Long term shear effects on a hybridoma cell line by dynamic perfusion devices

Scale-down studies for assessing the impact of different stress parameters on growth and product quality during animal cell culture

“…Perfusion culture has been frequently studied and often involves the use of pumps to recirculate the cells. Pump damage was found to reduce the viability of a variety of animal cell types, including insect (Merten, 2000;Gorenflo et al, 2004), hybridoma (LaPorte et al, 1996) and CHO cells (Kim et al, 2008). Yet, in perfusion culture, the rate of pumping is far lower than here, circulation rates being about 1 to 10 bioreactor volumes/day (Merten, 2000;Kim et al, 2008) compared to ~ 100 to ~ 200 per day here.…”