, other authors claim that no evidence of efficacy of selenium supplementation in prevention of lung cancer was found (7,9). Generally, this issue seems to be unclear and researchers point to the necessity of avoiding supranutritional dose of selenium (11).…”

Section: Introductionmentioning

confidence: 99%

Comparison of the influence of selenium supplementation in organic and inorganic forms on oxidant/antioxidant balance in rat lungs

Musik

Kiełczykowska

Kocot

2013

Bulletin of the Veterinary Institute in Pulawy

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The aim of the experiment was to compare the effect of two newly synthesised organic selenocompounds with that exerted by sodium selenite on oxidant processes in rat lungs. Total antioxidant status (TAS), activity of antioxidant enzymes -superoxide dismutase (SOD) and glutathione peroxidase (GPx), concentrations of non-enzymatic antioxidants -ascorbic acid (AA) and reduced glutathione (GSH), concentration of lipid peroxidation marker -malonyldialdehyde (MDA), as well as tissue concentrations of silicon and magnesium were determined in rats receiving different selenocompounds (inorganic selenite and organic selenosemicarbazide in a chain form -compound A and selenazoline in a ring form -compound B). TAS values were elevated in comparison with control without Se-supplementation. GPx was insignificantly increased when compared to control, mainly in the group receiving compound B. Inorganic selenite significantly increased SOD and decreased levels of AA. MDA was slightly altered in Se-supplemented animals. Silicon levels were not affected, whereas magnesium concentrations were considerably reduced in all groups receiving selenium. Compound B increased TAS to the highest degree. It did not have any impact on components of the antioxidant barrier and slightly decreased MDA. Therefore, it could be suggested that further research, including in vitro studies on cancer cell lines, may reveal new possibilities of medical applications of selenocompounds.

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Long-term study of ovine pulmonary adenocarcinogenesis in sheep with marginal vs. sufficient nutritional selenium supply: Results from computed tomography, pathology, immunohistochemistry, JSRV-PCR and lung biochemistry

Cited by 13 publications

References 45 publications

Selenium speciation in paired serum and cerebrospinal fluid samples of sheep

Selenium speciation in paired serum and cerebrospinal fluid samples of sheep

Selenium, copper and iron in veterinary medicine—From clinical implications to scientific models

Comparison of the influence of selenium supplementation in organic and inorganic forms on oxidant/antioxidant balance in rat lungs

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