Long-term stability of glucose: 96-h study using Terumo Glycaemia tubes

Winter, Theresa; Greiser, Anne; Nauck, Matthias; Petersmann, Astrid

doi:10.1515/cclm-2015-0548

Cited by 12 publications

(4 citation statements)

References 11 publications

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“…In conclusion, the authors of the present study agree on the fact that, following a good laboratory practice, in order to do a correct diagnosis of DM, and above all of GDM, trying to avoid wrong classifications of patients, the use of an immediate inhibitor of the glycolysis has to be regarded as necessary ( 27 , 28 ). The FC mixture in Terumo Venosafe Glycemia tubes ( 10 , 17 , 22 ), from the data presented seem to be suitable for this purpose, while NaF alone is not.…”

Section: Discussionmentioning

confidence: 89%

“…In the light of the present and recently published results ( 17 , 22 - 24 ), it might emerge the impellent need of undertaking new standardized and pre-analytically well-performed studies using FC-mixture tubes with the aim of redefining DM cut-offs. The use of acidified tubes could bring to an increase of the average glucose value of the population, as it was highlighted in recent studies ( 25 , 26 ).…”

Section: Discussionmentioning

confidence: 96%

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Effectiveness of citrate buffer-fluoride mixture in Terumo tubes as an inhibitor of in vitro glycolysis

et al. 2016

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IntroductionGlycolysis affects glucose determination in vitro. The placement of sample tubes in ice-water slurry with plasma separation within 30 minutes is recommended, or alternatively the use of a glycolysis inhibitor. The aim of our two-steps study was to evaluate which Terumo tube is best for glucose determination in routine clinical setting.Materials and methodsIn the first study, blood from 100 volunteers was collected into lithium heparin (LH), NaF/Na heparin (FH) and NaF/citrate buffer/Na2EDTA (FC-Mixture) tubes. LH sample was treated as recommended and considered as reference, while FH and FC-Mixture samples were aliquoted, maintained at room temperature (RT) for 1, 2 and 4 hours; centrifuged and plasma analysed in triplicate. In the second study, samples from 375 volunteers were collected in LH, FH and FC-Mixture tubes and held at RT before centrifugation from 10 to 340 minutes, depending on each laboratory practice. Samples were analysed in one analytical run.ResultsIn the first study, FH glucose concentrations were 5.15 ± 0.66 mmol/L, 5.05 ± 0.65 mmol/L and 5.00 ± 0.65 mmol/L (P < 0.001) in tubes stored at RT for 1, 2 and 4 hours, respectively. Mean biases in all time points exceeded the analytical goal for desirable bias based on biological variation criteria. FC-Mixture glucose concentrations were 5.48 ± 0.65 mmol/L, 5.46 ± 0.6 mmol/L and 5.46 ± 0.64 mmol/L in tubes stored at RT for 1, 2 and 4 hours, respectively. Mean biases for FC-Mixture glucose in all time points reached optimal analytical goals. In the second study, the biases for LH and FH glucose compared to reference FC-Mixture glucose exceeded the preset analytical goals, regardless of the blood collection to centrifugation time interval.ConclusionsFC-mixture tubes glucose concentrations were preserved up to 4h storage at RT. We confirmed that NaF alone does not allow immediate glycolysis inhibition in real life pre-centrifugation storage conditions (up to 340 minutes). FC-Mixture should be used exclusively for glucose determination in laboratories unable to implement the recommended blood samples’ treatment.

