Long‐Term Prevalence of Antimicrobial‐Resistant<i>Salmonella enterica</i>Subspecies<i>enterica</i>Serovar Infantis in the Broiler Chicken Industry in Japan

Asai, Tetsuo; Ishihara, Katsuya; Harada, Kazuki; Kojima, Akira; Tsutsumi, Yutaka; Sato, Shizuo; Takahashi, Toshio

doi:10.1111/j.1348-0421.2007.tb03881.x

Cited by 24 publications

(19 citation statements)

References 16 publications

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“…However, molecular characterisation, if performed, demonstrated that this phenotype was due to resistance genes different from those described in this paper or located on the chromosome [12,13,21,22].…”

Section: Discussionmentioning

confidence: 58%

Molecular characterisation of multidrug-resistant Salmonella enterica serotype Infantis from humans, animals and the environment in Italy

Dionisi

Lucarelli

Benedetti

et al. 2011

International Journal of Antimicrobial Agents

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Section: Discussionmentioning

confidence: 58%

Molecular characterisation of multidrug-resistant Salmonella enterica serotype Infantis from humans, animals and the environment in Italy

Dionisi

Lucarelli

Benedetti

et al. 2011

International Journal of Antimicrobial Agents

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“…Highly similar antibiotic resistance characteristic of the strains were indicating the clonal dissemination of these clusters. The emergence of multidrug-resistant clones in S. Infantis in broilers and humans was reported in several countries (Marimon et al 2004;Shahada et al 2006;Asai et al 2007). All S. Infantis strains presented class I integrons with the variable regions of 1.0 kb and 1.2 kb.…”

Section: Resultsmentioning

confidence: 96%

Phenotypic and genetic characterization of multidrug-resistant Salmonella Infantis strains isolated from broiler chicken meats in Turkey

Abbasoglu

Akçelik

2011

Biologia

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Twenty Salmonella Infantis strains resistant against kanamycin, tetracycline, neomycin, spectinomycin, sulphonamide, nalidixic acid and trimethoprim were selected for this study out of 103 Salmonella strains isolated from broiler samples collected from several markets in the Bolu and Ankara regions of Turkey. The resistance genes aadA1, aphA1, sul1, tet(A), dfrA5/dfrA14 and gyrA were determined for these multidrug-resistant S. Infantis strains. S. Infantis strains contained a mega plasmid with the molecular size of 206 kb. The strains were divided into three groups according to the pulsed field gel electrophoresis patterns of XbaI digested chromosomal DNA. A Ser83→Tyr83 point mutation was found in the gyrA gene of all quinolone-resistant isolates. Filter mating experiments showed that 206 kb plasmid transferred nalidixic acid resistance associated with class I integrons.

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“…Moreover, related studies (Hamada et al, 2003b;Kudaka et al, 2006;Asai et al, 2007) described Salmonella Infantis as the most frequent isolate in broilers, and their genotypes showed a close resemblance. Despite the chromosome profile similarity observed with XbaI, we revealed changes consistent with a single genetic event when BlnI was used.…”

Section: Discussionmentioning

confidence: 91%

“…In the present study the most frequent serotype recovered in chicken was not the most common serovar isolated from cases of human salmonellosis. The most common isolate from chicken since 1997 has been Salmonella Infantis, whereas in humans the most common isolate was Salmonella Enteritidis (Asai et al, 2007). According to the recent Japanese Infectious Agents Surveillance report, Salmonella Infantis has been the second most common human isolate (http:// idsc.nih.go.jp/iasr/index.html Accessed Nov. 2007).…”

Section: Discussionmentioning

confidence: 98%

“…Nontyphoid human infections are most often acquired from contaminated foods of animal origin. Salmonella Infantis and Salmonella Typhimurium have been major causes of salmonellosis in humans in Japan until 1989 when Salmonella Enteritidis suddenly emerged and continued prevailing to date (Hamada et al, 2003a;Asai et al, 2007). 968 holdings (n = 9), positive-flocks from persistently infected holdings (n = 21), and holdings (n = 19) that showed fluctuations with alternating negative and positive flocks for variable time periods.…”

Section: Introductionmentioning

confidence: 97%

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Temporal Distribution and Genetic Fingerprinting of Salmonella in Broiler Flocks from Southern Japan

et al. 2008

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During the 1998 to 2003 period, cecal contents of 4,024 broiler chickens from 252 flocks raised in 63 holdings were examined for Salmonella. The aims were to establish the actual status of the infection, its temporal distribution, prevalent serotype, and common genotype among broiler flocks brought at the slaughterhouse. Collected samples were preenriched in Hajna tetrathionate broth, and after 24 h of incubation, 10 microL of the broth was streaked on selective Rambach agar plate. Suspected scarlet color colonies of Salmonella were cloned on nutrient agar, confirmed through biochemical tests and sero-typed using O and H antigens. Pulsed field gel electrophoresis technique generated DNA fragments banding patterns and established their clonal relatedness. Salmonella was isolated from 563 (14%) samples in 179 (71%) flocks. The flock situation varied from Salmonella-negative holdings (n = 9), positive-flocks from persistently infected holdings (n = 21), and holdings (n = 19) that showed fluctuations with alternating negative and positive flocks for variable time periods. Fourteen holdings (negative, n = 5 and positive, n = 9) were sampled once throughout the study period. Seasonality component was not observed, and salmonellae were found colonizing broiler ceca in warm and cold months. Predominant serovar was Salmonella Infantis (93.3%; n = 525). Macrorestriction fingerprints of Salmonella Infantis using XbaI presumed the isolates to be derived from a common parent. Enhanced discrimination by BlnI digestion produced 3 banding patterns that were closely related genetically and hence epidemiologically related. Such epidemiological information may enable producers to formulate effective control action plan tailored for individual holdings with special emphasis on biosecurity, hygiene, and pest control.

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Long‐Term Prevalence of Antimicrobial‐ResistantSalmonella entericaSubspeciesentericaSerovar Infantis in the Broiler Chicken Industry in Japan

Cited by 24 publications

References 16 publications

Molecular characterisation of multidrug-resistant Salmonella enterica serotype Infantis from humans, animals and the environment in Italy

Molecular characterisation of multidrug-resistant Salmonella enterica serotype Infantis from humans, animals and the environment in Italy

Phenotypic and genetic characterization of multidrug-resistant Salmonella Infantis strains isolated from broiler chicken meats in Turkey

Temporal Distribution and Genetic Fingerprinting of Salmonella in Broiler Flocks from Southern Japan

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