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Section: Discussionmentioning

confidence: 89%

Section: Discussionmentioning

confidence: 96%

Effectiveness of citrate buffer-fluoride mixture in Terumo tubes as an inhibitor of in vitro glycolysis

et al. 2016

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“…Winter et al . demonstrated a long-term stability of glucose up to 96 h at RT with a continuous shaking, when Terumo tubes were used ( 10 ). In this study, a lower stability was found for the same.…”

Section: Discussionmentioning

confidence: 99%

The new Greiner FC-Mix tubes equal the old Terumo ones and are useful as glucose stabilizer after prolonged storage of samples

Bonetti

Carta

2017

Biochemia Medica

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IntroductionThe aim of our study is to compare new Greiner tubes containing granulated citrate buffer with the Terumo ones and to verify if they are suitable for glucose stabilization after prolonged storage.Materials and methodsIn Study 1, blood was collected in two Terumo and two Greiner tubes from 40 healthy volunteers. Samples were stored at room temperature (RT) for 1 and 2 hours, respectively. Comparison was made by Deming regression. In Study 2, glucose was measured in a reference tube (N = 50), according to the ADA-NACB guidelines and in aliquots of Greiner samples maintained un-centrifuged at RT for 1, 2, 4 (N = 50) and 24, 48, 72 hours (N = 35).ResultsThere were insignificant mixed biases between the Terumo and Greiner tubes. Compared to reference (5.3 mmol/L), glucose concentration in the new tubes was 5.4 (P < 0.05), 5.4 (P < 0.05), 5.3 (P = 0.265), 5.2 (P = 0.156), 5.3 (P < 0.05) and 5.2 (P < 0.05) mmol/L after 1, 2, 4, 24, 48 and 72 hours at RT, respectively. There was no biological difference between any of the time points up to 48 h (bias < ± 1.95%).ConclusionsThe study shows that the new tubes perform equally well as the Terumo ones and ensure glucose stabilization up to 48 h as well as permit to create a link between the previous studies demonstrating the clinical utility of granulated citrate buffer and the future ones.

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“…7 Additives like sodium fluoride citrate can be used to effectively inhibit glycolysis, 6 but long-term stability over more than 72 hours is not well established. 8,9 In addition, care has to be taken during blood sampling and handling of samples. Alternatively, whole blood samples may be centrifuged to separate cells from plasma.…”

Section: Introductionmentioning

confidence: 99%

Stability of Glucose Concentrations in Frozen Plasma

Pleus

Freckmann

Baumstark

et al. 2020

J Diabetes Sci Technol

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Background: Conflicting information is available regarding the stability of glucose concentrations in frozen plasma samples. Clinical trials could benefit from such long-term storage because it would allow usage of a central laboratory with higher-quality laboratory analyzers in contrast to mobile analyzers in a decentralized setting. Methods: In this study, venous blood samples were collected in lithium-heparin gel tubes. Plasma was separated immediately after blood was drawn, and from each of the 21 plasma samples, 6 aliquots were prepared for measurement at 6 time points: immediately and after 2, 4, 6, 8, and 12 weeks. Between sampling and measurement, aliquots were stored at less than −20°C. Transport on dry ice was simulated by placing aliquots in a −80°C freezer for 5 days between weeks 8 and 12. Measurements were performed on a hexokinase-based laboratory analyzer. Average relative differences and corresponding 99% confidence intervals (CIs) were calculated between the stored aliquots’ and the immediately measured aliquots’ glucose concentrations. Glucose concentrations were deemed stable as long as average relative differences were ≤±2.5%. Results: Over the whole 12-weeks duration, the largest average relative difference was −1.82% (99% CI: –2.25% to −1.39%). Shorter storage durations tended to lead to less bias. Conclusion: In this study, the stability of glucose concentrations in frozen plasma samples obtained with lithium-heparin gel tubes could be shown for up to 12 weeks. Future studies should be performed to assess whether this is independent of the glucose analyzer and the type of sampling tube used.

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Long-term stability of glucose: 96-h study using Terumo Glycaemia tubes

Cited by 12 publications

References 11 publications

Effectiveness of citrate buffer-fluoride mixture in Terumo tubes as an inhibitor of in vitro glycolysis

Effectiveness of citrate buffer-fluoride mixture in Terumo tubes as an inhibitor of in vitro glycolysis

The new Greiner FC-Mix tubes equal the old Terumo ones and are useful as glucose stabilizer after prolonged storage of samples

Stability of Glucose Concentrations in Frozen Plasma

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